hong-lin-chan
Hong-Lin Chan, PhD
Professor of Medical Science
  • Institute of Bioinformatics and Structural Biology
    National Tsing Hua University
    Hsinchu City, Taiwan
    Phone: 03-5742476
    E-mail: hlchan@life.nthu.edu.tw

Education

2001-2005 PhD in Biochemisrty and Molecular Biology University College, University of London, UK
1994-1996 MSc in Life Sciences National Tsing-Hua University, Taiwan

Biography

Dr Hong-Lin Chan is head of the National Tsing-Hua University (Taiwan) for Quantitative Proteomics Group and has 10 years of experience in proteomic method development and application. He received his PhD degree from University College, University of London in 2005. After 2 year post-doctoral training in the wolfson institute for Biomedical Sciences, he took the current Professorship from National Tsing-Hua University in Taiwan. His group has expertise in the preparation, separation and quantification of proteins and post-translational modifications using mass spectrometry and other methods. Dr Chan was one of the first users of 2D-DIGE technology which is routinely used for protein expression profiling and the group has also established platforms which perform quantitative phosphoproteomics and redox-proteomics analysis. His group is focused on: Serum biomarker discovery, characterising redox and UV stress responses in cell models, mechanisms of cellular signalling, proteomics based studies on breast cancer, prostate cancer and drug resistance formation. Methods include cell biology facilities, 2D-DIGE/MS and quantitative 2D-LC-MS/MS expression profiling.

Research Interests

Cancer signaling, Mass spectrometry, Organelle proteomics, Tumor marker discovery, Phamacoproteomics, Translational medicine (Serum proteomics; body fluid proteomics), Microbiota analysis, Stem cell proteomics, Serum biomarker discovery, Characterising redox stress responses in cell models, Mechanisms of cellular signalling, and Proteomics-based studies on breast cancer, Prostate cancer, and Drug resistance formation.


Scientific Activities

Teaching Experience
2014-Present Professor of Biological Information and Structural Biology, National Tsing Hua University, Taiwan
2011-2014 Associate Professor of Biological Information and Structural Biology, National Tsing Hua University, Taiwan
2007-2011 Assistant Professor, Institute Bioinformatics and Structural Biology, National Tsing Hua University, Taiwan
Honors & Awards
  • Overseas Research Scholarship (ORS)
  • UCL Graduate School Research Scholarship
  • Directed graduate student Chen Yijun received Shen Dachen Foundation Outstanding Research Paper Award
  • Zhang Ruofan, a student in the Department of Biosciences, won the 102-year National Science Foundation subsidized college student research project
  • Guide graduate student Huang Huiru to participate in cross-strait Tsinghua Graduate Academic Forum Outstanding Paper Award
  • Directed graduate student Luo Jingyi won the Shen Dafan Foundation Outstanding Research Paper Award
  • Mentor Chen Jingyi received Shen Dafang Foundation Outstanding Research Paper Award
  • Mentor graduate student Li Min won Shen Dustin Foundation Outstanding Research Paper Award
  • Zhang Ruofan, a university student guiding the university, received the Distinguished Research Paper Award of Shen Dafu Foundation
  • Department of Health Department of Outstanding Department of Friends Award
  • Guidance graduate student Lai Zi Jia won the Sixth Academy of Life Sciences poster contest win
  • Tsinghua University teachers 99 years of academic excellence award
  • National Tsing Hua University Academy of ASTRI new teachers research award
  • Guiding graduate student Lai Zijia and Chen Yijie respectively won masterpieces of the Seventh Academy of Syllabus
  • Lu Yingjie, a master graduate student, won the Eighth Academy of Science Academy Poster Contest
  • National Tsing Hua University Academy of ASTRI new teachers research award
  • Outstanding academic research publishing award
  • Instruct doctoral student graduate student Linsing Ting won the Best Poster Contest of Academy of Science in 2012
  • 101 annual academic excellence award case
  • National Tsing Hua University Research Award for new personnel
  • Outstanding academic research publishing award
  • National Tsing Hua University School of Life Sciences Outstanding Teaching Award
  • 102 year academic excellence award case
  • Instruct doctoral student graduate student Linsing Ting won the 2013 Essay Contest in Biomedical Engineering Institute
  • Chen Yijun, a doctoral candidate mentor, won the 2013 Chinese Academy of Systematics winning contest
  • Chen Jingyi, a master graduate student mentor, won the Best Poster Contest 2013 in Chinese Academy of Science
  • Instruct doctoral student graduate student Linsing Ting won the Best Poster Contest 2013 in the Academy of Science
  • Chen Jingyi, a master graduate student, won the Best Poster Contest 2014 Biomedical Engineering Institute
  • Master Chen Guoguang graduate master's program won the 2014 Academy thesis contest
  • Master Instructor Luo Jingtao won the Best Poster Contest 2013
  • Zhang Ruofan, director of the Department of Biology at the Department of Biosciences, received a special prize in the 2014 Poster Contest of CASS
  • National Tsinghua University to attract and retain special talents of flexible salary and reward subsidies

Publications

  1. Jhen-Wei Ruan, Sarah Statt, Chih-Ting Huang, Yi-Ting Tsai, Cheng-Chin Kuo, Hong-Lin Chan, Yu-Chieh Liao, Tse-Hua Tan, and Cheng-Yuan KAO (2016) Dual-specificity phosphatase 6 deficiency regulates gut microbiome and transcriptome response against diet-induced obesity in mice. Nature Microbiology. In press.
  2. Ching-Hsiang Fan, Chien-Yu Ting, Chung‐Yin Lin, Hong-Lin Chan, Yuan-Chih Chang, You-Yin Chen, Hao-Li Liu, Chih-Kuang Yeh (2016) Noninvasive, Targeted, and Non-viral Ultrasound-Mediated Gene Delivery for Parkinson’s Disease Treatment. Scientific Reports. In press.
  3. Hsiu-Chuan Chou, Jing-Yi Chen, Dai-Ying Lin, Yueh-Feng Wen, Chi-Chen Lin, Sheng-Hao Lin, Ching-Hsiung Lin, Ting-Wen Chung, En-Chi Liao, Ying-Jen Chen, Yu-Shan Wei, Yi-Ting Tsai, and Hong-Lin Chan* (2016) Identification of up- and down-regulated proteins in pemetrexed-resistant human lung adenocarcinoma: flavin reductase and calreticulin play key roles in the development of pemetrexed-associated resistance. J. Proteome Research. In press.
  4. Chi-Chen Lin, Jing-Ting Chen, Meng-Wei Lin, Chia-Hao Chan, Yueh-Feng Wen, Shin-Bei Wu, Ting-Wen Chung, Kevin W. Lyu, Hsiu-Chuan Chou and Hong-Lin Chan* (2015) Identification of protein expression alterations in gefitinib-resistant human lung adenocarcinoma: PCNT and mPR play key roles in the development of gefitinib-associated resistance. Toxicology and Applied Pharmacology. In press.
  5. Ming-Kun Liu, Jie-Zue Lin, Tzyy-Rong Jinn, Hong-Lin Chan*, tzong-yuan Wu * (2015) Identification of Rhopalosiphum padi virus 5' untranslated region sequences required for cryptic promoter activity and internal ribosome entry. International Journal of Molecular Sciences. In press.
  6. Yi-Wen Lo, Szu-Ting Lin, Shing-Jyh Chang, Chia-Hao Chan, Kevin W. Lyu, Jo-Fan Chang, Eugenie Wong Soon May, Dai-Ying Lin, Hsiu-Chuan Chou and Hong-Lin Chan* (2015) Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer. Journal of Cellular and Molecular Medicine. In press.
  7. Szu-Ting Lin, Eugenie Wong Soon May, Jo-Fan Chang, Ren-Yu Hu, Hong-Lin Chan* (2015) PGRMC1 Contributes to Doxorubicin-Induced Chemoresistance in Uterine Sarcoma. Cellular and Molecular Life Science. In press.
  8. Yu-Kuo Chen, Hsin-Chieh Wang, Chi-Tang Ho, Shiming Li, Hong-Lin Chan, Ting-Wen Chung, Kok-Tong Tan, Yi-Ron Li and Chi-Chen Lin. (2015) 5-Demethylnobiletin promotes the formation of polymerized tubulin, Leads to G2/M phase Arrest, and induces autophagy via JNK activation in human lung cancer. Journal of Nutritional Biochemistry. In press.
  9. Chung TW, Tan KT, Chan HL, Lai MD, Yen MC, Li YR, Lin SH, Lin CC. (2014) Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic Activity Contributes to Interferon-Gamma Induced Apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells. Asian Pacific Journal of Cancer Prevention. In press.
  10. Hui-Ju Huang, Chi-Chen Lin, Hsiu-Chuan Chou, Yi-Wen Chen, Szu-Ting Lin, Yi-Chieh Lin, Dai-Ying Lin, Kevin W. Lyu and Hong-Lin Chan* (2014) Proteomic analysis of rhein-induced cytotoxicity: ER stress mediates the cell death in breast cancer cells. Molecular Biosystems. In press.
  11. Eugenie Wong Soon May, Szu-Ting Lin, Chi-Chen Lin, Jo-Fan Chang, Eric Hung, Yi-Wen Lo, Li-Hsun Lin, Ren-Yu Hu, Chi-Lun Feng, Dai-Ying Lin, Shine-Bei Wu, Wen-Chi Lee, Kevin W. Lyu, Hsiu-Chuan Chou and Hong-Lin Chan* (2014) Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: Reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance. Pharmacological Research. In press.
  12. Jing-Jing Chuang, Yuan-Chang Dai, Yung-Lun Lin, Yang-Yi Chen, Wei-Han Lin, Hong-Lin Chan, Yi-Wen Liu Downregulation of glutathione S-transferase M1 protein in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced mouse bladder carcinogenesis. Toxicology and Applied Pharmacology. In press.
  13. Fan Tu, Jiahn-Haur Liao, Feng-Ling Yang, Nien-Tsung Lin, Hong-Lin Chan* and Shih-Hsiung Wu (2014) Lon protease affects the RdxA nitroreductase activity and metronidazole susceptibility in Helicobacter pylori. Helicobacter. In press.
  14. Jo-Fan Chang, Szu-Ting Lin, Eric Hung, Yi-Ling Lu, Eugenie Wong Soon May, Yi-Wen Lo, Hsiu-Chuan Chou and Hong-Lin Chan* (2014) Nuclear proteomics with XRCC3 knockdown to reveal the development of doxorubicin-resistant uterine cancer. Toxicological Science. 139(2), 396-406.
  15. Li-Hsun Lin, Shing-Jyh Chang, Ren-Yu Hu, Meng-Wei Lin, Szu-Ting Lin, Shun-Hong Huang, Ping-Chiang Lyu, Hsiu-Chuan Chou, Zih-Yin Lai, Yung-Jen Chuang and Hong-Lin Chan* (2014) Biomarker Discovery for Neuroendocrine Cervical Cancer. Electrophoresis. 35, 2039-2045.
  16. Chen W. T. Wu Y. L. Chen T. Chang C. S. Chan H.L. Chou H. C. Chen C. Y. Yin H. S. (2014) Proteomics Analysis of the DF-1 Chicken Fibroblasts Infected with Avian Reovirus Strain S1133. PLoS ONE. 9(3), e92154.
  17. Hsiu-Chuan Chou and Hong-Lin Chan* (2014) 5-methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury. Archives of Biochemistry and Biophysics. 543, 15-22.
  18. Hsiu-Chuan Chou and Hong-Lin Chan* (2014) Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma. Proteome Science. 12(1), 2.
  19. Chiao-Yuan Fan, Hsiu-Chuan Chou, Yi-Wen Lo, Yueh-Feng Wen, Yi-Chih Tsai, Haimei Huang and Hong-Lin Chan* (2013) Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells. Electrophoresis. 34, 3305-3314.
  20. Jing-Yi Chen, Hsiu-Chuan Chou, You-Hsuan Chen and Hong-Lin Chan* (2013) High Glucose-Induced Proteome Alterations in Hepatocytes and Its Possible Relevance to Diabetic Liver Disease. Journal of Nutritional Biochemistry. 24, 1889-1910.
  21. Ji-Min Li, Hsiu-Chuan Chou, Ssu-Han Wang, Chieh-Lin Wu, Yi-Wen Chen, Szu-Ting Lin, Yu-Hua Chen and Hong-Lin Chan* (2013) Hyaluronic acid-dependent protection against UVB-damaged human corneal cells. Environmental and Molecular Mutagenesis. 54, 429-449.
  22. Szu-Ting Lin, Yi-Wen Lo, Shing-Jyh Chang, Wen-Ching Wang, Margaret Dah-Tsyr Chang, Ping-Chiang Lyu, Yi-Wen Chen, Hsiu-Chuan Chou and Hong-Lin Chan*(2013) Redox-proteomic analysis of doxorubicin resistance-induced altered thiol activity in uterine carcinoma. Journal of Pharmaceutical and Biomedical Analysis. 78-79, 1-8.
  23. Yi-Wen Chen, Hsiu-Chuan Chou, Szu-Ting Lin, You-Hsuan Chen, and Hong-Lin Chan* (2013) Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury. Evidence-Based Complementary and Alternative Medicine. 2013, 364519.
  24. Szu-Ting Lin, Hsiu-Chuan Chou, Yi-Wen Chen and Hong-Lin Chan*. (2013) Redox-proteomic analysis of doxorubicin-induced altered thiol activity in cardiomyocytes. Molecular Biosystems. 9, 447-456.
  25. Tian-Ren Lee, Hsiao-Yun Lee1, Shun-Hong Huang, Hsin-Tzu Chan, Hong-Lin Chan* and Ping-Chiang Lyu. (2013) Comparative Proteomic Analysis of Normal and Memory Deficient Drosophila melanogaster Head. Zoological studies. 52, 10.
  26. Chieh-Lin Wu, Hsiu-Chuan Chou, Ji-Min Li, Yi-Wen Chen, Jui-Hsiang Chen, Yu-Hua Chen and Hong-Lin Chan* (2013) Hyaluronic acid-dependent protection against alkali-burned human corneal cells. Electrophoresis. 33, 1-9.
  27. Chun-Liang Tung, Szu-Ting Lin, Hsiu-Chuan Chou, Yi-Wen Chen, Hwan-Chung Lin, Chung-Liang Tung, Kao-Jean Huang, Yi-Ju Chen, Ying-Ray Lee, and Hong-Lin Chan* (2013) Proteomics-based identification of plasma biomarkers in oral squamous cell carcinoma. Journal of Pharmaceutical and Biomedical Analysis. 75, 7-17.
  28. Chieh-Hsiang Lu, Szu-Ting Lin, Hsiu-Chuan Chou, Ying-Ray Lee, and Hong-Lin Chan* (2013) Proteomic Analysis of Retinopathy-related Plasma Biomarkers in Diabetic Patients. Archives of Biochemistry and Biophysics. 529, 146-156.
  29. Ching-Hsuan Law, Ji-Min Li, Hsiu-Chuan Chou, Yu-Hua Chen, and Hong-Lin Chan* (2013) Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia reperfusion injury and treatment. Toxicology. 303, 54-71.
  30. Chih-Ping Chen, Tzu-Chia Lai, Schu-Rern Chern, Sheng-Hsiang Li, Hsiu-Chuan Chou, Yi-Wen Chen, Szu-Ting Lin, Ying-Chieh Lu, Chieh-Lin Wu and Hong-Lin Chan* (2013) Proteome Differences between Male and Female Fetal Cells in Amniotic Fluid.OMICS: A Journal of Integrative Biology. 17, 16-26.
  31. Ying-Ray Lee, Yi-Wen Chen, Ming-Chih Tsai, Hsiu-Chuan Chou, and Hong-Lin Chan* (2012) Redox- and Expression- Proteomic Analysis of Plasma Biomarkers in Bladder Transitional Cell Carcinoma.Molecular Biosystems. 8, 3314-3324.
  32. You-Hsuan Chen, Jing-Yi Chen, Yi-Wen Chen, Szu-Ting Lin and Hong-Lin Chan* (2012) High Glucose-Induced Proteome Alterations in Retinal Pigmented Epithelium Cells and Its Possible Relevance to Diabetic Retinopathy. Molecular Biosystems. 8, 3107-3124.
  33. Hsiu-Chuan Chouand Hong-Lin Chan* (2012) Targeting proteomics to investigate metastasis-associated mitochondrial proteins. Journal of Bioenergetics and Biomembranes. 44(6), 629-634.
  34. Szu-Ting Lin, Hsiu-Chuan Chou, Shing-Jyh Chang, Yi-Wen Chen, Ping-Chiang Lyu, Wen-Ching Wang, Margaret Dah-Tsyr Chang and Hong-Lin Chan* (2012) Proteomic Analysis of Proteins Responsible for the Development of Doxorubicin Resistance in Human Uterine Cancer Cells. Journal of Proteomics. 75 (18) 5822-5846.
  35. You-Hsuan Chen, Hsiu-Chuan Chou, Szu-Ting Lin, Yi-Wen Chen, Yi-Wen Lo and Hong-Lin Chan* (2012) Effect of High Glucose on Secreted Proteome in Cultured Retinal Pigmented Epithelium Cells: Its Possible Relevance to Clinical Diabetic Retinopathy. Journal of Proteomics. 77 (1) 111-128.
  36. Jing-Yi Chen, Hong-Lin Chan, Hsiu-Chuan Chou (2012) Proteomic analysis of quercetin-induced cardioprotective effects. Genomic Medicine, Biomarkers, and Health Sciences. 4, 51-53.
  37. Li-Hsun Lin, Hong-Lin Chan, Hsiu-Chuan Chou (2012) DNA-dependent protein kinase regulated glioblastoma survival in doxorubicin-induced cytotoxicity. Genomic Medicine, Biomarkers, and Health Sciences. 4, 54-56.
  38. Yi-Wen Lo, Hong-Lin Chan, Hsiu-Chuan Chou (2012) Proteomic analysis of mitochondrial proteins in doxorubicin-resistant cancer cells. Genomic Medicine, Biomarkers, and Health Sciences. 4, 57-60.
  39. Chih-Ping Chen, You-Hsuan Chen, Schu-Rern Chern, Shing-Jyh Chang, Te-Lung Tsai, Sheng-Hsiang Li, Hsiu-Chuan Chou, Yi-Wen Lo, Ping-Chiang Lyu and Hong-Lin Chan* (2012) Placenta Proteome Analysis from Down Syndrome Pregnancies for Biomarker Discovery. Molecular Biosystems. 8, 2360-2372.
  40. Chia-Ching Chen, Ying-Chieh Lu, Yi-Wen Chen, Wen-Li Lee, Chieh-Hsiang Lu, You-Hsuan Chen, Yun-Ching Lee, Szu-Ting Lin, John F. Timms, Ying-Ray Lee, Hsiu-Chuan Chou and Hong-Lin Chan* (2012) Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: role of glucose-induced ROS. Journal of Proteomics. 75(12), 3760-3777.
  41. Hsin-Tzu Chan, Tian-Ren Lee, Shun-Hong Huang, Hsiao-Yun Lee, Tzu-kang Sang, Hong-Lin Chan*, Ping-Chiang Lyu (2012) Proteomic Analysis of a Drosophila IBMPFD Model Reveals Potential Pathogenic Mechanisms. Molecular Biosystems. 8(6), 1730-1741.
  42. Tian-Ren Lee, Shun-Hong Huang, Chi-Ching Lee, Hsiao-Yun Lee, Hsin-Tzu Chan, Kuo-Sen Lin, Hong-Lin Chan*, Ping-Chiang Lyu* (2012) Proteome Reference Map of Drosophila melanogaster Head. Proteomics.12(11), 1875-1878.
  43. Hsiu-Chuan Chou, Ying-Chieh Lu, Chao-Sheng Cheng, Yi-Wen Chen, Ping-Chiang Lyu, Cheng-Wen Lin, John F. Timms and Hong-Lin Chan* (2012) Proteomic and redox-proteomic analysis of berberine-induced cytotoxicity in breast cancer cells. Journal of Proteomics. 75(11)3158-3176.
  44. Hong-Lin Chan*, Sinclair J, Timms JF. (2012) Proteomic Analysis of Redox-Dependent Changes Using Cysteine-Labeling 2D DIGE. Methods in Molecular Biology. 854, 113-128.
  45. Chieh-Lin Wu, Hsiu-Chuan Chou, Chao-Sheng Cheng, Ji-Min Li, Szu-Ting Lin, Yi-Wen Chen and Hong-Lin Chan* (2012) Proteomic Analysis of UVB-Induced Protein Expression- and Redox-Dependent Changes in Skin Fibroblasts using Lysine- and Cysteine-Labeling Two-Dimensional Difference Gel Electrophoresis. Journal of Proteomics. 75(7)1991-2014.
  46. Chao-Po Lin, Yi-Wen Chen, Wen-Hsin Liu, Hsiu-Chuan Chou, Yi-Ping Chang, Szu-Ting Lin, Ji-Min Li, Shiou-Fen Jian, Ying-Ray Lee and Hong-Lin Chan* (2012) Proteomic identification of plasma biomarkers in uterine leiomyoma. Molecular Biosystems.8(4)1136-1145.
  47. Ching-Yao Chang*, Hong-Lin Chan*, Hui-Yi Lin, Tzong-Der Way, Ming-Ching Kao, Ming-Zhang Song, Ying-Ju Lin, Cheng-Wen Lin. (2012) Rhein induces apoptosis of HER2-overexpressing breast cancer cells. Evidence-Based Complementary and Alternative Medicine. 2012, 952504.
  48. Yi-Wen Chen, Jieh-Yuan Liu, Szu-Ting Lin, Ji-Min Li, Shun-Hong Huang, Jing-Yi Chen, Jing-Yiing Wu, Cheng-Chin Kuo, Chieh-Lin Wu, Ying-Chieh Lu, You-Hsuan Chen, Chiao-Yuan Fan, Ping-Chun Huang, Ching-Hsuan Law, Ping-Chiang Lyu, Hsiu-Chuan Chou and Hong-Lin Chan* (2011) Proteomics analysis of Gemcitabine-induced drug resistance in pancreatic cancer cells. Molecular Biosystems. 7, 3065-3074.
  49. Peir-Haur Hung, Yi-Wen Chen, Kuang-Chi Cheng, Hsiu-Chuan Chou, Ping-Chiang Lyu, Ying-Chieh Lu, Ying-Ray Lee, Cheng-Tao Wu and Hong-Lin Chan* (2011) Plasma Proteomic Analysis the Role of Critical Limb Ischemia in Diabetic Patients with Hemodialysis. Molecular Biosystems. 7, 1990-1998.
  50. Yi-Wen Chen, Hsiu-Chuan Chou, Ping-Chiang Lyu, Hsien-Sheng Yin, Fang-Liang Huang, Wun-Shaing Wayne Chang, Chiao-Yuan Fan, I-Fan Tu, Tzu-Chia Lai, Szu-Ting Lin, Ying-Chieh Lu, Chieh-Lin Wu, Shun-Hong Huang and Hong-Lin Chan (2011) Mitochondrial proteomics analysis of tumorigenic and metastatic breast cancer markers. Functional and Integrative Genomics. 11, 225-239.
  51. Peir-Haur Hung, Ying-Chieh Lu,Yi-Wen Chen, Hsiu-Chuan Chou, Ping-Chiang Lyu, Ying-Ray Lee and Hong-Lin Chan (2010) Proteomic identification of plasma signatures in type 2 diabetic nephropathy. Journal of Integrated OMICS.1, 151-156.
  52. Jenny Worthington, Mariana Bertani, Hong-Lin Chan, Bertran Gerrits and John F Timms (2010) Transcriptional profiling of ErbB signalling in mammary luminal epithelial cells-interplay of ErbB and IGF1 signalling through IGFBP3 regulation. BMC Cancer 10, 490.
  53. Hsiang-Ling Huang, Hsiang-Wei Hsing,Tzu-Chia Lai, Yi-Wen Chen, Tian-Ren Lee, Hsin-Tsu Chan, Ping-Chiang Lyu, Chieh-Lin Wu, Ying-Chieh Lu, Szu-Ting Lin, Cheng-Wen Lin, Chih-Ho Lai, Hao-Teng Chang, Hsiu-Chuan Chou and Hong-Lin Chan* (2010) Trypsin-induced Proteome Alteration during Cell Subculture in Mammalian Cells. Journal of Biomedical Science. 17(1), 36.
  54. Hsiu-Chuan Chou, Yi-Wen Chen, Tian-Ren Lee, Hsin-Tsu Chan, Hsin-Hsin Shen, Ping-Chiang Lyu, John F. Timms and Hong-Lin Chan* (2010) Proteomics Study of the Roles of ROS and Src Kinase Inhibitor on H9C2 Cell: a model cell for Heart Ischemia Reperfusion Injury Study. Free Radical Biology and Medicine. 49, 96-108.
  55. Chih-Ho Lai, Nai-Wen Chang, Chin-Fen Lin, Chia-Der Lin, Ying-Ju Lin, Lei Wan, Jim Jinn-Chyuan Sheu, Shih-Yin Chen, Yi-Ping Huang, Yu-Ting Sing, Tse-Wen Tao, Cheng-Kuo Lai, Ming-Hsui Tsai, Hong-Lin Chan, Yu-Jen Jou, Cheng-Wen Lin (2010) Proteomics-based identification of haptoglobin as a novel plasma biomarker in oral squamous cell carcinoma. Clinica Chimica Acta 411, 984-991.
  56. Chan, H.L., Timms, J.F. (2010) Proteomic analysis of redox-dependent changes using cysteine-labeling two-dimensional difference gel electrophoresis. Methods in Molecular Biology in press.
  57. Tzu-Chia Lai, Hsiu-Chuan Chou, Yi-Wen Chen, Tian-Ren Lee, Hsin-Tsu Chan, Hsin-Hsin Shen, Wei-Ta Lee and Hong-Lin Chan* (2010) Secretomic and Proteomic Analysis of Potential Breast Cancer Markers by Two-Dimensional Differential Gel Electrophoresis. Journal of Proteome Research 9, 1302-1322.
  58. Chan, H.L., Chou, H.C., Duran, M.C.,Gruenewald, J., Waterfield, M.D., Ridley, A. and Timms, J.F. (2010) Major role of EGFR and SRC kinases in promoting oxidative stressdependentloss of adhesion and apoptosis in epithelial cells. Journal of Biological Chemistry 285, 4307-4318.
  59. George, R., Chan, H.L., Ahmed, Z., Suen, K.M., Stevens, C.N., Levitt, J.A., Suhling, K., Timms, J.F. and Ladbury, J.E. (2009) A complex of Shc and Ran-GTPase localises to the cell nucleus. Cellular & Molecular Life Sciences 66, 711-720.
  60. Duran, M.C.,Chan, H.L., Timms, J.F. (2008) Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry. Methods in Molecular Biology 527, 33-45.
  61. Chan, H.L., Gaffney, P., Waterfield, M.D., Anderle, H., Matthiessen, H.P., Schwarz, H.P., Turecek, P.L., Timms, J.F. (2006) Proteomic analysis of UVC irradiation-induced damage of plasma proteins: Serum amyloid P component as a major target of photolysis. FEBS Letters 580, 3229-3236.
  62. Chan, H.L., Gharbi, S, Cramer, R., Gaffney, P., Waterfield, M.D., Timms, J.F. (2005) Proteomics analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine-and lysine-labelling 2D-DIGE. Proteomics 5, 2908-2926.
  63. White, S.L., Gharbi, S., Bertani, M.F., Chan, H.L., Waterfield, M.D., Timms, J.F. (2004) Cellular response to ErbB-2 overexpression in human mammary luminal epithelial cells: comparison of mRNA and protein expression. Br. J. Cancer 90, 173-181.

     
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