shih-chen
Arathy DS Nair, PhD
Research Associate of Diagnostic Medicine and Pathobiology

Kansas State University
Manhattan, Kansas, USA
Email: arathynirazhi@gmail.com; arathy@vet.k-state.edu


Education

2010

PhD Biotechnology

Rajiv Gandhi Centre for Biotechnology, India

2003

MVSc, Animal Physiology

Indian Veterinary Research Institute, India

 

Biography

 

 

Research Interest

Immunology

Solid phase and liquid phase blocking ELISA, Western Blot, Antibody Raising, Purification and Characterization, Virus Neutralization Test, Serum Neutralization Test.

Molecular Biology

Plasmid Isolation, Isolation of DNA from Plant, Animal and Bacteria Sources, RNA Isolation, Restriction Analysis, Conventional and Real-time PCR, RT-PCR, Amplification, Primer Design, Automated Sequencing, Cloning and Expression, Protein Purification (Affinity Chromatography), Competent Cell Preparation and Transformation, Agarose Gel Electrophoresis and Poly acrylamide Gel Electrophoresis

Animal Models and Experimentation

Immunization, Small and Large Animal Surgery

Cell Culture Techniques

Cell Line Maintenance (BHK-21, VERO, MDBK, HEK), Primary Chicken EmbryoFibroblast Culture, Propagation of Viruses in Embryonated Eggs and Cell Culture.

Computational Biology

DNA and Protein Sequence Analysis (Phylip, MEGA, DNA STAR package, MFOLD and Bioedit)

 

Professional Activities

Awards & Honors

2013

ACVM board eligible.
Travel award to attend the 26th ASR Meeting held in Portland, ME from June 15-18

2012

Travel award to attend the 25th ASR Meeting to be held in Park City, UT from July 28-31

2009

Selected as Assistant Professor in the division of Physiology, College of Veterinary and Animal Sciences, Thrissur, India

2008

Best poster award- 10thAvian Immunology Research Group Meeting held at Gold cost, Brisbane, Australia.
Travel grant to participate in The Bangkok International Conference on Avian Influenza

2005

Indian Council of Agricultural Research- National Eligibility Test

2004

Best poster presentation award –Indian Society for Veterinary Immunology and Biotechnology, Mukteswar, India.

2003

Young Scientist Award Recipient,Indian Society for Study of Animal Reproduction (2003), Kolkata

Professional Experience

2011-Present

KANSAS STATE UNIVERSITY, College of Veterinary Medicine,Manhattan, KS, USA

2009-2011

ST. GEORGE'S UNIVERSITY, School of Veterinary Medicine, Grenada, West Indies

2005-2010

RAJIV GANDHI CENTR FOR BIOTECHNOLOGY, TRIVANDRUM, INDIA

Professional affiliation
  1. Member- American Society for Rickettsiology
  2. Life Member- Indian Society for Study on Animal Reproduction (ISSAR)
  3. Life Member- Indian Society of Veterinary Immunology and Biotechnology
  4. Life member – Kerala Veterinary Council.

 

Publications

Publications and Presentations
  1. Comparative experimental infection study in dogs with five tick-borne Anaplasmataceae pathogens; Ehrlichiacanis, E. chaffeensis, E. ewingii, Anaplasmaphagocytophilumand
    A. platys.
    Arathy D. S. Nair, Chuanmin Cheng, Chanran K. Ganta, Katherine A Sayler, Arthur R. Alleman, Ulrike G. Munderloh and Roman R. Ganta (Manuscript under preparation).
  2. Ehrlichiachaffeensis Infection in the Reservoir Host (White-tailed Deer) and in an Incidental Host (Dog) Is Impacted By Its Prior Growth in Macrophage and Tick Cell EnvironmentsArathy D. S. Nair, Chuanmin Cheng, Deborah C. Jaworski, Lloyd Willard, Michael W Sanderson and Roman R. Ganta (Manuscript accepted for publication inPlos One)
  3. Attenuated Mutants of Ehrlichiachaffeensisin Inducing Protection against Wild-type Infection Challenge. Chuanmin Cheng, Arathy D. S. Nair*, Deborah C. Jaworski, SuhasiniGanta, and Roman R. Ganta (* Co-first author with equal contribution).
  4. Real-time PCR Assay Validation for Detecting Rickettsia rickettsii infections in dogs and ticks. Arathy DS Nair, NilimaBhoi, Ram Raghavan, and Roman R Ganta. (Manuscript under preparation)
  5. Evaluation of Dermacentorvariabilis for the incidence of pathogens causing diseases in animals and humans. NilimaBhoi, Arathy DS Nair, Gary A Anderson, Roman R Ganta, and Ram K Raghavan. (Manuscript under preparation)
  6. Targeted and Random Mutagenesis of Ehrlichiachaffeensis for the Identification of Genes Required for In vivo Infection. Cheng C, Nair ADS, Indukuri VV, Gong S, Felsheim RF, et al. (2013) PLoSPathog 9(2): e1003171. doi:10.1371/journal.ppat.1003171.
  7. Antimicrobial drug resistance in Escherichia coli isolated from commercial chicken eggs in Grenada, West Indies. (2010)  D S Arathy, G Vanpee, G Belot, V Mathew, C DeAllie and R Sharma. West Indian Med J. 2011 Jan; 60(1):53-6.
  8. Bacterial contamination of commercial chicken eggs in Grenada, West Indies. (2009)   D S Arathy, G Vanpee, G Belot, V Mathew, C DeAllie and R Sharma. West Indian Veterinary Journal 2009, 9 (2) 4-7.
  9. Preliminary molecular characterization of a fowlpox virus isolate in Grenada (2010).  Arathy, D.S., Tripathy, D.N., Sabarinath, G.P., Bhaiyat, M.I., Chikweto, A., Matthew, V. and Sharma, R.N. Avian Diseases 54(3):1081-1085.
  10. Antimicrobial resistance and phylogenetic groups of commensal Escherichia coli from healthy pigs in Grenada, ArathySabarinath, Keshaw Tiwari, Claude DeAlliec, Guillaume Belot, Guillaume Vanpee, Vanessa Matthew, Graeme Stratton, Ravindra Sharma and Harry Hariharan (published on line on WebmedCentral.com)
  11. Doxycyclne treatment of asymptomatic dogs seropositive for Ehrlichiacanis. M. Lanza-Perea; S. Kumthekar, A. Sabarinath, S. Karpathy, R. Sharmaand D. Stone. West Indian Veterinary Journal (Communicated).
  12. Differential gene expression analysis of in vitro duck hepatitis B virus infected primary duck hepatocyte cultures. Nair S, Arathy DS, Issac A, Sreekumar E.Virol J. 2011 Jul 23;8:363.
  13. Functional characterization of the CC chemokine RANTES from Pekin duck (Anas platyrhynchos)(2010).D.S. Arathy, Sajith Nair, SojaSagharSoman, AneeshIssac&E.Sreekumar. Dev Comp Immunol. 2011 , 35(1):142-50.
  14. Virological and Serological Surveillance of Avian Influenza Virus in the Birds of Grenada.ArathySabarinath, Gopalakrishnan P Sabarinath, Keshaw P Tiwari, Sachin M Kumthekar, Derek Thomas, Ravindra N SharmaInternational Journal of Poultry Science 01/2011; 10(8):579-582.
  15. SeroprevalenceOf Infectious Bronchitis Virus In Birds Of Grenada.ArathySabarinath, Gopalakrishnan P. Sabarinath, Keshaw P. Tiwari, Sachin M. Kumthekar, Derek Thomas, Ravindra N. Sharma. International Journal of Poultry Science. 2011, Vol. 10 Issue 4, p266-268. 3p. 1
  16. Genomic organization and promoter characterization of RANTES, MIP-1β like and MCP-like CC chemokines of domestic duck (Anasplatyrhynchos) (2009). D S Arathy and E Sreekumar .International Journal of Poultry Science 8 (12): 1123-1127, 2009).
  17. Genetic characterization of 2006–2008 isolates of Chikungunya virus from Kerala, South India, by whole genome sequence analysis. (2009) E. Sreekumar, AneeshIssac , Sajith Nair, RamkumarHariharan, M. B. Janki ,D. S. Arathy, R. Regu, Thomas Mathew , M. Anoop ,K. P. Niyas,M. R. Pillai. Virus Genes, Published online on 23 October 2009.
  18. Immunomodulation by duck defensin, Apl_AvBD2: in vitro dendritic cell immunoreceptor (DCIR) mRNA suppression, and B- and T-lymphocyte chemotaxis.Soman SS, Nair S, Issac A, Arathy DS, Niyas KP, Anoop M, Sreekumar E. MolImmunol. 2009 Sep;46(15):3070-5.
  19. Discovery of Anasplatyrhynchos avian beta-defensin 2 (Apl_AvBD2) with antibacterial and chemotactic functions.Soman SS, Arathy DS, Sreekumar E. MolImmunol. 2009 Jun; 46(10):2029-38.
  20. Synthesis and characterization of novel water soluble polyamide based on spermine and aspartic acid as a potential gene delivery vehicle (2008). B M Viola, T E Abraham, D S Arathy, E Sreekumar, M R Pillai, T J Thomas, C K S Pillai. eXPRESS Polymer Letters Vol.2, No.5 (2008) 330-338.
  21. Molecular characterization and expression of Interferon-gamma of Asian elephant (Elephasmaximus).Sreekumar E, Janki M B, Arathy D S, Hariharan R, Premraj C A, Rasool T J. Vet ImmunolImmunopathol. 2007 May 3.
  22. Identification and sequence characterization and analysis of expression profile of three novel CC chemokines from domestic duck (Anasplatyrhynchos) E Sreekumar, AvinashPremraj, D S Arathy and T J Rasool. Immunogenetics (2005) 47:364-373.
  23. Practical procedures in in vitro fertilization. M S Chauhan and Arathy D SCompendium of short course on Ovum pick-up technology for in vitro production of embryos from elite farm animals organized by CAS in Veterinary Physiology, IVRI (2003).
  24. Ultra sound guided ovum pick-up for IVF in buffalos. M S Chauhan and Arathy D SCompendium of summer school on Artificial breeding and Reproduction management in buffaloes organized by CIRB, Hisar (2003).
Abstracts
  1. Clonal Purification and Evaluation of Transposon Mutants of Ehrlichiachaffeensisin Inducing Host Response and Conferring Protection against Wild-type Infection Challenge.Arathy D. S. Nair, Chuanmin Cheng, Deborah Jaworski, and Roman R. Ganta. Oral presentation in the 26th ASR Meeting  held in Portland, ME from June 15-18, 2013.
  2. Comparative experimental infection study in dogs with five tick-borne Anaplasmataceae pathogens; Ehrlichiacanis, E. chaffeensis, E. ewingii, Anaplasmaphagocytophilumand
    A. platys.
    Arathy D. S. Nair, Chuanmin Cheng, Chanran K. Ganta, Katherine A Sayler, Arthur R. Alleman, Ulrike G. Munderloh and Roman R. Ganta. Oral presentation, CRWAD Meeting,December 8-10, 2013.
  3. Chuanmin Cheng, Arathy D.S. Nair,Ulrike G. Munderloh, Deborah Jaworski, and Roman R. Ganta.EhrlichiachaffeensisMutants Persist in White-Tailed Deer and Ticks.Poster presentation in the 25th ASR Meeting held in Park City, UT from July 28-31, 2013.
  4. Arathy DS Nair, Chuamin Cheng, Deborah Jarwoski, A Gupta and Roman R GantaEvaluatio­­n of white-tailed deer as an animal infection model forEhrlichiachaffeensis. Accepted for poster presentation in the 25th ASR Meeting to be held in Park City, UT from July 28-31.
  5. Ravindra N. Sharma, ArathySabarinath, Guillaume Vanpee, Guillaume Belot,Vanessa Matthew, Claude DeAllie. Bacterial Contamination of Commercial Chicken Eggs in Grenada, West Indies.147th AVMA convention held at Atlanta, GA, USA from July31- August3. 2011.
  6. Arathy D.S., AneeshIssac, SojaSghar.S. &E.Sreekumar.Duck cc chemokines exhibit antiviral and antimicrobial activity in vitro. 10thAvian Immunology Research Group Meeting held at Goldcost, Brisbane, Australia from 24/06/2008 to 27/06/2008.
  7. Arathy D.S., AvinashPremraj, AneeshIssac., Anjali Rajan Thomas., SojaSagharSoman and E. Sreekumar (2007). Duck RANTES( Regulated Upon Activation Normal T Cell Expressesd and Secreted ): a CC chemokine with direct antivial activity against enveloped avian viruses Abstract  for poster presentation in The Bangkok International Conference on Avian Influenza: Integration from Knowledge to control on 23-25 January 2008, The DusitThani Bangkok, Thailand. Bagged the Travel Grant award to attend the conference.
  8. SojaSagharSoman., D.S. Arathy, Anjali Rajan Thomas, AneeshIssac and E. Sreekumar (2007). Insights into the innate immune system of domestic duck (Anasplatyrhynchos)- Investigations on the role of duck beta-defensin in immune response to viruses. Abstract accepted for poster presentation in The Bangkok International Conference on Avian Influenza: Integration from Knowledge to control on 23-25 January 2008, The DusitThani Bangkok, Thailand. Bagged the Travel Grant award to attend the conference.
  9. Molecular characterization and tissue expression analysis of duck CC-chemokines.D. S. Arathy, SojaSagharSoman, & E. Sreekumar. International Conference on New Horizons in Biotechnology ,November 26-29, 2007, Trivandrum, India .
  10. Molecular characterization of newly identified duck RANTES CC- chemokine homologue and functional studies using a chicken B-cell line, DT40. D. S. Arathy, SojaSagharSoman& E. Sreekumar National symposium on “veterinary biotechnology and immunology for sustainable rural livelihood security”February 15-17, 2007. College of Veterinary and Animal Sciences, Pookot,Lakkidi P.O., Wayanad, Kerala.
  11. Biological characterization of a duck cc-chemokine, macrophage inflammatory protein-1 beta (mip-1β)D. S. Arathy, SojaSagharSoman& E. Sreekumar 19thKreala science congress 2007. 29-31 January, Kannur, Kerala.
  12. Molecular and functional characterization of Anatin-a novel beta-defensin peptide. SojaSagharSoman, Arathy D. S and Sreekumar E19thKreala science congress 2007. 29-31 January, Kannur, Kerala.
  13. Discovery of a novel β-defensin antimicrobial peptide Anatin-2 with potent antibacterial and immunomodulatory functions.SojaSagharSoman, D S Arathyand E sreekumar. Fourth Indo-Australian Conference on Biotechnology on May 7-9, 2007 in Brisbane, Australia.
  14. Identification and cDNA characterizationof three novel CC chemokines from domestic duck (Anasplatyrhynchos). E Sreekumar, AvinashPremraj, D S Arathy and T J Rasool. The XI Annual convention of ISVIB and National Symposium on Alternate Strategies for Livestock Production: Role of Biotechnology and Immunology, Nov 3-4, 2004.
  15. Characterization of AB 330: A novel CC chemokine from domestic duck (Anasplatyrhynchos). D S Arathy, E Sreekumar and T J Rasool. The XI Annual convention of ISVIB and National Symposium on Alternate Strategies for Livestock Production: Role of Biotechnology and Immunology, Nov 3-4, 2004.
  16. Effect of specific gravity of culture medium on in vitro embryo development in buffalo. D S Arathy, AshisSaha, GT Sharma, AC Majumdar and MS Chauhan. Proceedings of Annual Conference of International Embryo Transfer Society (IETS) Reproduction fertility and development p-253 , (2004).
  17. Effect of HEPES buffered media on in vitro maturation and fertilization of buffalo oocytes. AshisSahaD S Arathy , GT Sharma, AC Majumdar and MS Chauhan Compendium of XIX National Symposium and Annual Convention of Indian Society of Study on Animal Reproduction (2003).
  18. Effect of duration of oocyte culture on in vitro fertilization in buffalo. D S Arathy, AshisSaha, GT Sharma, AC Majumdar and MS Chauhan Compendium of XIX National Symposium and Annual Convention of Indian Society of Study on Animal Reproduction (2003).
  19. In vitro development of the buffalo embryos in simple medium supplemented with gelatin. D S Arathy, AshisSaha, GT Sharma, AC Majumdar and MS Chauhan. Compendium of Annual Symposium of International Society of Veterinary Immunology and Biotechnology (2003)

 

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