Education

• Bachelor of Medicine (MB), Hebei Medical College, China - 1983
• Master of Medicine (MM), Chinese Academy of Medical Sciences/China Union Med. Univ
• Graduate School, Beijing, China - 1986

Academic Training

University MB

• 1978-83    Hebei Medical College, Shi JiaZhuang, Hebei Province, P.R. China
• 1978-80    Basic Medicine
• 1980-82    Clinical Medicine
• 1982-83    Resident Interns

Post Graduate School Master of Medicine-Cancer Pathology

• 1983-1986 - Cancer Institute, Chinese Academy of Medical Sciences, China Union Medical                      University Graduate School, Beijing, China.

Biography

I have been working in the Mallory Institute of Pathology for more than 25 years (started June 1990) as a research associate and then senior scientific staff. Early works were concentrated on the cell biology of colorectal adenomas. I have done culturing and measuring the cell proliferation of adenoma cells both in vitro and in vivo by organ culture and immunohistochemistry. I have been working on the Molecular Pathology for more than 14 years and attended numerous training sessions of PCR and other molecular techniques in research and clinical diagnostic area. As the demand of the department and my research, I have set up the GI research laboratory and the Diagnostic Molecular Pathology Laboratories in 1993 and 1996. I am currently working on the molecular diagnostics of tumors and infectious diseases. Other research areas include breast cancer research. Those involve the detection of AhR and NF-kB expression in animal tissue by immunohistochemistry. My expertise areas are histology, Immunohistochemistry, cell (tissue, organ) culture, molecular biology emphasized on the application of these techniques to clinical diagnostic purposes (i.e. tumor, genetic disease and infectious disease).  During past 25 years I have also involved in a number of teaching opportunities. These include teaching and training of Medical residents in the Department of Pathology, technicians, and fellows for their medical training as well as assisting some of their interested research projects. I have also taught and Co-advised several graduate students on their research projects include MA and PhD students both as co-adviser and thesis defense committee.  Regular teaching include Path PA710 and PA 600/700 courses for consecutive 6 years.

Research Interest

• In-vitro studies of cell proliferation and genetic changes in colorectal adenomas and carcinomas-Serrated Polyp Neoplasia pathway.
• The role of c-myc in breast cancer carcinogenesis.
• Dietary and chemical prevention of cancer in animal models-green tea.
• Molecular Oncology in the field of solid tumors.
• Molecular diagnosis of infectious diseases.
• Lymphoma: Molecular Diagnostic techniques, research and development.
• Molecular and Cellular biology.
• Immunohistochemistry
• Pathology, Diagnostic Molecular
• Carcinogenesis in Breast Cancer and Colorectal Cancer

Scientific Activities

Professional Societies

• 10/1991 -  Member of the American Society for Cell Biology (ASCB)        
• 03/2008 -  Member, Association for Molecular Pathology (AMP-ASIP)
• 05/2009 -  Member, United States and Canadian College of Pathologist (USCAP)

Publications

1. Yang S and Xia QJ, Studies on the Promoting and Initiating Effects of Fusarium T-2 Toxin.  Chung-Hua Ping Li Hsueh Tsa Chih, 17(2): 107-110, 1988. PMID: 3145156          
2. Yang S and Xia QJ, Papilloma of Forestomach Induced by Fusarium T-2 Toxin in Mice.  Chung Hua Chung Liu Tsa Chih, 10(5): 339-341, 1988.PMID: 3248499
3. C Kelly, JC O’Keane, J Orellana, PC Schroy, Shi Yang, JT Lamont and HR Brady; Human Colon Cancer Cells Express ICAM-1 in vivo  and Support LFA-1-Dependent Lymphocyte Adhesion in vitro.  Am. J. Physiol., 263 (Gastrointest. Liver Physiol. 26): G864- 870, 1992. PMID: 1362041
4. P. Schroy, S. Brown-Shimer, K. Kim, K.A. Johnson, M.J. Murnane, S. Yang, M.J. O'Brien, W.C. Carney, H.Z. Kupchik. Detection of p21ras Mutation in Colorectal Adenomas and Carcinomas by  Enzyme-linked Immunosorbent Assay. Cancer 1995; 76:201-9. PMID: 8625092.
5. K Yamaguchi, RA Matulka, AM Shneider, P Toselli, AF Trombino, Shi Yang, LJ Hafer, KK Mann, XJ Tao, JL Tilly, RI Near, and DH Sherr. Induction of PreB Cell Apoptosis by 7, 12-Dimethylbenz(a)anthracene in Long-Term Primary Murine Bone Marrow Cultures. Toxicol. Appl Pharmacol, 147: 190-203, 1997.PMID: 9439715.
6. Adrianne E. Rogers, Laurie J Hafer, Yvette S. Iskander, Shi Yang,  Black Tea and Mammary Gland Carcinogenesis by 7,12-idmethylbenz(a)anhtracene in Rats Fed Control or High Fat Diets. Carcinogenesis, 19 (7): 1269-1273, 1998. PMID: 9683188.
7. Mulligan NJ, Yang S, Andry C, Klein M, O’Brien MJ. The Role of p21ras in Pancreatic Neoplasia and Chronic Pancreatitis. Human Pathol. June, vol. 30(6): 602-610, 1999. PMID: 10374765.
8. Rodolfo Robles, Yutaka Morita, Koren K. Mann, Gloria I. Perez, Shi Yang, Tiina Matikainen, David H. Sherr and Jonathan L. Tilly.  The Aryl Hydrocarbon Receptor, a Basic Helix-loop-helix Transcription Factor of the PAS Gene Family, is Required for Normal Ovarian Germ Cell Dynamics in the Mouse.  . Endocrinology: 141(1): 450-453, 2000. PMID: 10614669.
9. Trombino AF1, Near RI, Matulka RA, Yang S, Hafer LJ, Toselli PA, Kim DW, Rogers AE, Sonenshein GE, Sherr DH. Expression of the aryl hydrocarbon receptor/transcription factor (AhR)           and AhR-regulated CYP1 gene transcripts in a rat model of mammary tumorigenesis. Breast Cancer         Res Treat. 2000 Sep;63(2):117-31. PMID: 11097088.
10. MJ O'Brien, S Yang, JL Clebanoff, E Mulcahy, FA Farraye, M Amorosino, N Swan. Hyperplastic (serrated) Polyps of the Colorectal: Relationship of CpG - Island Methylation Phenotype and K-ras Mutation to Location and Histologic Subtype. Am J Surg Pathol. 28(4): 423-434, 2004. PMID: 15087661.
11. Christopher S Huang, Michael J O'Brien, Shi Yang and Francis A Farraye. Hyperplastic polyps, Serrated Adenomas and the Serrated Neoplasia Pathway. Am J Gastroenterol. 99: 2242-2255, 2004. PMID: 15555008.
12. Shi Yang, Francis A. Farraye, Charline Mack, Oksana Posnik and Michael J. O'Brien. BRAF and KRAS Mutations in Hyperplastic Polyps and Serrated Adenomas of the Colorectum. Am J Surg Pathol. 28(11): 1452-1459, 2004.PMID: 15489648.
13. Stephen A Murray, Shi Yang, Elizabeth Demicco, Haoqiang Ying, David H Sherr, Laurie J Hafer, Adrianne E Rogers, Gail Sonenshein and ZhiXiong Jim Xiao. Increased expression of MDM2, cyclin D1, and p27Kip1 in carcinogen-induced rat mammary tumors. J Cell Biochem. 2005 Aug.1;95(5):875-84.PMID: 15844214.
14. Michael J O’Brien, Shi Yang,  Charline Mack, Huihong Xu, Christopher S Huang, Elizabeth Mulcahy, Mark Amorosino, Francis A Farraye.  Comparison of Microsatellite Instability, CpG Island Methylation Phenotype, BRAF and KRAS Status in Serrated Polyps and Traditional Adenomas Indicates Separate Pathways to Distinct Colorectal Carcinoma Endpoints. AJSP 2006;30:1491-1501.PMID: 17122504.
15. Andrey A. Sharov, Tatyana Y. Sharova, Andrei N. Mardaryev, Alice Tommasi di Vignano,Ruzanna Atoyan, Lorin Weiner, Shi Yang, Janice L. Brissette, G. Paolo Dotto, and Vladimir A. Botchkarev. Bone morphogenetic protein signaling regulates the size of hair follicles and modulates the expression of cell cycle-associated genes. Proc Natl Acad Sci U S A. 2006; 103(48): 18166-18171. PMID: 17114283.
16. Daniel W. Rosenberg, Shi Yang, Devon C. Pleau, Emily J. Greenspan, Richard G. Stevens, Thiruchandurai V. Rajan, Christopher D. Heinen, Joel Levine, Yijian Zhou, and Michael J. O'Brien.  Mutations in BRAF and KRAS Differentially Distinguish Serrated versus Non-Serrated Hyperplastic Aberrant Crypt Foci in Humans. Cancer Res 2007; 67(8): 3551-3554. PMID: 17440063.
17. Karine Belguise, Shangqin Guo, Shi Yang, Adrianne E. Rogers, David C. Seldin, David H. Sherr, and Gail E. Sonenshein. Green Tea Polyphenols Reverse Cooperation between c-Rel and CK2 that Induces the Aryl Hydrocarbon Receptor, Slug, and an Invasive Phenotype. Cancer Res 2007;67(24):11742-50. PMID: 18089804.
18. Michael J O’Brien, Shi Yang, Christopher S Huang, Courtney Shepherd, Sandra Cerda,  Francis A Farraye. The Serrated Polyp Pathway to Colorectal Carcinoma. Diagnostic Histopathology. 2008;14(2):78-93. NO PMID.
19. Vesela Kovacheva, Jessica Davison, Tiffany Mellott, Adrianne Rogers, Shi Yang, Michael O'Brien and Jan Blusztajn. Raising Gestational Choline Intake Alters Gene Expression in DMBA-evoked Mammary Tumors and Prolongs Survival. The FASEB Journal 2008 Dec. 1(Epub ahead of print).PMID: 19047067.
20. O.E. Dadzie, S. Yang, A. Emley, M. Keady, J. Bhawan and M. Mahalingam. RAS and RAF mutations in banal melanocytic aggregates contiguous with primary cutaneous melanoma: clues to melanomagenesis. BJD, 2009;160:368-375. PMID: 18945298.
21. JC Lee, S Yang, Y Zou and L Joseph. Erythroid/B-cell biphenotypic acute leukemia first case report. Leukemia 2009. Leukemia advance online publication, 21 May 2009; doi:10.1038/leu.2009.105. PMID: 19458630.
22. Jeremy T. Hetzel, Christopher S. Huang, Jennifer A. Coukos, Kelsey Omstead, Sandra R.  Cerda, Shi Yang, Michael J. O’Brien, Francis A. Farraye.  Variation in the detection of serrated polyps in an average risk colorectal cancer screening cohort.  Am J Gastroenterol 2010; 105:2656–2664. PMID: 20717107.
23. Emley A, Yang S, Wajapeyee N, Green MR, Mahalingam M. Oncogenic BRAF and the tumor    suppressor IGFBP7 in the genesis of atypical spitzoid nevomelanocytic proliferations. J Cutan Pathol.  2010 Mar; 37(3):344-9. PMID: 19788444.
24. Andrew Emley, Lisa Nguyen,  Shi Yang, Meera Mahalingam. Somatic mutations in GNAQ in amelanotic/hypomelanotic blue nevi. (Human Pathology 2010 Nov. 5, e-pub). PMID: 21055890.
25. Christopher S. Huang, Francis A. Farraye, Shi Yang, Michael J. O’Brien. The Clinical  Significance of Serrated Polyps. Am J Gastroenterol advance online publication, 2 November 2010; doi: 10.1038/ajg.2010.429. PMID: 21045813.
26. DeCarlo K, Yang S, Emley A, Wajapeyee N, Gree n M, Mahalingam M. Oncogenic BRAF positive dysplastic nevi and the tumor suppressor IGFBP7– challenging the concept of dysplastic nevi as precursor lesions? Hum Pathol. 2010;41:886. PMID: 20233623.
27. Brahmbhatt M, Yang S, Mahalingam M (December 2010). Proliferative Nodules in Congenital Nevi - A Histopathologic, Genetic and Immunohistochemical Reappraisal. J Clin Exp Dermatol Res 1:105. doi:10.4172/2155-9554.1000105 PMID: 25893138.
28. Elizabeth Park, Shi Yang, Andrew Emley, Kristen Decarlo, Joanna Richards and Meera Mahalingam. Lack of correlation between immunohistochemical expression of CKIT and KIT mutations in atypical acral nevi. Am J Dermatopathol. 2012 Feb;34(1):41-6. PMID#: 22094233.
29. Michael H. A. Roehrl, Marier Hernandez, Shi Yang, Thomas G. Christensen, Claudio Morera, and Julia Y. Wang “Helicobacter Heilmannii Gastritis in a Young Patient with Pets” Gastrointestinal Endoscopy 2012 Aug;76(2):421-2. doi: 10.1016/j.gie.2012.03.1403. PMID: 22817795.
30. Miller DD, Emley A, Yang S, Richards JE, Lee JE, Deng A, Hoang MP, Mahalingam M. Mixed versus pure variants of desmoplastic melanoma: a genetic and immunohistochemical appraisal. Mod Pathol. 2011 Dec 9. doi: 10.1038/modpathol.2011.196. [Epub ahead of print] PMID: 22157936.
31. Feller K, Yang S, Tung N, Lee J, Mahalingam M. c-myc in Kaposi's sarcoma: analyses by fluorescent in situ hybridization and immunohistochemistry. J Eur Acad Dermatol Venereol. 2012 Aug 7. doi: 10.1111/j.1468-3083.2012.04672.x. [Epub ahead of print].  
32. Feller JK, Yang S, Mahalingam M. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma. Mod Pathol. 2013 Mar;26(3):414-20. doi: 10.1038/modpathol.2012.168. Epub 2012 Oct 5. PMID:23041829.
33. Aung PP, Batrani M, Yang S, Mahalingam M. Apoptosis and In Situ and Invasive Squamous Cell Carcinoma in Sun-Exposed Sites. Am J Dermatopathol. 2013 Feb 28. [Epub ahead of print] PMID: 23455334
34. Drew DA, Devers T, Horelik N, Yang S, O'Brien M, Wu R, Rosenberg DW. Nanoproteomic analysis of extracellular receptor kinase-1/2 post-translational activation in microdissected human hyperplastic colon lesions. Proteomics. 2013 May;13(9):1428-36. doi:10.1002/pmic.201200430. Epub 2013 Apr 4. PMID: 23467982
35. Yingshe Zhao, Joerg Kumbrink, Bor-Tyh Lin, Amy H. Bouton, Shi Yang, Paul A. Toselli1  and Kathrin H. Kirsch1 Expression of a Phosphorylated Substrate Domain of p130Cas  Promotes PyMT-induced c-Src-dependent Murine Breast Cancer Progression. Carcinogenesis. 2013 Aug 16. [Epub ahead of print]  PMID: 23825155.
36. Mitchell B, Leone D, Feller JK, Menon S, Bondzie P, Yang S, Park HY, Mahalingam M Protein expression of the chemokine receptor CXCR4 and its ligand CXCL12 in primary cutaneous melanoma—biomarkers of potential utility? Hum Pathol. 2014 Oct;45(10):2094-100. doi: 10.1016/j.humpath.2014.06.018. Epub 2014 Jul 2. PMID: 25130395, IF=2.806
37. Susan B. Brogly, PhD, MSc, Rebecca B. Perkins, MD, MSc, Dimity Zepf, BS, Janina Longtine, MD and Shi Yang, MD, MSc.  Human Papillomavirus Vaccination and Cervical Cytology in Young Minority Women. Sexually Transmitted Diseases.2014;41(8):511-514. PMID:25013981. IF=2.748.
38. Aung PP, Leone D, Feller JK, Yang S, Hernandez M, Yaar R, Singh R, Helm T, Mahalingam M.  Microvessel density, lymphovascular density and lymphovascular invasion in primary cutaneous Melanoma. Human Path. 2014 Nov 26. pii: S0046-8177(14)00475-4. doi:    10.1016/j.humpath.2014.11.006. [Epub ahead of print] PMID: 25537974. IF=2.806.
39. O’Brien MJ, Zhao Q and Yang S. Colorectal Serrated Pathway Cancers and Precursors. Histopathology. 2014 Sep 29. doi: 10.1111/his.12564. [Epub ahead of print].    PMID:25263173.IF=3.301
40. Mitchell B, Yang S, Bondzie P, Feller KJ, Park HY, Leone D, Mahalingam M.  Correlation of  chemokine receptor CXCR4 mRNA in primary cutaneous melanoma with established histopathologic prognosticators and the BRAF status. Melanoma Research 2014 Dec;24(6):621-5. doi: 10.1097/CMR.0000000000000120. PMID:25211166[PubMed - in process]  IF=2.098.
41. Wu H, Sun Y, Ye H, Yang S, Lee SL, de Las Morenas A. Anaplastic Thyroid Cancer: Outcome and the Mutation/Expression Profiles of Potential Targets. Pathol Oncol Res. 2015 Jan 15. [Epub ahead of print], PMID:25588542 [PubMed - as supplied by publisher]. IF=1.806
42. Noah Frydenlund, Dominick Leone, Brendon Mitchell, Shi Yang, April Deng, Mai P Hoang and              Meera Mahalingam.  Neurotrophin receptors and perineural invasion in desmoplastic melanoma –            correlation with histopathologic variables. J Am Aca Derm. 2015 Mar 7. pii: S0190-9622(15)00091-doi:10.1016/j.jaad.2015.01.026. [Epub ahead of print] PMID:25752716 [PubMed - as supplied by publisher] IF=5.004.
43. Hye Jin Chung, MD, MMS, Shi Yang, MD, MSc, Farah Succaria, MD, Jag Bhawan, MD.    Research letter: TERT promoter mutations are infrequent in Merkel cell carcinomas. J Am Acad Dermatol. 2015 Nov; 73(5):864-5. PMID: 26475538 ,  IF=4.449
44. Brendon Mitchell, Dominick Leone, Shi Yang, Ashraf Khan, Jagdish Dhingra and Meera Mahalingam. BRAF and Epithelial-Mesenchymal Transition in Primary Cutaneous Melanoma: A Role for Snail and E-Cadherin?" Hum Pathol. 2016 Jun;52:19-27. doi:    10.1016/j.humpath.2015.12.030. Epub 2016 Feb 1.PMID: 26980024 IF=2.769
45. Alexander Kadokura, BS,1 Noah Frydenlund1, MS,2 Dominick A. Leone, MPH,3 Shi Yang, MD,4  Mai P. Hoang, MD, PhD,5 April Deng, MD, PhD,6 Marier Hernandez-Perez, MD,7 Asok Biswas, MD, FRCPath, DipRCPath,8 Rajendra Singh, MD, 9 Ron Yaar, MD, PhD, 10 and Meera Mahalingam, MD, PhD, FRCPath11Neurofibromin protein loss in desmoplastic melanoma subtypes: implicating NF1 allelic loss as a distinct genetic driver?  Hum Pathol. 2016 Jul; 53:82-90. PMID: 26980030.
46. Shi Yang, MD1* , Dominick A. Leone, MPH2*, Alexander Kadokura, BS3, Noah Frydenlund, MS4, Mai P. Hoang, MD5, April Deng, MD, PhD6, Marier Hernandez-Perez, MD7, Asok Biswas, MD, FRCPath, DipRCPath8, Rajendra Singh, MD 9, Ron Yaar, MD, PhD 10 and Meera Mahalingam, MD, PhD, FRCPath11 Frequency of TERT promoter mutations in desmoplastic melanoma subtypes: analyses of 76 cases. Melanoma Research, May 2016. IF=2.28. PMID: 27244099.
47. Mitchell B, Leone D, Yang S, Khan A, Mahalingam M, Dhingra J.    BRAF and epithelial mesenchymal transition in papillary thyroid carcinoma - challenging the roles of Snail and E-Cadherin?  Am J Transl Res. 2016 Nov 15;8(11):5076-5086. eCollection 2016. PMID: 27904709, IF: 3.146.
48. Noah Frydenlund, Dominick Leone, Shi Yang, Mai Hoang, April Deng, Marier Hernandez-Perez,   Rajendra Singh, Asok Biswas, Ron Yaar, Meera Mahalingam.   Tumoral PD-L1 expression in desmoplastic melanoma is associated with depth of invasion, tumor infiltrating CD8 cytotoxic lymphocytes and the mixed cytomorphologic variant. Mod Pathol. 2017 Mar;30(3):357-369. doi:   10.1038/modpathol.2016.210. Epub 2017 Jan 13. PMID:  28084337.
49. Josenia Tan, Antonio de las Morenas , Shi Yang and Huihong Xu. Comparison of the        clinicopathologic features of solitary and multifocal papillary thyroid carcinoma and the utility of testing an additional tumor nodule for BRAFV600E in multifocal cases. J Mod Hum Pathol. 2017, 2(1):1-6  http://nobleresearch.org/Doi/10.14312/2397-6845.2017-1. PMID: xxxxxxxx, IF: None.
50. Mahmoud L. Soliman, Shi Yang, John Cho Lee. The utility of KRAS mutation analysis in differentiating a lung primary mucinous carcinoma from a metastatic colorectal mucinous       carcinoma. Case Reports in Clinical Pathology: vol 4(4), 2017. Online publication, Dec 12, 2017. 
51. Yuriy O. Alekseyev, Roghayeh Fazeli, Shi Yang, Raveen Basran, Thomas Maher, Nancy S. Miller           and Daniel Remick.  A Next Generation Sequencing Primer – How Does It Work and What Can It            Do? Accepted for Publication  2/11/18, Academic Pathology. IF= (none).
52. Ana de la Cueva, Michael J. Emmerling, Sara J. Lim, Shi Yang, Philip C. Trackman, Gail E.                    Sonenshein and Kathrin H. Kirsch.  A Polymorphism in the Lysyl Oxidase Propeptide Domain                 Accelerates Carcinogen induced Cancer. Accepted for publication by Carcinogenesis. 3/11/2018. IF:         5.105 (2017).