Publications

Published 84 internationally-refereed papers in journals such as Journal of Biological Chemistry, Journal of Bacteriology, Journal of Clinical Microbiology, Molecular General Genetics, Gene, Plasmid, FEMS Letters, Genitourinary Medicine, Epidemiology & Infection, Applied & Environmental Microbiology, Antimicrobial Agents & Chemotherapy, Virology, Proteomics, PLoS One, Vaccine, Virus
Research, Journal of Virology and Antiviral Research.
I have co-authored three chapters in books. Two were in books published by ASM Press. In “Pseudomonas-Molecular Biology and Biotechnology” Edited by Enrica Galli, Simon Silver & Berhard Witholt , 1992.
I have contributed a chapter entitled ”Detection of Enteroviruses” in “Dignostic Virology Protocols” published by Humana Press in January, 2011 as the main author.
I have been invited to contribute a chapter entitled” Peptide Antibodies” to be published by Humana Press in 2015.

Presented 45 Internationally-refereed conference papers.

Publications:

  1. Tan CW, Chan YF, Quah YW , Poh CL. Inhibition of Enterovirus 71 infection by antisense octaguanidinium   dendrimer-conjugated morpholino oligomers. Antiviral Research 2014  July, 107: 35-41.
  2. Lazouskaya NV, Palombo EA, Poh CL, Barton PA.  Construction of an infectious cDNA clone of Enterovirus 71: Insights  into the factors ensuring experimental success. J Virol Methods 2014  Mar, 197: 67-76
  3. Lui YL, Lin Z, Lee JJ, Chow VT, Poh CL, Tan EL. Beta-actin variant is necessary for Enterovirus 71 replication. Biochem Biopys Res Commun.2013 April, 433(4): 607-610.
  4. Tan CW, Poh CL, Sam IC and Chan YF.  Enterovirus 71 uses cell surface heparin sulfate glycosaminoglycan as  an attachment receptor. J Virology 2013 Jan, 87(1): 611-20.
  5. Kirk K, Poh CL, Fecondo J, Pourianfar H, Shaw J and Grollo L. Cross-reactive neutralizing antibody epitopes against Enterovirus 71  identified by an in silico approach. Vaccine 2012 Sept, 30(49):  7105-7110.
  6. Pourianfar HR, Poh CL, Fecondo J and Grollo L. In vitro evaluation of the antiviral activity of heparin sulfate   compounds against Enterovirus 71. Virus Research. 2012 July, 169: 22-29.
  7. Tan CW, Chan YF, Sim KM, Tan EL and Poh CL. Inhibition of Enterovirus 71(EV-71) infections by a novel antiviral peptide derived from EV-71 capsid protein VP1. PLoS One 2012 May 7(5); 1-11.
  8. Lee LL, Seah JB, Chow VT, Poh, CL and Tan EL. Comparative proteome analysis of host protein expression in respons to Enterovirus 71 and Coxsackievirus A16 infections.  J Proteomics. 2011 Sep, 74(10): 2018-2024.
  9. Poh CL and Tan EL. Detection of enteroviruses from clinical specimens. Methods Mol Biol. 2011 Jan, 665:65-77.
  10. Wu Y, Tan EL, Yeo A, Cahn KP, Nishimura H, Cardosa MJ, Poh CL, Quak SH, Chow VT. Novel multiplex oligonucleotide-conjugated bead suspension array for  rapid identification of enterovirus 71 subgenogroups. J Clin Microbiol. 2011 Jan, 49(1): 419-422.
  11. Kurupati P, Ramachandran NP, Poh CL. Protective efficacy of DNA vaccines encoding outer membrane protein  A and OmpK36 of Kelbsiella pneumonia in mice. Clin Vaccine Immunol. 2011 Jan, 18(1): 82-88.
  12. Wu Y, Yeo A, Phoon MC, Tan EL, Poh CL, Quak SH, Chow VT.  The largest outbreak of hand, foot and mouth disease in Singapore in 2008: the role of enterovirus 71 and coxsackievirus A strains. Int J Infect Dis. 2010 Dec, 14(12): 1076-1081.
  13. Tan EL, Wong AP,  Poh CL. Development of potential antiviral strategy against coxsackievirus  B4. Virus research. 2010 Jun, 150: 85-92.
  14. Tan EL, Marcus KF, Poh CL. Development of RNA interference (RNAi) as potential antiviral   strategy against enterovirus 70. J Med Virol. 2008 Jun, 80(6):1025-1032.
  15. Foo  DG, Macary PA, Alonso S, Poh CL. Identification of Human CD4(+) T-cell epitopes on the VP1 capsid protein of Enterovirus 71.  Viral Immunol. 2008 Jun, 21(2): 215-224.
  16. Tan EL, Chow VT, Quak SH, Yeo WC, Poh CL. Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection  and differentiation of Enterovirus 71 and Coxsackievirus A16. Diag Microbiol Infect Dis 2008 Jul, 61(3):294-301.
  17. Tan EL, Yong LL, Quak SH, Yeo WC, Chow VT, Poh CL. Rapid detection of  Enterovirus 71 by real-time TaqMan RT- PCR. J Clin Virol 2008 Jun, 42(2): 203-206.
  18. Zhao B, Poh CL. Insights into environmental bioremediation by microorganisms through functional genomics and proteomics. Proteomics 2008  Feb, 8(4): 874-881.
  19. Foo DG, Ang RX, Alonso S, Chow VT, Quak SH, Poh CL. Identification of immunodominant VP1 linear epitope of enterovirus 71( EV71) using synthetic peptides for detecting  human anti-EV71 IgG antibodies in western blots.  Clin Microbiol Infect. 2008 Mar, 14(3): 286-288. 
  20. Ho SY, Chua SQ, Foo DG, Locht C, Chow VT, Poh CL, Alonso S. The highly attenuated Bordetella pertussis BPZE1 strain as a potential vehicle for the delivery of heterologous vaccine  candidates. Infect Immun. 2008, Jan, 76(1): 111-119.
  21. Foo DG, Alonso S, Chow VT, Poh CL. Passive protection against lethal enterovirus 71 infection in new born mice by neutralizing antibodies elicited by a synthetic peptide. Microbes & Infect.  2007 Sep , 9: 1299-1306.
  22. Yeo CC, Tan CL, Gao X, Zhao B, Poh CL. Characterization of hbzE-encoded gentisate 1, 2-dioxygenase from Pseudomonas alcaligenes  NCIMB 9867. Res Microbiol. 2007 Sep, 158: 608-16.
  23. Tan EL, Tan TM, Chow VT, Poh CL. Inhibition of Enterovirus 71 in virus-infected mice by RNA Interference. Mol Ther. 2007 Nov, 15: 1070-85.
  24. Tan EL, Tan TM, Chow VT, Poh CL. Enhanced potency and efficacy of 29-mer shRNAs in inhibition of  Enterovirus 71. Antiviral Res.  2007 Apr, 74: 9-15.
  25. Kurupati P, Tan KS, Kumarasinghe G, Poh CL. Inhibition of gene expression and growth by antisense peptide nucleic acids in a multiresistant beta-lactamase-producing Klebsiella pneumonia strain. Antimicrob Agents Chemother. 2007 Mar, 51:805-11.
  26. Kurupati P, Teh BK, Kumarasinghe G, Poh CL. Identification of vaccine candidate antigens of an ESBL  producing Klebsiella pneumoniae  clinical strain by immunoproteome analysis. Proteomics.2006 Feb, 6: 836-44
  27. Kurupati P, Kumarasinghe G, Poh CL. Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme linked immunosorbent assay  using oprI gene specific primers. Mol Cell Probes. 2005 Nov; 19:417-21.
  28. Gao X, Tan CL, Yeo CC, Poh CL. Molecular and biochemical characterization of the xlnD  encoded 3-hydroxybenzoate 6-hydroxylase involved in the radation of 2,5-xylenol via the gentisate pathway in  Pseudomonas alcaligenes NCIMB 9867. J Bacteriol. 2005 Nov; 187(22): 7696-702.
  29. Tan CL, Yeo CC, Khoo HE, Poh CL. Replacement of tyrosine 181 by phenylalanine in gentisate 1,2- dioxygenase I from Pseudomonas alcaligenes NCIMB 9867 enhances catalytic activities. J Bacteriol. 2005 Nov; 187(21): 7543-5.
  30. Sim AC, Luhur A, Tan TM, Chow VT, Poh CL. RNA interference against Enterovirus 71 infection. Virology. 2005 Oct,  341:72-79. 
  31. Zhao B, Yeo CC, Poh CL. Proteome investigation of the global regulatory role of sigma54 in response to gentisate induction in Pseudomonas alcaligenes  NCIMB 9867. Proteomics. 2005 May; 5(7):1868-76.
  32. Sachidanandham R, Gin KY, Poh CL. Monitoring of active but non-culturable bacterial cells by flow    cytometry. Biotechnol Bioeng. 2005 Jan 5; 89(1):24-31.
  33. Zhao B, Yeo CC, Lee CC, Geng A, Chew FT, Poh CL. Proteome analysis of gentisate-induced response in   Pseudomonas alcaligenes NCIB 9867. Proteomics. 2004 Jul; 4(7):2028-36.
  34. Ranganathan S, Singh S, Poh CL, Chow VT. The hand, foot and mouth disease virus capsid: sequence        analysis and prediction of antigenic sites from homology modelling. Appl Bioinformatics. 2002; 1(1):43-52.
  35. Kurupati P, Chow C, Kumarasinghe G, Poh CL. Rapid detection of Klebsiella pneumoniae from blood culture  bottles by real-time PCR. J Clin Microbiol. 2004 Mar; 42(3):1337-40
  36. Yeo CC, Wong MV, Feng Y, Song KP, Poh CL. Molecular characterization of an inducible gentisate 1,2- dioxygenase gene, xlnE, from Pseudomonas alcaligenes  NCIMB 9867. Gene. 2003 Jul 17;  312: 239-48.
  37. Teo JW, Zhang LH, Poh CL.Cloning and characterization of a novel lipase from Vibrio  harveyi strain AP6. Gene. 2003  Jul 17; 312:181-8.
  38. Teo JW, Zhang LH, Poh CL. Cloning and characterization of a metalloprotease from Vibrio  harveyi strain AP6. Gene. 2003   Jan 16; 303:147-56.
  39. Singh S, Poh CL, Chow VT Complete sequence analyses of enterovirus 71 strains from fatal and non-fatal cases of the hand, foot and mouth disease outbreak in Singapore (2000). Microbiol  Immunol. 2002; 46(11):801-8.
  40. Singh S, Chow VT, Phoon MC, Chan KP, Poh CL. Direct detection of enterovirus 71 (EV71) in clinical specimens  from a hand, foot, and mouth disease outbreak in Singapore by reverse transcription-PCR with universal enterovirus and EV71- specific primers. J Clin Microbiol. 2002 Aug; 40(8):2823-7.
  41. Teo JW, Tan TM, Poh CL. Genetic determinants of tetracycline resistance in Vibrioharveyi. Antimicrob Agents Chemother. 2002 Apr; 46(4):1038-45.
  42. Chua CH, Feng Y, Yeo CC, Khoo HE, Poh CL. Identification of amino acid residues essential for catalytic activity of gentisate 1,2-dioxygenase from Pseudomonas alcaligenes NCIB 9867. FEMS Microbiol Lett. 2001 Oct 16; 204(1):141-6.
  43. Yeo CC, Yiin S, Tan BH, Poh CL. Isolation and characterization of group II introns from Pseudomonas alcaligenes and Pseudomonas putida. Plasmid. 2001 May; 45(3):233-9.
  44. Kwong SM, Yeo CC, Poh CL. Molecular analysis of the pRA2 partitioning region: ParB autoregulates parAB transcription and forms a nucleoprotein  complex with the plasmid partition site, parS. Mol Microbiol. 2001 May; 40(3):621-33.
  45. Singh S, Chow VT, Chan KP, Ling AE, Poh CL. RT-PCR, nucleotide, amino acid and phylogenetic analyses of  enterovirus type 71 strains from Asia. J Virol Methods. 2000 Aug; 88(2):193-204.
  46. Soh SW, Poh CL, Lin RV. Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolates from pediatric patients in Singapore. Antimicrob Agents Chemother. 2000 Aug; 44(8):2193-6.
  47. Norman RA, Poh CL, Pearl LH, O'Hara BP, Drew RE. Stearic hindrance regulation of the Pseudomonas aeruginosa  amidase operon. J Biol Chem. 2000 Sep 29; 275(39):30660-7.
  48. Teo JW, Suwanto A, Poh CL. Novel beta-lactamase genes from two environmental isolates of Vibrio harveyi. Antimicrob Agents Chemother. 2000 May; 44(5):1309-14.
  49. Kwong SM, Yeo CC, Suwanto A, Poh CL. Characterization of the endogenous plasmid from Pseudomonas alcaligenes NCIB 9867: DNA sequence and mechanism of  transfer. J Bacteriol. 2000 Jan; 182(1):81-90.
  50. Feng Y, Khoo HE, Poh CL. Purification and characterization of gentisate 1, 2-dioxygenases from Pseudomonas alcaligenes NCIB 9867 and Pseudomonas putida NCIB 9869. Appl Environ Microbiol. 1999 Mar; 65(3):946-50.
  51. Xu SY, Xiao JP, Ettwiller L, Holden M, Aliotta J, Poh CL,  Dalton M, Robinson DP, Petronzio TR, Moran L, Ganatra M, Ware J, Slatko B, Benner J. Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli. Mol Gen Genet. 1998 Nov; 260(2-3):226-31.
  52. Yeo CC, Tham JM, Kwong SM, Poh CL. Characterization of the Pac25I restriction-modification genes isolated from the endogenous pRA2 plasmid of Pseudomonas  alcaligenes NCIB 9867. Plasmid. 1998 Nov; 40(3):203-13.
  53. Yeo CC, Tham JM, Kwong SM, Yiin S, Poh CL. Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes. FEMS Microbiol Lett. 1998 Aug 15; 165(2):253-60.
  54. Yeo CC, Wong DT, Poh CL. IS1491 from Pseudomonas alcaligenes NCIB 9867: characterization and distribution among Pseudomonas species. Plasmid. 1998; 39(3):187-95.
  55. Kwong SM, Yeo CC, Chuah D, Poh CL. Sequence analysis of plasmid pRA2 from Pseudomonas alcaligenes NCIB 9867 (P25X) reveals a novel replication  region. FEMS Microbiol Lett. 1998 Jan 15; 158(2):159-65.
  56. Ng DP, Goh KT, Yeo MG, Poh CL. An institutional outbreak of Salmonella enteritidis in Singapore. Southeast Asian J Trop Med Public Health. 1997 Mar; 28(1):85-90.
  57. Yeo CC, Tham JM, Yap MW, Poh CL. Group II intron from Pseudomonas alcaligenes NCIB 9867  (P25X): entrapment in plasmid RP4 and sequence analysis. Microbiology. 1997 Aug; 143 ( Pt 8):2833-40.
  58. Yeo CC, Poh CL. Characterization of IS1474, an insertion sequence of the IS21  family isolated from Pseudomonas alcaligenes NCIB 9867. FEMS Microbiol Lett. 1997 Apr 15; 149(2):257-63.
  59. Yeo CC, Poh CL. IS1394 from Pseudomonas alcaligenes N.C.I.B. 9867:  identification and characterization of a member of the IS30 family of insertion elements. Gene. 1996 Oct 10; 175(1-2):109-13.
  60. Poh CL, Ramachandran V, Tapsall JW. Genetic diversity of Neisseria gonorrhoeae IB-2 and IB-6  isolates revealed by whole-cell repetitive element sequence- based PCR. J Clin Microbiol. 1996 Feb; 34(2):292-5.
  61. Lau QC, Chow VT, Poh CL. Differentiation of Neisseria gonorrhoeae strains by polymerase chain reaction and restriction fragment length polymorphism of outer membrane protein IB genes. Genitourin Med. 1995 Dec; 71(6):363-6.
  62. Poh CL, Lau QC, Chow VT. Differentiation of Neisseria gonorrhoeae IB-3 and IB-7  serovars by direct sequencing of protein IB gene and pulsed- field gel electrophoresis. J Med Microbiol. 1995 Sep; 43(3):201-7.
  63. Poh CL, Loh GK, Tapsall JW. Resolution of clonal subgroups among Neisseria gonorrhoeae IB-2 and IB-6 serovars by pulsed-field gel electrophoresis. Genitourin Med. 1995 Jun; 71(3):145-9.
  64. Ng LC, Poh CL, Shingler V. Aromatic effector activation of the NtrC-like transcriptional regulator PhhR limits the catabolic potential of the (methyl)phenol degradative pathway it controls.  Bacteriol. 1995 Mar;177 (6):1485-90.
  65. Ng LC, Shingler V, Sze CC, Poh CL. Cloning and sequences of the first eight genes of the chromosomally encoded (methyl) phenol degradation pathway from Pseudomonas putida P35X. Gene. 1994 Dec 30; 151(1-2):29-36.
  66. Nair S, Poh CL, Lim YS, Tay L, Goh KT. Genome fingerprinting of Salmonella typhi by pulsed-field gel electrophoresis for subtyping common phage types. Epidemiol Infect. 1994 Dec; 113(3):391-402.
  67. Lau QC, Chow VT, Poh CL. Polymerase chain reaction and direct sequencing of Neisseria gonorrhoeae protein IB gene: partial nucleotide and amino acid sequence analysis of strains S4, S11, S48 (serovar IB4) and S34  (serovar IB5). Med Microbiol Immunol (Berl). 1993 Jul; 182(3):137-45.
  68. Poh CL, Yeo CC. Recent advances in typing of Pseudomonas aeruginosa. J Hosp Infect. 1993 Jul; 24(3):175-81.
  69. Poh CL, Yap SC, Yeo M. Pulsed-field gel electrophoresis for differentiation of hospital isolates of Klebsiella pneumoniae. J Hosp Infect. 1993 Jun; 24(2):123-8.
  70. Poh CL, Lau QC. Subtyping of Neisseria gonorrhoeae auxotype-serovar groups by pulsed-field gel electrophoresis. J Med Microbiol. 1993 May; 38(5):366-70.
  71. Assinder SJ, De Marco P, Osborne DJ, Poh CL, Shaw LE, Winson MK, Williams PA. A comparison of the multiple alleles of xylS carried by TOL plasmids pWW53 and pDK1 and its implications for their evolutionary relationship.J Gen Microbiol. 1993 Mar; 139(3):557-68.
  72. Poh CL, Yeo CC, Tay L. Genome fingerprinting by pulsed-field gel electrophoresis and  ribotyping to differentiate Pseudomonas aeruginosa serotype O11 strains. Eur J Clin Microbiol Infect Dis. 1992 Sep; 11(9):817-22.
  73. Poh CL, Khng HP, Lim CK, Loh GK. Molecular typing of Neisseria gonorrhoeae by restriction fragment length polymorphisms. Genitourin Med. 1992 Apr; 68(2):106-10.
  74. Poh CL, Ocampo JC, Loh GK. Genetic relationships among Neisseria gonorrhoeae serovars analysed by multilocus enzyme electrophoresis. Epidemiol Infect. 1992 Feb; 108(1):31-8.
  75. Poh CL, Ocampo JC, Sng EH, Bygdeman SM. Characterisation of PPNG and non-PPNG Neisseria gonorrhoeae isolates from Singapore. Genitourin Med. 1991 Oct; 67(5):389-93.
  76. Poh CL, Ocampo JC, Sng EH, Bygdeman SM. Rapid in situ generation of DNA restriction endonuclease patterns for Neisseria gonorrhoeae. J Clin Microbiol. 1989 Dec; 27(12):2784-8.
  77. Poh CL, Bayly RC. Regulation of isofunctional enzymes in Pseudomonas alcaligenes mutants defective in the gentisate pathway. J Appl Bacteriol. 1988 May; 64(5):451-8.
  78. Poh CL, Loh GK. Enzymatic characterization of Pseudomonas cepacia by API ZYM profile. J Clin Microbiol. 1988 Mar; 26(3):607-8.
  79. Poh CL, Yap EH, Tay L, Bergan T. Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa. J Med Microbiol. 1988 Feb; 25(2):109-14.
  80. Bergan T, Lolekha S, Cheong MK, Poh CL, Doencham S, Charoenpipop D. Effect of recent antibacterial agents against bacteria causing  diarrhoea. Scand J Infect Dis Suppl. 1988; 56:7-10.
  81. Bergan T, Lolekha S, Cheong MK, Poh CL, Patancharoen S. Consequences of diarrhoeal disease on the pharmacokinetics of norfloxacin. Scand J Infect Dis Suppl. 1988; 56:11-3.
  82. Poh CL, Yap EH, Tay L, Loh GK, Thong TW. Predominance of Pseudomonas aeruginosa serotype 11, pyocin types 1 and 10 in Singapore. Singapore Med J. 1986 Apr; 27(2):135-9.
  83. Poh CL, Loh GK. Enzymatic profile of clinical isolates of Acinetobacter calcoaceticus. Med Microbiol Immunol (Berl). 1985; 174(1):29-33.
  84. Poh CL, Bayly RC. Evidence for isofunctional enzymes used in m-cresol and 2,5-xylenol degradation via the gentisate pathway in Pseudomonas alcaligenes. J Bacteriol. 1980 Jul; 143(1):59-69.

 

 

 


Chit Laa Poh, PhD

Distinguished Professor of Biological Sciences

 

  • : +60-162363293
  • Department
    Sunway University
  • COUNTRYMALAYSIA