Figure 1: The comparison of chromatograms between Ginseng Radix et Rhizoma, Sheng-mai-yin single and Sheng-mai-yin co-decoction with gradient elution program. (a) The separated decotion of Ginseng Radix et Rhizoma, (b) The single decotion of Sheng-mai-yin, (c) The co-decotion of Shengmai-yin
Jian-Li Liu Xiao-Dan Wang Chong-Ning Lv Jin-Cai Lu*1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
*Corresponding author: Jin-Cai Lu, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang Liaoning 110016, P.R. China, Tel: +024 23986500; E-mail: email@example.com
Decocting is the earliest and most popular method of preparing herbal medicines in the practice of traditional Chinese medicine. Different kinds of herbs are mixed together and boiled with water to get decoction, which are usually took by patients themselves. One popular approach nowadays is to prepare water extract of individual herbs in the form of granule. The granule of an individual herb is called a dispensing granule. The application of granules has the advantages of better quality control and easier administration. Now the formula granule had been widely applied in clinic in China, Japan and Korea . However, the chemical constituents of the formula granule and traditional decoction were similar, the contents of some constituents and other diversity comparisons may cause the difference of effectiveness.
Sheng-mai-yin which is composed of Radix Ginseng, Fructus Schisandrae and Radix Ophiopogoni is a famous traditional Chinese medicine prescription. Many clinical reports have demonstrated the cardio-protective effects of Sheng-mai-yin against heart attack and chronic lesions . However, few chemical comparison has been reported between dispensing granule and traditional decoction of Sheng-mai-yin. Therefore, HPLC-UV and HPLC-ESI-MS were applied to analyze the changes of compositions in each crude drug before and after compatibility. And then, some pharmacodynamics indicators were compared between Sheng-mai-yin dispensing granule and traditional decoction by animal experiments.
The main results were as followed:
The fingerprint of Ginseng Radix et Rhizoma in Sheng-mai-yin was established by HPLC-UV and HPLC-ESI-MS. Sheng-mai-yin single and co-decoction were also analyzed under this condition (Figure 1). The HPLC-ESI-MS results was listed in table 1, which showed that most of the high and medium-polar ginsenosides in Sheng-mai-yin co-decoction vanished away, such as Notoginsenoside Rg1 , Rb1 , R1 , Rb2 , Ra1 , Ra2 , Ra3 , Rc, Rd and Re. Meanwhile, the contents of low-polars ginsenosides increased, for example, Rh4 , Rs3 , Rg6 , Rg5 , Rg3 and its isomers.
HPLC-UV, as well as HPLC-ESI-MS was used to develop the fingerprint of Schisandrae Chinensis Fructus in Sheng-mai-yin. The medium-polar compositions and low-polar parts in Schisandrae Chinensis Fructus almost had no change between single and co-decoction, except the generation of some new chromatographic peaks in high-polar components in Shengmai-yin co-decoction (Figure 2). In other words, the compositions in Schisandrae Chinensis Fructus are impacted weakly by Ginseng Radix et Rhizoma and Ophiopogonis Radix in co-decoction. Secondly, the fingerprint of Ophiopogonis Radix in Sheng-mai-yin was established by HPLC-UV, too. The other two drugs also can hardly affect Ophiopogonis Radix in Sheng-mai-yinco-decoction (Figure 3).
Figure 2: The comparision of chromatograms between Schisandrae Chinese Fructus, Sheng-mai-yin single decoction and Shengmaiyin co-decoction. (a) The separated decotion of Schisandrae Chinese Fructus, (b) The single decotion of Sheng-mai-yin, (c) The co-decotion of Sheng-mai-yin
Figure 3: The compared of chromatograms between Ophiopogonis Radix in Sheng-mai-yin single decoction and Sheng-mai-yin co-decoction. (a) The separated decotion of Ophiopogonis Radix, (b) The single decotion of Sheng-mai-yin, (c) The co-decotion of Sheng-mai-yin
Pharmacodynamics effects of Sheng-mai-yin single and co-decoction were studied, through the myocardial hypoxia and arrhythmia model of the mice. Single decoction has stronger anti-myocardial anoxia effect than co-decoction. Also, the high dose of Sheng-mai-yin codecoction and the low dose of single decoction can significantly extend the start time of arrhythmias in mice induced by BaCl2 . They can significantly inhibit the incidence rate of ventricular fibrillation induced by chloroform in mice.
Table 1: The Comparison of the types of Ginsenoside between Gingseng Single Decoction and Sheng-Mai-Yin Co-Decoction by HPLC-ESI-MS
HPLC-UV and HPLC-ESI-MS based chemical profiling method was proposed and validated to rapidly evaluate the chemical constituents of Sheng-mai-yin single and co-decoction. Compared with single decoction, the most visible change is that the high and medium-polar ginsenosides in Sheng-mai-yin co-decoction vanished away, such as Notoginsenoside Rg1 , Rb1 , R1 , Rb2 , Ra1 , Ra2 , Ra3 , Rc, Rd and Re. Meanwhile, the chemical compositions of Fructus Schisandrae and Radix Ophiopogoni almost had no change.
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Article Type: Letter to Editor
Citation: Liu JL, Wang XD, Lv CN, Lu JC (2016) The Comparative Study of Quality Between Sheng-MaiYin Single and Co-Decoction. J Pharm Anal Insights 1(2): doi http://dx.doi.org/10.16966/2471-8122.106
Copyright: © 2016 Liu JL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.