stephen-mathew
Stephen O. Mathew
Assistant Professor
  • : 817-735-5407   

Department of Cell Biology & Immunology
UNT Health Scienc Center
USA

Education

1996 – 2000 Ph. D. in Microbiology, Rani Durgavati University, Jabalpur, M.P., India
1993–1995 M.S. in Microbiology, RaniDurgavati University, Jabalpur, M.P., India
1990 –1993 B. S. in Biology, GovernmentScienceCollege, R.D. University, Jabalpur, M.P., India

 

Biography

After completing my MS and Ph.D. in Microbiology from Rani Durgavati University, Jabalpur, India, I worked for a short while in the Pharmaceutical Industry in the area of Clinical research managing several clinical trials. My strong desire towards bench research brought me to UNT Health Science Center at Fort Worth, TX, USA to do Post-Doctoral research in Cancer Immunology in 2002. In 2003, I was promoted to Research Assistant Professor and subsequently in 2013 was appointed as Assistant Professor in the Department of Cell Biology & Immunology, UNT Health Science Center, Fort Worth, TX. My main research focus has been onthe role of Natural Killer (NK) cell receptors in diseases like cancer, HIV and lupus.NK cells are cells of the immune system that form the first line of defense against cancer and infectious diseases.

 

Research Interest

Our research in the past several years has been focused towards unraveling the molecular basis of tumor cell recognition by NK cells and its multiple receptor-ligand interactions. We have also expanded our research in the areas of childhood leukemia, lupus and HIV immunology. Specifically, we have collaborated with rheumatologists to study the role of NK receptors in systemic lupus erythematosus (SLE) and pediatric oncologists, epidemiologists and basic science researchers, to investigate the role of NK cell receptors in acute lymphoblastic leukemia (ALL) in children. The overall goal of our research is to understand the basic mechanisms of these receptors in different disease models so that their function could be modulated for therapeutic interventions to improve patient outcomes.

 

Professional Activities:

Profesional Experience

Assistant Professor of Cell Biology & Immunology Oct. 2013 – present
University of North Texas Health Science Center (UNTHSC), Fort Worth, TX, 76107, USA

Research Assistant Professor of Molecular Biology & Immunology  __May 2003 – Sep. 2013 University of North Texas Health Science Center (UNTHSC), Fort Worth, TX, 76107, USA

  • 11+ years of basic science and clinical research experience in the area of Cancer, Lupus and HIV Immunology.
  • Experienced Principal Investigator and Lab Manager for Cancer Immunology Lab. 
  • Bench research:
    • Determined the functional role of human NK cell receptor 2B4 isoforms.
    • Demonstrated the inhibitory role of 2B4+ CD8+ T cells against HIV epitopes presumably promoting the viral escape mechanisms and AIDS progression.
    • Identified the overexpression of immune receptor LLT1 in prostate cancer cells that enables it to escape from the immune attack.
  • Clinical research:
    • Initiated and conducted clinical translational research in childhood leukemia with pediatric oncologists and epidemiologists at Cook Childrens Medical Center, Fort Worth, TX and UNTHSC.
    • Successfully completed and published a clinical study identifying the role of immune receptor CS1 that has therapeutic implications in systemic lupus erythematosus (SLE) with rheumatologists at John Peter Smith (JPS) Hospital, Fort Worth, TX and UNTHSC.
  • Lecturer in medical and graduate curricula. Topics taught include Microbiology, Clinical Microbiology, Cancer immunology and Molecular Biology. Developed medical cases for Team Based Learning (TBL) initiatives for Medical Sciences and graduate students.
  • Recipient of consistently high teaching evaluations.
  • Developed and directed Microbiology and Immunology core course for MS in Medical Sciences (Course Director and coordinated 4 faculty and 160 students/yr).
  • Spearheaded collaborative teams with Pediatric Oncologists, Epidemiologists, Rheumatologists, Pharmacology & Neuroscience experts and basic science researchers for Clinical and translational research.
  • Mentored 2-MS student, co-mentored 2-MS and 5-Ph.D students and trained 27 undergraduate and graduate students. Currently, co-mentoring 1-MS student.
  • Mentored a junior faculty from Savanah State University, Savanah, GA on grant writing under the STAR Fellowship program of Texas center for Health Disparities (TCHD).
  • Served on Institutional committees: Institutional Animal Care and Use Committee (IACUC), Research advisory committees for Ph.D. and MS students, Oral Qualifying and Grant Writing Exam committees, Medical School Admissions Interview panel, Health Promotion committee and Wellness taskforce.

Post Doctoral Research Associate, Cancer Immunology__June 2002 – May 2003 University of North Texas Health Science Center, Fort Worth, Texas, USA.

  • Conducted receptor-ligand interactions of human 2B4 and CD48 in human NK cells.
  • Identified amino acid Lysine and Glutamic acid to play a critical role in the interaction of natural killer (NK) cell receptor 2B4 with its ligand CD48 in the functional activation of NK cells.

Manager, Clinical Research__Apr. 2002 – May 2002 Medical Affairs, Emcure Pharmaceuticals Ltd., Pune, India

  • Directed the clinical research division by managing a team of 4 CRAs and reporting to the Vice President of Medical Affairs.
  • Served as a Medical Science Liaison between the Sales and Marketing division and network of physicians.
  • Developed and maintained relationships with key opinion leaders (KOL) and regional healthcare professionals in south west India.

Associate Manager, Clinical Research___Feb. 2001 – Mar. 2002Medical Affairs, Emcure Pharmaceuticals Ltd., Pune, India

  • Initiated, monitored, audited and managed clinical research activities of 80 multicentric clinical trials (Phase III and Phase IV) in compliance with ICH-GCP guidelines.
  • Consistently identified potential investigators, evaluated new sites for clinical investigation and served as advocate for clinical trials at IRB meetings.
  • Regularly delivered presentations at conferences, grand rounds, CME meetings and product launches.
  • Delivered training presentations to new CRAs and medico-scientific support to the sales representatives.
  • Developed Protocols and Case Report Forms (Phase III and IV studies), packaged trial material (blinding, randomization and coding), analyzed data, communicated manuscripts and reports.
  • Developed IND application dossiers for submission to the Drug Controller General of India (DCGI).
  • Regularly collected and interpreted medical insights/intelligence and communicated relevant information to company stakeholders according to standing policies.
  • Provided medical and scientific support for biotechnology projects.

Professional Affiliations

  1. Member of American Association of Immunologists (AAI), 2008 - present.
  2. Member of American Society of Microbiology (ASM), 2004 - present.
  3. Life member of Association of Microbiologists of India (AMI), 1999 - present.
  4. Member, Advocacy Board for Fort Worth Relay for Life, American Cancer Society Cancer Action Network (ACSCAN), 2009 - 2011.
  5. Member of Institute for Cancer Research, UNT Health Science Center, Fort Worth, TX, 2003 – present.
  6. Member, Advisory Board, Texas Best Care Home Health Inc., Mesquite, TX, 2006 - present.

 

Publications

Full length papers – published and in review

  1. Kim, JR, Horton, NC, Mathew, SO and Mathew, PA. 2013. CS1 (SLAMF7) Inhibits Production of Proinflammatory Cytokines by Activated Monocytes. Inflammation Research62 (8): 765-72. PMID: 23695528.
  2. Horton, NC, Mathew, SO and Mathew, PA. 2013. Novel Interaction between Proliferating Cell Nuclear Antigen and HLA I on the Surface of Tumor Cells Inhibits NK Cell Function through NKp44. pLOSOne; 8(3):e59552. doi: 10.1371/journal.pone.0059552. Epub 2013 Mar 19. PMID: 23527218.
  3. Dongre, P., Mathew, SO, Akopova, I, Gryczynski, I and Mathew, PA. 2013. YY1 and a unique DNA repeat element regulates the transcription of mouse CS1 (CD319, SLAMF7) gene.Molecular Immunology54 (3-4): 254-263.PMID: 23318224.
  4. Aldy, KN, Horton, N, Mathew, PA and Mathew, SO. 2011. 2B4+ CD8+ T Cells play an inhibitory role against constrained HIV Epitopes. Biochemical and Biophysical Research Communication405 (3): 503 - 507.PMID: 21256826.
  5. Kim, JR,Mathew SO,Pertusi, R, Patel, RK and Mathew, PA. 2010. Altered expression of SLAMfamily receptors CS1 (CD319) and 2B4 (CD244) in patients with systemic lupus erythematosus. Clinical and Experimental Immunology 160: 348-358. PMID: 20345977.
  6. Bambard, ND, Mathew, SO and Mathew, PA. 2010. LLT1-mediated activation of interferon gamma production in Natural Killer Cells involves ERK signaling pathway. Scandinavian Journal of Immunology 71: 210 – 219.PMID: 20415786.
  7. Mathew SO, Rao, KR, Kim, JR, Bambard, ND and Mathew, PA. 2009. Functional role of human NK cell receptor 2B4 (CD244) isoforms. European Journal of Immunology39 (6): 1632-1641.PMID: 19499526.
  8. Mathew, SO, Rao, KK, Bambard, ND and Mathew, PA. 2008. “Functional Role of Human Natural Killer Cell Receptor 2B4 (CD244) Isoforms”. The FASEB Journal. 2008; 22:1064.14.
  9. Lee, JK, Mathew, SO, Vaidya, SV, Kumaresan, PR and Mathew, PA. 2007. CS1 (CRACC, CD319) induces proliferation and autocrine cytokine expression on human B-lymphocytes. Journal of Immunology 179(7):4672-8.PMID: 17878365.
  10. Mathew, SO, Vaidya, SV, Kim, JR and Mathew, PA. 2007. Human natural killer cell receptor 2B4 (CD244) down-regulates its own expression by reduced promoter activity at an Ets element. Biochemical and Biophysical Research Communication 355(2): 483-7.PMID: 17300754.
  11. Ross, J, Mathew, P, Mathew, S and Bambard N. 2006. Isolation and expression of recombinant fusion protein of Lectin like transcript1 (LLT1). Journal of Mississippi Academy of Sciences51 (1):39.
  12. Mathew, SO, Kumaresan PR, Lee JK, Huynh VT, and Mathew PA. 2005. Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells. Journal of Immunology 175:1005-13.PMID: 16002700.
  13. Amin, RB, Simerpreet, S, Langade, D,Mathew SO and Dewan B. 2001. An open trial to assess Ferric hydroxide Polymaltose Complex plus Folic acid (Orofer) in iron deficiency anemia. Journal of American Medical Association India Physicians Update 4 (10): 53.
  14. Sandhu, SS, Chandrasekharan, U, Rajak, RC and Mathew, SO. 1999. Autolytic enzymes of two entomopathogenic fungi for the isolation of protoplast. Proceedings of National Academy of Sciences., India. Vol. 69 (B), II: 177-181.
  15. Mathew, SO, Sandhu, SS and Rajak, RC. 1998. Bioactivity of Nomuraearileyi against Spilosomaobliqua : Effect of dosage, temperature and relative humidity. Journal of Indian Botanical Society 77: 23 - 25.
  16. Sandhu, SS, Shrivastava, A, Gupta, KD, Verma, S, Grover, S and Mathew SO. 1998. Development of entomopathogenic hybrid IA through biotechnological approaches for the biocontrol of Aedesaegypti.Advances in Medical Entomology and Human Welfare No.1 Supplement, pp. 101-108.

Chapters, reviews, books

  1. Mathew, SO, Nair, M and Sandhu, SS. 2010. Review article - HIV resistance in sex workers – Molecular and Immunological aspects. People’s Post. 1 (46): 49-51.

Abstracts

  1. S. Mathew, J. Kim and P. Mathew. 2014. Characterization of human CS1 gene promoter in NK and B cells. J. Immunology 192 (1 Supplemenkt) 126.10.
  2. S. Mathew, J. Kim and P. Mathew. 2013. CS1 (CRACC, CD319, SLAMF7) inhibits production of proinflammatory cytokines by activated monocytes. 100th American Association of Immunologists Conference Abstracts, pp. 96.
  3. Charisse Tan, W. Paul Bowman, Porunelloor Mathew and Stephen Mathew. 2013. Altered expression of immune receptors 2B4, CS1 and LLT1 in children with Acute Lymphoblastic Leukemia (ALL).Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp. 47.
  4. Mathew, SO, Aldy, KN, Bowman, WP, Fling, J, Zhang, FF and Mathew, PA. 2012. Analysis of expression and function of immune receptors in childhood Acute Lymphoblastic Leukemia (ALL). 7thAnnual Texas Conference on Health Disparities:Battling Breast Cancer Disparities. Pp. 29.
  5. Martin, RC, Horton, N, Mathew, SO and Mathew, PA. 2011. Isolation, purification and function of LLT1 monoclonal antibody. Moorehouse Innovation Expo 2011 MAPP Symposium.Pp. 23.
  6. Mathew, SO, Aldy, KN, Bowman, WP, Fling, J, Zhang, FF and Mathew, PA. 2010. Altered expression and function of immune receptors 2B4 (CD244), CS1 (CD319) and NKp30 (CD337) in childhood Acute Lymphoblastic Leukemia (ALL). CPRIT Innovations in Cancer Prevention Research Conference, Austin, TX. Pp.46.
  7. Mathew, PA, Kim, JR and Mathew, SO. 2010. Inhibitory role of CS1 in cytokine production by LPS-activated monocytes. 33rd World Conference of International Societyof Hematology, Jerusalem, Israel.
  8. Mathew, SO, Kim, JR, Patel, RK, Pertusi, R, and Mathew, PA. 2010. Altered expression of SLAM family receptorCS1 in African American patients with systemic lupus erythematosus (SLE). 14th International Congress of Immunology, Kobe, Japan.pp. i112
  9. Kim, JR, Patel, RK, Mathew, SO, Pertusi, RM and Mathew, PA. 2009. Analysis of SLAM family receptors in SLE. American College of Rheumatology/ARHP Annual Scientific meeting, Philadelphia, PA. P109.
  10. Aldy, KN, Mathew, PA and Mathew, SO. 2009. Identifying HIV epitopes that cause an effective CD8+ T cell response for use in an HIV vaccine. 4th Annual Texas Conference on Health Disparities: Targeting Health Disparities through prevention. pp.39.
  11. Kim, JR, Mathew SO, Pertusi, R, Patel, RK and Mathew, PA. 2009. Altered expression of CS1 in peripheral blood mononuclear cells of African American patients with systemic lupus erythematosus. 4th Annual Texas Conference on Health Disparities: Targeting Health Disparities through prevention. pp. 40.
  12. Kalantarian, N, Mathew, PA and Mathew, SO. 2009. Isolation of Natural killer cell receptor LLT1 recombinant fusion protein. Annual Biomedical Research Conference-Minority Students, Phoenix, AZ.
  13. Mathew, PA and Mathew, SO. 2008. Role of 2B4 (CD244) and CS1 (CD319) in natural killer cell-mediated killing of cancer cells. Abstracts of 8th International Conference of Anticancer Research, Kos, Greece, October 17-22, 2008; 3398-3399.
  14. Rao, K, Bambard, N, Mathew, S and Mathew, P. 2007. Functional role of human 2B4 (CD244) isoforms in natural killer cells.10th Meeting of the Society for Natural Immunity at Cambridge, UK, p46.
  15. Mathew, PA, Kim, JR, Vaidya, SV and Mathew, SO. 2007. Human natural killer cell receptor 2B4 (CD244) down-regulates its own expression by opposing promoter activities at AP-1 and Ets elements.10th Meeting of the Society for Natural Immunity at Cambridge, UK.
  16. Mathew, SO, Kim, JR, Vaidya, SV and Mathew, PA. 2007. Human natural killer cell receptor 2B4 (CD244) down-regulates its own expression by opposing promoter activities at the AP-1 and Ets elements.Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.72.
  17. Kim, JR, Mathew, SO, Pertusi, RM, Patel, RK and Mathew, PA. 2007.  Analysis of expression of immune receptors in subjects with Systemic Lupus Erythematosis.Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.73.
  18. Leake, K, Mathew, SO,Mathew, PA, Kim, JR and Bambard, ND. 2007.CS1 (CD319, CRACC) mediated activation of cytotoxicity in human NK cells involves distinct pathways.Annual Biomedical Research Conference-Minority Students, Austin, TX.
  19. Ross, J, Mathew, P, Mathew, S and Bambard N. 2006. Isolation and expression of recombinant fusion protein of Lectin like transcript1 (LLT1). Journal of Mississippi Academy of Sciences 51 (1):39.
  20. Rao, K, Mathew, S and Mathew, P. 2006. Functional role of human 2B4 isoforms 2B4-A and 2B4-B. Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.29.
  21. Mathew, SO, Kim, JR, Vaidya, SV and Mathew, PA. 2006. Transcriptional regulation of the 2B4 gene in human natural killer cells.Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.68.
  22. Kim, JR, Mathew, SO and Mathew PA. 2006.  Molecular basis for positive and negative signaling by natural killer cell receptor 2B4 (CD244).Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.69.
  23. Mathew, SO, Kumaresan, PR and Mathew, PA. 2005. Mutational and functional analysis of human 2B4 (CD244) - CD48 interaction. Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.54.
  24. Mathew PA, Kumaresan, PR and Mathew, SO. 2004.  The aminoacid residues Lys68 and Glu70 in the variable domain of human 2B4 (CD244) are critical for interaction with CD48. Abstracts of8th Annual meeting of the Society for Natural Immunity and 20th International Natural Killer Cell Workshop April 24-28, 2004 at Noordwijkerhout, The Netherlands, B063.
  25. Mathew, SO, Kumaresan, PR and Mathew, PA. 2004. Structural basis of NK cell activation: Identification of the functional aminoacid residues involved in 2B4-CD48 interaction. Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.48.
  26. Pirooz, AD, Mathew, SO and Mathew, PA. 2004. The molecular characterization of natural cytotoxicity receptor ligands for NKP46 and NKP44. Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.25.
  27. Davies-Balogun, O, Mathew, SO and Mathew, PA. 2003. Mutational Analysis of Novel Tyrosine Motifs in the Cytoplasmic Tail of Human Natural Killer (NK) Cell Receptor, 2B4". Annual Biomedical Research Conference-Minority Students, San Diego, California.
  28. Mathew, SO, Kumaresan, PR, Huynh, VT and Mathew, PA. 2003. The molecular characterization of 2B4 – CD48 interaction by mutational analysis. Abstracts ofResearch Appreciation Day (RAD) of University of North Texas Health Science Center at Fort Worth, TX, pp.61.

Presentation at professional and/or scientific meetings (list only those presentations not listed as a published abstract).

  1. Mathew, SO, Kim, JR, Pertusi, RM, Patel, RK and Mathew, PA. 2009. Analysis of SLAM family receptors 2B4, CS1 and NTB-A in patients with systemic lupus erythematosus (SLE). Poster presentation at 96th Annual Meeting of the American Association of Immunologists-Immunology 2009, Seattle, WA.,pp.29, 49.22.
  2. Mathew, SO, Kim, JR, Pertusi, RM, Patel, RK and Mathew, PA. 2009. Analysis of SLAM family receptors 2B4, CS1 and NTB-A in patients with systemic lupus erythematosus (SLE). Oral presentation at the 3rd National meeting of Association of Scientists of Indian Origin in America (ASIOA), Jan. 2009 at Denton, TX.
  3. Mathew, SO, Bambard, ND and Mathew, PA. 2008. Identification of HIV-gag specific structurally constrained epitopes for use in an HIV vaccine. Poster presented at the 3rd Annual Texas Conference on Health Disparities at Fort Worth, TX. (May 29, 2008).
  4. Zhang, FF, Bowman, WP, Fling, JA, Mathew, PA and Mathew, SO.2008. Molecular characterization of immune receptors and gene-specific hypermethylation in hispanic, caucasian and africanamerican children with acute lymphoblastic leukemia (ALL). Poster presented at the 3rd Annual Texas Conference on Health Disparities at Fort Worth, TX. (May 29, 2008).
  5. Mathew, SO, Rao, KK, Bambard, ND and Mathew, PA. 2008. Functional Role of Human Natural Killer Cell Receptor 2B4 (CD244) Isoforms.Poster presentation at 95th Annual Meeting of the American Association of Immunologists-Immunology and Experimental Biology Conference 2008, San Diego, CA, 22:1064.14.
  6. Mathew, SO, Bambard ND and Mathew, PA. 2007. Identification of MHC-I structurally constrained HIV epitopes for use in an HIV vaccine. Poster presented at the 2nd Annual Texas Conference on Health Disparities at Fort Worth, TX, pp.27 (May 31, 2007)
  7. Mathew, SO, Kim, JR, Vaidya, SV and Mathew, PA. 2007.Human NK cell receptor 2B4 (CD244) down-regulates its own expression. Oral presentation at the 2nd National meeting of Association of Scientists of Indian Origin in America (ASIOA), Jan. 2007 at Denton, TX.

Continuing Education

  1. Blood-borne Pathogens training, UNTHSC, 2014.
  2. Workshop on Applying the Quality Matters rubric for Distance education and online courses, August 2013.
  3. Workshop on Statistical analysis using SPSS, February 2013.
  4. Team based Learning workshop with Nancy Houfek - Powerful Teaching: From Active Presentation to Active Learning, Dec. 2012.
  5. Institutional Animal Care and Use Committee (IACUC) 101 & 201 training at MD Anderson Cancer Center, Houston, TX, 2011.
  6. Workshop on Team Based Learning (TBL) and MedEd Portal at UNT Health Science Center, Fort Worth, TX, March, June and August2011.
  7. Training and certification to qualify as a Phlebotomist, 2009.
  8. Workshop on “developing outcome based assessments”, “writing and validating test items”, “evaluating students, courses and programs” at UNT Health Science Center, Fort Worth, TX, 2009.
  9. CITI training for human subject research (2007) and refresher course on biomedical and clinical research (2009, 2011, 2012, 2013).
  10. STAR fellowship program of Texas Center for Health Disparities, Fort Worth, TX 2007 – 2008.
  11. Workshop on Grant Writing at UNT Health Science Center, Fort Worth, TX, 2007.
  12. Training for the care and use of laboratory animals at UNT Health Science Center, Fort Worth, TX, 2003.
  13. Radiation safety training, UNT Health Science Center, Fort Worth, TX, 2002.

 

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