Decocting is the earliest and most popular method of preparing herbal
medicines in the practice of traditional Chinese medicine. Different
kinds of herbs are mixed together and boiled with water to get decoction,
which are usually took by patients themselves. One popular approach
nowadays is to prepare water extract of individual herbs in the form of
granule. The granule of an individual herb is called a dispensing granule.
The application of granules has the advantages of better quality control
and easier administration. Now the formula granule had been widely
applied in clinic in China, Japan and Korea [1]. However, the chemical
constituents of the formula granule and traditional decoction were similar,
the contents of some constituents and other diversity comparisons may
cause the difference of effectiveness.
Sheng-mai-yin which is composed of Radix Ginseng, Fructus
Schisandrae and Radix Ophiopogoni is a famous traditional Chinese
medicine prescription. Many clinical reports have demonstrated the
cardio-protective effects of Sheng-mai-yin against heart attack and
chronic lesions [2]. However, few chemical comparison has been reported
between dispensing granule and traditional decoction of Sheng-mai-yin.
Therefore, HPLC-UV and HPLC-ESI-MS were applied to analyze the
changes of compositions in each crude drug before and after compatibility.
And then, some pharmacodynamics indicators were compared between
Sheng-mai-yin dispensing granule and traditional decoction by animal
experiments.
The main results were as followed:
The fingerprint of Ginseng Radix et Rhizoma in Sheng-mai-yin was
established by HPLC-UV and HPLC-ESI-MS. Sheng-mai-yin single and
co-decoction were also analyzed under this condition (Figure 1). The
HPLC-ESI-MS results was listed in table 1, which showed that most of
the high and medium-polar ginsenosides in Sheng-mai-yin co-decoction
vanished away, such as Notoginsenoside Rg1
, Rb1
, R1
, Rb2
, Ra1
, Ra2
, Ra3
, Rc,
Rd and Re. Meanwhile, the contents of low-polars ginsenosides increased,
for example, Rh4
, Rs3
, Rg6
, Rg5
, Rg3
and its isomers.
Figure 1: The comparison of chromatograms between Ginseng Radix et Rhizoma, Sheng-mai-yin single and Sheng-mai-yin co-decoction with gradient
elution program. (a) The separated decotion of Ginseng Radix et Rhizoma, (b) The single decotion of Sheng-mai-yin, (c) The co-decotion of Shengmai-yin
HPLC-UV, as well as HPLC-ESI-MS was used to develop the fingerprint
of Schisandrae Chinensis Fructus in Sheng-mai-yin. The medium-polar
compositions and low-polar parts in Schisandrae Chinensis Fructus almost
had no change between single and co-decoction, except the generation of
some new chromatographic peaks in high-polar components in Shengmai-yin
co-decoction (Figure 2). In other words, the compositions in
Schisandrae Chinensis Fructus are impacted weakly by Ginseng Radix
et Rhizoma and Ophiopogonis Radix in co-decoction. Secondly, the
fingerprint of Ophiopogonis Radix in Sheng-mai-yin was established by
HPLC-UV, too. The other two drugs also can hardly affect Ophiopogonis
Radix in Sheng-mai-yinco-decoction (Figure 3).
Figure 2: The comparision of chromatograms between Schisandrae Chinese Fructus, Sheng-mai-yin single decoction and Shengmaiyin co-decoction.
(a) The separated decotion of Schisandrae Chinese Fructus, (b) The single decotion of Sheng-mai-yin, (c) The co-decotion of Sheng-mai-yin
Figure 3: The compared of chromatograms between Ophiopogonis Radix in Sheng-mai-yin single decoction and Sheng-mai-yin co-decoction. (a) The
separated decotion of Ophiopogonis Radix, (b) The single decotion of Sheng-mai-yin, (c) The co-decotion of Sheng-mai-yin
Pharmacodynamics effects of Sheng-mai-yin single and co-decoction
were studied, through the myocardial hypoxia and arrhythmia model
of the mice. Single decoction has stronger anti-myocardial anoxia
effect than co-decoction. Also, the high dose of Sheng-mai-yin codecoction
and the low dose of single decoction can significantly
extend the start time of arrhythmias in mice induced by BaCl2
. They
can significantly inhibit the incidence rate of ventricular fibrillation
induced by chloroform in mice.
Table 1: The Comparison of the types of Ginsenoside between Gingseng Single Decoction and Sheng-Mai-Yin Co-Decoction by HPLC-ESI-MS
HPLC-UV and HPLC-ESI-MS based chemical profiling method was
proposed and validated to rapidly evaluate the chemical constituents of
Sheng-mai-yin single and co-decoction. Compared with single decoction,
the most visible change is that the high and medium-polar ginsenosides
in Sheng-mai-yin co-decoction vanished away, such as Notoginsenoside
Rg1
, Rb1
, R1
, Rb2
, Ra1
, Ra2
, Ra3
, Rc, Rd and Re. Meanwhile, the chemical
compositions of Fructus Schisandrae and Radix Ophiopogoni almost had
no change.
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Article Information
Article Type: Letter to Editor
Citation: Liu JL, Wang XD, Lv CN, Lu JC (2016) The
Comparative Study of Quality Between Sheng-MaiYin
Single and Co-Decoction. J Pharm Anal Insights
1(2): doi http://dx.doi.org/10.16966/2471-8122.106
Copyright: © 2016 Liu JL, et al. This is an
open-access article distributed under the terms
of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.
Publication history:
Received date: 01 Dec 2015
Accepted date: 25
Mar 2016
Published date: 29 Mar 2016