D. Michael Olive
D. Michael Olive
Adjunct Professor of Biology

Nebraska Wesleyan University


  • Postdoctoral Researcher, University of Illinois at the Medical Center
  • Ph.D. (Medical Microbiology); Loyola University Stritch School of Medicine, Chicago, IL, USA
  • BA (Zoology); DePauw University, Greencastle, IN, USA



Corporate research and product development executive with twenty years experience in senior management. Co-inventor of the Invader Assay, a nucleic acid amplification technology that was commercialized and valued at over $500 million. Broad experience in medical microbiology, molecular biology, oncology, cell signaling, and diagnostic devices. Deep knowledge of molecular imaging, contrast agents, and translation of imaging agents to clinical use, including maneuvering through regulatory filings.

Research Interest

My research focuses on developing fluorescence contrast agents and instrumentation for fluorescence-guided cancer surgery. The hope is that these agents will enable more efficient surgical removal of solid tumor tissue, resulting in better therapeutic outcomes.

Professional Activities:

2015 Present Adjunct Professor Biology Nebraska Wesleyan University
2012-Present Principal Olive Consulting
2009-20012 Vice President Translational Research LI-COR Biosciences
1999-2009 Vice President Science & Technology LI-COR Biosciences
1997-1999 Vice president Business Development Applied Spectral Imaging
1995-1997 Vice president & Chief Technical Officer Third Wave Technologies
1990-1995 Director Infectious Disease & Assay Integration GENE-TRAK/Vysis
1984-1990 Associate Professor Kuwait University Medical Center
1984-1990 Director WHO Polio Virus Eradication program
1981-1984 Senior Molecular Biologist Pharmacia P-L Biochemicals



  1. Olive, D.M.,Lampert, M., and Major, E.O. 1980. Comparison of wild type BK virus and BK virion DNA rescued from virus transformed BHK cells.  Virology 103:1-10.
  2. Bergsma, D.J., Olive, D.M., Hartzell, S.W., and Subramanian, K.N. 1982. Territorial limits and functional anatomy of the simian virus 40 replication origin.  Proc. Natl. Acad. Sci. USA. 79:381-85.
  3. Bergsma, D.J., Olive, D.M., Hartzell, S.W., Byrne, B.J., and Subramanian, K.N.  1982.  Cyclization of linear chimeric plasmids in vivo by a novel end to end joining reaction or by intramolecular recombination: One of the products contains a 147 bp perfect palindrome stable in Escherichia coli.  Gene 20:157-167.
  4. Olive, D.M., Atta, A.I., and Sethi, S.K. 1988 Detection of toxigenic Escherichia coliusing biotin labeled DNA probes following enzymatic amplification of the heat labile toxin gene.  Mol. Cell. Probes.  2:47-57.
  5. Olive, D.M., El-Mekki, A., Al-Mulla, W., Khalik, D.A., and Al-Nakib, W. 1988. The use of ELISA and non-radioactive DNA hybridization assays for the detection of human cytomegalovirus. J. Virol. Meth.  19:289-298.
  6. Olive, D.M.,Khalik, D.A., and Sethi, S.K. 1988. Identification of enterotoxigenic Escherichia coli using alkaline phosphatase-labeled synthetic oligonucleotide probes.  Eur. J. Clin. Microbiol. Infect. Dis. 7:167-171.
  7. Sethi, S.K., Olive, D.M.,Strannegard, O.O., and Al-Nakib, W. 1988. Molecular epidemiology of human rotavirus infections based on genome segment variations in viral strains.  J. Med. Virol. 26:249-259.
  8. Olive, D.M. and Sethi, S.K. 1989. Detection of human rotavirus using an alkaline phosphatase-conjugated synthetic DNA probe in comparison with enzyme-linked immunoassay and polyacrylamide gel analysis.  J. Clin. Microbiol.  27:53-57.
  9. Olive, D.M. 1989. Detection of enterotoxigenic Escherichia coli following polymerase chain reaction amplification using a thermostable DNA polymerase.  J. Clin. Microbiol. 27:261-265.
  10. Olive, D.M., Simsek, M., and Al-Mufti, S. 1989. Polymerase chain reaction assay for the detection of human cytomegalovirus.  J. Clin. Microbiol. 27:1238-1242.
  11. Olive, D.M., Al-Mufti, S., Simsek, M., and Al-Nakib, W. 1989. Direct detection of human cytomegalovirus in urine specimens following polymerase chain reaction amplification.  J. Med. Virol. 29:232-237.
  12. Olive, D.M.,Johny, M., and Sethi, S.K. 1990. Detection of Campylobacter jejeuni and Campylobactercoli using an alkaline phosphatase-labeled synthetic oligonucleotide probe.  J. Clin. Microbiol. 28:1565-1569.
  13. Olive, D.M., Al-Mulla, W., Simsek, M., Zarban, S., and Al-Nakib, W. 1990. The human cytomegalovirus immediate early enhancer-promoter is responsive to activation by the adenovirus-5 13S E1a gene.  Arch. Virol. 112:67-80.
  14. Olive, D.M., Al-Mufti, S., Al-Mulla, W., Pasca, A., Khan, M., Stanway, G., and Al-Nakib, W. 1990.  Detection and differentiation of picornaviruses in clinical samples following genomic amplification.  J. Gen. Virol. 71:2141-2147.
  15. Simsek, M., Olive, D. M., and Al-Hassan, J.M. 1990.  Analysis of mitochondrial DNA by restriction endonucleases to distinguish three species of Ariid catfish from the Arabian Gulf.Biochem. Syst. Ecol.  18:467-471.
  16. An, Q., Radcliffe, G. R., Vassallo, R., Buxton, D., O'Brien, W. J., Pelletier, D., Weisburg, W. G., Klinger, J., and Olive, D. M. 1992. Infection with a plasmid-free variant chlamydia related to Chlamydia trachomatis identified using multiple assays for nucleic acid detection.  J. Clin. Microbiol. 30:2814-2821.
  17. An, Q and Olive, D. M. 1994. Molecular cloning and nucleic acid sequencing of the Chlamydia trachomatis 16S rRNA genes from patient specimens lacking the cryptic plasmid.   Mol. Cell. Probes.  8:429-435.
  18. Shah, J., Lui, J., Smith, J., Popoff, Radcliffe, G., O. Brien, W. J., S., Serpe, E., Olive, D. M., and King, W. 1994.  Novel, ultrasensitive Q-beta replicase-amplified assay for detection of Chlamydia trachomatis.  J. Clin. Microbiol.  32:2718-2724.
  19. An, Q., Lui, J., Shah, J., King, W., Popoff, S., O'Brien, W., Buxton, D., and Olive, D. M. 1994. Comparison of Q-replicase amplified assay with a competitive PCR assay for Chlamydia trachomatis.   J. Clin. Microbiol.  33:58-63.
  20. Shah, J., Lui, J., Popoff, S., Serpe, E., King, W., An, Q., Olive, D. M., Mahan, D., and Klinger, J. 1994. Detection of Mycobacterium tuberculosis directly from spiked human sputum by Q-replicase amplified assay.  J. Clin. Microbiol. 33:322-328.
  21. An, Q., Buxton, D.,, A. Hendricks, L. Robinson, J. Shah, L. Lu, M. Vera-Garcia, W. King, and Olive,D. M. 1994. Comparison of amplified Qreplicase and PCR assays for detection of Mycobacterium tuberculosis. J. Clin. Microbiol. 33:860-867.
  22. Shah, J., Lui, J., Popoff, S., Serpe, E., King, W., An, Q., Olive, D. M., Mahan, D., and Klinger, J. 1994. Clinical performance of a Q-replicase amplified assay in detecting Mycobacteria tuberculosis in human sputum specimens. J. Clin. Microbiol. 33:1435-1441.
  23. Brow, M.A.D., M. C. Oldenburg, V. Lyamichev, L. M. Heisler, J. G. Hall, N. J. Eagan, D. M. Olive, L. M. Smith, L. Fors, and J. E. Dahlberg. 1996. Differentiation of 16S rRNA genes, intergenic regions, and Mycobacterium tuberculosis katG genes by structure-specific endonuclease cleavage.  J. Clin. Microbiol. 34:3129-3137.
  24. Smith, J. H., D. Buxton, P. Cahill, M. Fiandaca, L. Goldston, L. Marselle, S. Rigby, M. Olive, A. Hendricks, T. Shimei, J. D. Klinger, D. J. Lane, and D. E. Mahan. 1997. Detection of Mycobacterium tuberculosis directly from sputum using a prototype automated Q-beta replicase assay.  J. Clin. Microbiol. 35:1477-1483.
  25. Marshall, D. J., L. M. Heisler, V. Lyamichev, C. Murvine, D. M. Olive, G. D. Ehrlich, B. P. Neri, and M. de Arruda. 1997. Determination of hepatitis C virus genotypes in the United States by Cleavase Fragment Length Polymorphism analysis. J. Clin. Microbiol. 35:3156-3162.
  26. Olive, D. M. and P. Bean. 1999. Principles and applications of DNA-based typing of microbial organisms.  J ClinMicrobiol. 37:1661-1669.
  27. Olive. D. M., A. Geschwender, H. Chen, M. Urh, M. Esser, and Y. Zhang. 2002. The application of infrared fluorescent technology for the analysis of proteins and nucleic acids. In Biomedical Nanotechnology Architectures and Applications, Proceedings of SPIE.  Eds.D. J. Bornhop.   4626: 170-183.
  28. Calvert, V.S., Yihui-Tang, E., Boveia, V., Wulfkuhle, J., Schutz-Geschwender, A., Olive, D.M.,Liotta, L.A., and Petricoin, E.F. 2004. Development of multiplexed signal transduction pathway profiling and detection using near infra-red detection of reverse phase protein microarrays.  Clinical Proteomics Journal. 1:81-89.
  29. Olive, D. M. 2004. Quantitative methods for analysis of protein phosphorylation in drug development.  Expert Rev. Drug. Discovery.  1:327-341.
  30. Chen, H., Kovar, J., Sissons, S., Cox, K., Matter, B., Chadwell, F., Luan, P., Vlahos, C.J., Schutz-Geschwender, A., and Olive, D.M. 2005. A cell based immunocytochemical assay for monitoring kinase signaling pathways and drug efficacy.  Anal. Biochem. 338:136-142.
  31. Kovar, J.L., Simpson, M.A., Schutz-Geschwender, A., and Olive, D.M. 2007. A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models.  Anal. Biochem. 367:1-12.
  32. Weldon, S., Ambroz, K, Schutz-Geschwender, A., and Olive, D. M. 2008. Near-infrared fluorescence detection permits accurate imaging of loading controls for Western blot analysis Anal. Biochem. 375:156-158.
  33. Ambroz, K.L.H., Zhang, Y., Schutz-Geschwender, A., and Olive, D.M. 2008. Blocking and detection chemistries affect antibody performance on reverse phase protein arrays. Proteomics. 8:2379-2383.
  34. Kovar, J.L., Volcheck, W.A., Sevick-Muraca, E. M., Simpson, M.A., and Olive, D. M. 2009.  Characterization and performance of a near infrared 2-deoxyglucose optical imaging agent for mouse cancer models.  Anal Biochem 384: 254-262
  35. Gong, H., Zhang, B., Little, G., Kovar, J., Chen, H.,Xie, W., Schutz-Geschwender, A., and Olive,D.M. 2009. β-galactosidase activity assay using far red-shifted fluorescent substrate DDAOG. Anal. Biochem. 386:59-64.
  36. Peng, X., Chen, H., Draney, D. R., Schutz-Geschwender, A., and Olive, D. M. 2009. A non-fluorescent, broad range quencher dye for FRET assays. Anal. Biochem. 388:220-228.
  37. Gong, H., Kovar, J., Little, G., and Olive, D.M. 2009. Near-infrared labeled affibody probes for in vivo detection of tumors over expressing EGFR and Her2. Neoplasia. 12:139-149.
  38. Marshall, M., Draney, D., Sevick-Muraca, E.M., and Olive, D.M. 2010. Single Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley Rats.Mol. Imaging Biol. 12:583-594.
  39. Gong, H., Little, G., Cradduck, C., Draney, D.R., Padhye, N., and  Olive, D.M. 2011. Alkaline phosphatase assay using a near-infrared fluorescent substrate merocyanine 700 phosphate. Talanta 84: 941–946.
  40. Kovar, J.L., Xu, X., Draney, D., Cupp, A., Simpson, M.A. and Olive, M.A. 2011. Near infrared labeled tetracycline derivative is an effective marker of bone deposition in mice. Anal. Biochem, 416:167-173.
  41.  Gong, H., Kovar, J.L., Baker, B., Zhang, A., Cheung, L., Draney, D.R., Correa, I.R. Jr.,Xu, M-Q., Olive, D.M. 2012. Near-Infrared Fluorescence Imaging of Mammalian Cells and Xenograft Tumors with SNAP-Tag. 2012. PLoS ONE 7:1-8.
  42. Huang, R., Vider, J., Kovar, J.L., Olive, D.M.,Mellinghoff, I.K.,Mayer-Kuckuk, P., Kircher, M.F., and Blasberg, R.G. 2012. Integrin v3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma. Clin. Cancer Res. 18:5731-5740.
  43. Kovar, J.L., Curtis, E., Othman, S, Simpson, M.A., and Olive, D.M. 2013. Characterization of a near infrared-labeled chlorotoxin imaging agent for detection of spontaneous medulloblastomas in ND2:SmoA1 mice. Anal. Biochem. 440:212-219.
  44. vanOosten, M., Schäfer, T., Gazendam, J., Ohlsen, K., Tsompanidou, E., de Goffau, M.C., Harmsen, H.J.M., Crane, L., Francis, K., Cheung, L., Olive, D.M., Ntziachristos, V., van Dijl, J.M., and van Dam, G.M. 2013. Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labeled vancomycin. Nature Comm. DOI: 10.1038/ncomms3584: 1-8.
  45. Gong, H., Kovar, J. L., Cheung, L.L., Rosenthal, E.L., and Olive, D.M. 2014. A comparative study of affibody, panitumumab, and EGF for near-infrared fluorescence imaging of EGFR- and EGFRvIII- expressing tumors. Cancer Biol. Ther. 15:185-193.
  46. Kovar, J.L., Cheung, L.L., Simpson, M.A., Olive, D.M. 2014.  Pharmacokinetic and biodistribution assessment of a near infrared-labeled PSMA-specific small molecule in tumor-bearing mice.Prostate Cancer. 2014: http://dx.doi.org/10.1155/2014/104248.

Publications In Books

  1. Piatek, M., Olive, D.M., Subramanian, K.N., Ghosh, P.K., Reddy, V.B., Lebowitz, P., and Weissman, S.M.  1980.  The structure and expression of the late region of SV40 DNA.  In “Gene Structure and Expression”.  Eds. Dean, D.H., Johnson, L.F., Kimble, P.C., and Perlman, P.S.  Ohio State University Press.  Columbus, Ohio. pp 153-173.
  2. Olive, D. M. Q-Beta replicase assay for the clinical detection of infectious agents. 1997. In, Nucleic Acid Amplification Technologies. Application to Disease Diagnosis.  Eds. Helen Lee, Stephen A. Morse and OrjianOlsvik.  Eaton Publishing, Boston, MA.pp. 101-112.
  3. Gong, H., Sampath, L., Kovar, J. L., and Olive, D. M. 2012. Targeting EGFR and Her2 for molecular imaging of cancer. In Molecular Imaging. Ed. Shaller, B. pp. 351-374.




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