Publications

Peer-reviewed Articles

  1. Zhang, X., Chen, SZ, and Zhao, SY. The Gene Structure of Ciliary Neurotrophic Factor and its Receptor. (1996) Foreign Med. Sci. (Molecular Biology) 18(5):225-9.
  2. Zhang, X., Ma, B., Shu, N., Chen, SZ, and Zhao, SY. Cloning the Gene Coding for Human Glial Cell Line-derived Neurotrophic Factor and its Expression in Escherichia Coli. Chinese Journal of Biotechnology (1997) 13(4): 426-9.
  3. Zhang, X., Micheli, M. De., Coleman, S., Sanglard, D., Moye-Rowley, WS. Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p. Molecular Microbiology (2000) 36(3): 618-629.
    Citation: 111
  4. Zhang, X., Cui, Z. Miyakawa, T. Moye-Rowley, WS. Crosstalk between transcriptional regulators of multidrug resistance in Saccharomyces cerevisiae. Journal of Biological Chemistry (2001) 276(12): 8812-9.
    Citation: 42
  5. Zhang, X., Moye-Rowley, WS.  Saccharomyces cerevisiae multidrug resistance gene expression inversely correlates with the status of the F(0) component of the mitochondrial ATPase.  Journal of Biological Chemistry (2001) 276(51): 47844-47852.
    Citation: 58
  6. Le Crom, S., Devaux, F., Marc, P., Zhang, X., Moye-Rowley WS. and Jacq, C. New insights into the Pleiotropic Drug Resistance network from genome-wide characterization of YRR1 transcription factor regulation system. Molecular and Cellular Biology (2002) 22(8): 2642-2649.
    Citation: 86
  7. Zhang X, Kolaczkowska A, Devaux F, Panwar SL, Hallstrom TC, Jacq C, Moye-Rowley WS. Transcriptional regulation by Lge1p requires a function independent of its role in histone H2B ubiquitination. Journal of Biological Chemistry (2005) 280(4):2759-70.
    Citation: 32
  8. Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait B and Roeder RG. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for estrogen receptor-mediated transcription. Molecular Cell (2005) 19:89-100.
    Citation: 88
  9. Stumpf M, Waskow C, Krötschel M, Essen D, Rodriguez P, Zhang X, Guyot B, Roeder RG and Borggrefe T. The Mediator Complex Functions as a Coactivator for GATA1 in Erythropoiesis via Subunit Med1/TRAP220. Proc. Natl. Acad. Sci. (2006) 103:18504-9.

Paper Published Since Joining UC

  1. Jiang P, Hu Q, Ito M, Meyer S, Waltz S, Khan S, Roeder RG and Zhang X.  Key Role of MED1/TRAP220 LxxLL Motifs in Pubertal Mammary Gland Development and Luminal-Cell Differentiation. Proc. Natl. Acad. Sci. U S A (2010) 107:6765-6770. PMCID: PMC2872411 (Corresponding Author)
    Citation: 42
  2. Chen W*, Zhang X*, Birsoy K, and Roeder RG.  A muscle-specific knockout implicates nuclear receptor coactivator MED1 in the regulation of glucose and energy metabolism. Proc Natl Acad Sci U S A. (2010) Jun 1;107(22):10196-201. PMCID: PMC2890439 (*co-first author).
    Citation: 27
  3. Pfaff D, Waters J,  Kahn I, Zhang X and Numan M. (Invited Review) Estrogen receptor-initiated mechanisms causal to mammalian reproductive behaviors. Endocrinology. (2011) Apr; 152(4): 1209-17. PMCID: PMC3060638
    Citation: 16
  4. Zhang D, Jiang P, Xu Q, and Zhang X. ARGLU1 interacts with MED1 and is required for estrogen receptor-mediated transcription and breast cancer cell growth. Journal of Biological Chemistry. (2011) May 20; 286(20):17746-54. Epub 2011 Mar 28. (Corresponding Author)
    Citation: 14
  5. Chen Z, Zhang C, Wu D, Rorick A, Zhang X, and Wang Q. Phospho-MED1-enhanced UBE2C locus looping drives castration-resistant prostate cancer growth. EMBO J. (2011) May 10; 30(12):2405-19.
    Citation: 48
  6. Cui J, Germer K, Wu T, Wang J, Luo J, Wang SC, Wang Q, and Zhang X. Crosstalk Between HER2 and MED1 Regulates Tamoxifen Resistance of Human Breast Cancer Cells. Cancer Research (2012) 72:5625-5634. (Corresponding Author)
    Citation: 23
  7. M Czyzyk-krzeska and Zhang X. MiR-155 at the Heart of Oncogenic Pathways. (Commentary) Oncogene. (2013) Advance online publication, 18 February 2013; doi:10.1038/onc.2013.26
    Citation: 11
  8. Germer K, Leonard M, and Zhang X. RNA aptamer and its diagnostic and therapeutic applications. (2013) Int J Biochem Mol Biol. 2013 Mar 31; 4(1):27-40. (Corresponding Author)
    Citation: 15
  9. Germer K, Pi M, Guo P and Zhang X. Conjugation of RNA aptamer to pRNA nanoparticles for RNA-based therapy. (2013) RNA Nanotechnology and Therapeutics, CRC Press 2013 July. Pages: 399-408 (Corresponding Author)
    Citation: 2
  10. Zhang L, Cui J, Leonard M, Nephew K, Li Y, and Zhang X. Silencing MED1 Sensitizes Breast Cancer Cells to Anti-estrogen Fulvestrant Therapy in vitro and in vivo. (2013) PLoS ONE 8(7): e70641. doi:10.1371/journal.pone.0070641 (*Corresponding Author)
    Citation: 1
  11. Leonard M, Zhang Y and Zhang X. Small non-coding RNAs and aptamers in diagnostics and therapeutics. (Invited Book Chapter) (2014) Methods in Molecular Biology. Springer Protocols.

Manuscript In Preparation:

  1. Jiang P, Xu Q, Hu Q, and Zhang X. MED1 regulates cellular senescence through p16/pRB/E2F1 pathway and the recruitment of SUV39H1 and G9a. (Corresponding Author, Manuscript in Preparation)
  2. Yang Y, Leonard M, Zhang L, Gu H, Meredith A and Zhang X. MED1 LxxLL motifs are required for MMTV-PyVT tumor progression, metastasis and cancer stem cell formation in vivo. (Corresponding Author, Manuscript in Preparation)
  3. Zhang L and Zhang X. MED1 Promotes ER-positive breast cancer cell invasion and metastasis through regulating MMP9. (Corresponding Author, Manuscript in Preparation)

Quality Review of Publications

  1. Zhang, X., Micheli, M. De., Coleman, S., Sanglard, D., Moye-Rowley, WS. Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p. Molecular Microbiology (2000) 36(3): 618-629.
    In this paper, I found that Cap1p plays a major role in mediating the oxidative stress response of the pathogenic fungus Candida albicans. This study provided novel insights into molecular mechanism of drug resistance in pathogenic fungi, and potential therapeutic targets for their treatment. I am the first author, and was responsible for the design and execution of most of the experiments described.
    Total Citations (90), 2012(2), 2011(4), 2010(8), 2009(10), 2008(5)
  2. Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait B and Roeder RG. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for estrogen receptor-mediated transcription. Molecular Cell (2005) 19:89-100.
    This study showed that the MED1 subunit of the Mediator complex is required for estrogen-dependent transcription and breast cancer cell growth.  Further biochemical analysis revealed that MED1 is present only in a subpopulation of Mediator complexes, indicating a previously unexpected heterogeneity of the Mediator complex.  Significantly, we found this MED1/Mediator complex has a unique subunit composition, with 7 additional new Mediator subunits and enriched RNA polymerase II.  I am the first author, and was responsible for the design and execution of most of the experiments described.
    Total Citations (69), 2012(6), 2011(8), 2010(11), 2009(3), 2008(7)
  3. Jiang P, Hu Q, Ito M, Meyer S, Waltz S, Khan S, Roeder RG and Zhang X.  Key Role of MED1/TRAP220 LxxLL Motifs in Pubertal Mammary Gland Development and Luminal-Cell Differentiation. Proc. Natl. Acad. Sci. U S A (2010) 107:6765-6770. PMCID: PMC2872411
    This is the first paper to investigate the role and importance of the nuclear receptor/cofactor interaction in vivo.  Surprisingly, we found that MED1 nuclear-receptor-interacting LxxLL motifs play critical in vivo tissue- and cell-specific roles in mediating estrogen receptor functions in mammary gland.  Significantly, we discovered that MED1 is differentially expressed in mammary epithelial cells (in contrast to the current assumption that Mediator subunits are universally expressed), and is required for luminal cell progenitor/stem cell determination.  I am the last/corresponding author of the paper, and was responsible for the design, execution, and coordination for the completion of this project.
    Total Citations (22), 2012(9), 2011(11), 2010(2)
  4. Zhang D, Jiang P, Xu Q, and Zhang X. ARGLU1 interacts with MED1 and is required for estrogen receptor-mediated transcription and breast cancer cell growth. Journal of Biological Chemistry. (2011) May 20; 286(20):17746-54. Epub 2011 Mar 28. (Corresponding Author)
    This paper is the first paper describing the function of a previously unpublished protein ARGLU1 (arginine and glutamate rich 1). We found ARGLU1 to be a novel MED1/Mediator-associated protein that functions synergistically with MED1 to regulate estrogen receptor-mediated transcription. We provided further mechanistic insights into ARGLU1 structural function relationships by using deletion mapping, ChIP and ChIP-re-ChIP assays etc. Importantly, depletion of ARGLU1 significantly impairs the estrogen-dependent gene expression and growth of breast cancer cells. I am the last/corresponding author of the paper, and was responsible for the design, execution, and coordination for the completion of this project.
    Total Citation: 5
  5. Cui J, Germer K, Wu T, Wang J, Luo J, Wang SC, Wang Q, and Zhang X. Crosstalk Between HER2 and MED1 Regulates Tamoxifen Resistance of Human Breast Cancer Cells. Cancer Research (2012) doi: 10.1158/0008-5472 (Corresponding Author)
    This important study defines an estrogen receptor co-activator that plays a central role in mediating tamoxifen resistance in human breast cancer, addressing a widespread issue arising in the management of ERα-positive breast cancer patients. Despite the fact that most breast cancer patients have ERα-positive tumors, up to 50% of the patients are or soon develop resistance to endocrine therapy. It is recognized that HER2 activation is one of the major mechanisms contributing to endocrine resistance. In this study, we report that the estrogen receptor coactivator MED1 is a novel crosstalk point for the HER2 and ERα pathways in mediating endocrine resistance and indicate that targeting MED1 could be a useful strategy in fighting against endocrine resistance by simultaneously blocking both pathways. I am the last/corresponding author of the paper, and was responsible for the design and coordination for the completion of this project.
    Total Citation: N/A

Abstracts

  1. Zhang, X., Cui, Z., Miyakawa, T., and Moye-Rowley, WS.  Multiple Cys6 zinc cluster transcription factors regulate multidrug resistance in Saccharomyces cerevisiae. Yeast Genetics and Molecular Biology Meeting, College Park, MD, August, 1998.
  2. Zhang, X., Cui, Z., Miyakawa, T., and Moye-Rowley, WS.  Regulation and function of the zinc cluster transcription factor Yrr1p. Yeast Genetics and Molecular Biology Meeting, Seattle, WA, August, 2000.
  3. Marc, P., Devaux, F., Le Crom, S., Hikkel, I., Zhang, X., Moye-Rowley, S., Jacq, C. Regulations of the transcriptome adaptations to cellular environment: the yeast multidrug resistance phenomenon. The fifth annual international conference on computational molecular biology, Montreal, Canada, April, 2001.
  4. Zhang, X, Hallstrom T and Moye-Rowley, WS.  Fo status regulates multidrug resistance in S. cerevisiae. (Platform Presentation) Yeast Cell Biology Meeting, Cold Spring Harbor, NY, August 2001.
  5. Zhang, X., Kolaczkowska, A., Tekippe, J., Moye-Rowley, S.  Ypl055cp functions downstream from Pdr3p during retrograde activation of PDR5 transcription. Yeast Genetics and Molecular Biology Meeting, Madison WI, August, 2002.
  6. Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait B and Roeder RG. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation and is required for estrogen receptor-mediated transcription and breast cancer cell growth. 4th annual future of breast cancer conference, Fairmont hotel, Bermuda, July 2005.
  7. Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait B and Roeder RG. A MED1/TRAP220-containing TRAP/Mediator subpopulation and its role in estrogen receptor-mediated transcription. Eukaryotic Transcription Mechanism, Cold Spring Harbor, NY, August 2005.
  8. Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait B and Roeder RG. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for estrogen receptor-mediated transcription. (Platform Presentation) EMBO Conference: Nuclear receptors: from chromatin to disease, Lake Garda, Italy, September 2005.
  9. Zhang X. and Roeder RG. MED1/TRAP220-containing TRAP/Mediator and its role in ER-mediated functions. (Platform Presentation) Komen Mission Conference: Many Faces – One Voice, Washington D.C. U.S.A., June 2006.
  10. Asheena Keith & Xiaoting Zhang.  Analyses of MED1/TRAP220 and its Associated Protein Subunits. 2007 Annual Biomedical Research Conference for Minority Students (ABRCMS) Nov. 7-11, 2007, Austin, TX, USA
  11. Jiang P, Hu Q, Ito M, Roeder RG and Zhang X.  Role of Transcriptional Coactivator MED1/TRAP220 in Mammary Gland Development. IUBMB conference, Aug. 2-7, 2009, Shanghai, China
  12. Hu Q, Jiang P, Meyer S, Waltz S, Khan S, Ito M, Roeder RG and Zhang X.  Critical Role of MED1/TRAP220 Minimal Nuclear Receptor-Interacting LxxLL Motifs in Mammary Gland Development. Jensen Symposium in Nuclear Receptor, Oct. 14-16, 2009, Cincinnati, OH, USA
  13. Jiang P, Hu Q, Ito M, Meyer S, Waltz S, Khan S, Roeder RG and Zhang X. Tissue-Specific Roles of MED1/TRAP220 Nuclear Receptor-Interacting LxxLL Motifs in vivo. ASBMB annual meeting, Apr. 24-28, 2010, Anaheim, CA, USA
  14. Jiang P, Hu Q, Zhang D, and Zhang X. Nuclear Receptor Cofactor MED1 Regulates Glucose Metabolism and Energy Homeostasis in Muscle. Diabetes and Obesity Center of Excellence Research Retreat. June, 2010. Cincinnati, OH, USA (Awarded Second Place Prize).
  15. Jiang P, Hu Q, Ito M, Meyer S, Waltz S, Khan S, Roeder RG and Zhang X. Tissue-Specific Roles of Transcriptional Coregulator MED1/TRAP220 in vivo. CSHL Nuclear Receptor Meeting, Aug. 31- Sep. 4, 2010, CSHL, NY, USA
  16. Zhang D, Jiang P, Xu, Q, and Zhang X.  ARGLU1 interacts with MED1 and is required for estrogen receptor-mediated transcription and breast cancer cell growth. Cincinnati Cancer Symposium, May. 1-4, 2011, Cincinnati, OH
  17. Jiang P, Zhang D, Xu, Q, Meeks, J, Zhang, L and Zhang X.  Transcriptional Cofactor MED1 in Mammary Stem/Progenitor Cell Determination and Breast Cancer. (Platform Presentation) Zing Cancer Conference 2011--- From Carcinogenesis to Cancer Therapy, Nov. 9-13, 2011, Occidental Grand Xcaret, Mexico
  18. Zhang D, Jiang P, Xu, Q, and Zhang X. Key Roles of Transcriptional Coactivator MED1 in Breast Cancer Cell Growth. (Session Chair and Speaker, Stem cell and Breast Cancer), BIT's 4th Annual World Cancer Congress-Breast Cancer Conference 2011, Nov. 15-18, 2011, Guangzhou, China
  19. Cui J, Zhang D, Jiang P, Xu Q, and Zhang X. Estrogen receptor coactivator MED1 in mammary progenitor/stem cell determination and tamoxifen resistance of breast cancer. (Platform Presentation) AACR Annual Meeting 2012, Mar. 31-Apr. 4, 2012, Chicago, IL.
  20. Cui J, Jiang P, Xu Q, Wang Q and Zhang X. A positive feedback loop between HER2 and MED1 regulates tamoxifen resistance of breast cancer. Keystone Symposia: Nuclear Receptor Matrix: Reloaded, Apr. 15-21, 2012, Whistler, Canada.
  21. Cui J, Germer K, Wu T, Wang J, Luo J, Wang SC, Wang Q, and Zhang X. HER2/MED1 Crosstalk and Tamoxifen Resistance of Human Breast Cancer Cells. UC Health Research Week 2012: Collaborate to Innovate, Nov. 13-16, Cincinnati, OH
  22. Leonard M and Zhang X. The Development and Use of RNA Aptamers to Target MED1/ER Interaction. University of Cincinnati Undergraduate Research Symposium, Dec. 14, 2012, Cincinnati, OH
  23. Bauer-Nilsen K, Leonard M, Cui J, Wu T, Wang J, Chen C, Guo P and Zhang X. Estrogen Receptor Coactivator MED1 in Endocrine Resistance and as a Therapeutic Target for Human Breast Cancer. 2nd International Conference of RNA Nanotechnology and Therapeutics. Apr. 4-6, 2013. Lexington, KY
  24. Meredith A, Cui J and Zhang X. Identification of ARGLU1 interacting proteins with a golden yeast two hybrid system. University of Cincinnati Undergraduate Research Symposium, Apr. 19, 2013, Cincinnati, OH
  25. Bauer-Nilsen K and Zhang X. Targeting estrogen receptor coactivator MED1 to overcome tamoxifen resistance using RNA nanotechnology. University of Cincinnati Undergraduate Research Symposium, Apr. 19, 2013, Cincinnati, OH
  26. Yang Y, Zhang L, Leonard M, Cui J, Gu H and Zhang X. Transcriptional Coactivator MED1 in Mammary Tumor Progression, Metastasis and Cancer Stem Cell Formation. Gordon Research Conferences: Stem Cells and Cancer, Apr. 21-26, 2013. Les Diablerets, Switzerland
  27. McKernan C, Zhang X and Greis K. Characterizing a Prominent Estrogen Receptor Coactivator, MED1, by Mass Spectrometry. UC and CCHMC Capstone Poster Session for Undergraduates. Aug 1-2, 2013, Cincinnati, OH
  28. Yang Y, Zhang L, Leonard M, Cui J, Gu H and Zhang X. Estrogen Receptor Coactivator MED1 in Breast Cancer Stem Cell Formation and Tumor Metastasis. ASCB Annual Meeting, Dec. 14-18, 2013. New Orleans, LA
  29. Charif M, Lower EE, Kennedy D, Kumar H, Khan S, Radhakrishnan N and Zhang X. The effect of trastuzumab therapy on clinical benefit from fulvestrant treatment for metastatic estrogen receptor positive breast cancer patients. ASCO Breast Cancer Symposium --- Enhancing Clinical Care Through Collaboration, Sept. 4-6, 2014. San Francisco, CA
  30. Leonard M, Zhang Y, Yang Y, Bi M, and Zhang X. Overcoming Tamoxifen Resistance of Human Breast Cancer using RNA Nanotechnology. UC Cancer Institute Breast Cancer Journey: Snapshot of Research, Nov. 1, 2014, Cincinnati, OH
  31. Bi M, Loring B, Cui J, Leonard M and Zhang X. Dual Regulation of MED1 and ErbB3 by miR-205-5p in the Tamoxifen Resistance of Human Breast Cancer. UC Cancer Institute Breast Cancer Journey: Snapshot of Research, Nov. 1, 2014, Cincinnati, OH
  32. Charif M, Lower EE, Kennedy D, Kumar H, Khan S, Radhakrishnan N and Zhang X. The effect of HER-2/neu overexpression on prolonging clinical benefit with fulvestrant treatment for metastatic estrogen receptor positive breast cancer patients treated with trastuzumab. San Antonio Breast Cancer Symposium, Dec. 9-12, 2014, San Antonio, TX

News Release:

  1. American Cancer Society: http://bit.ly/1Eq71hM
  2. Washington National: http://www.youtube.com/watch?v=DIoRmETPOHk
  3. UC HealthNEWs Featured: http://www.healthnews.uc.edu/news/?%2F24589
  4. WKRC TV12: http://www.local12.com/news/features/top-stories/stories/health-alert-indy-racecar-driver-tours-uc-biology-lab-11930.shtml
  5. UC HealthNEWs Featured: http://healthnews.uc.edu/news/?/23750/
  6. Fox 19 Live Interview: http://www.fox19.com/video?clipId=9546014&autostart=true
  7. PR Newswire:
    http://www.prnewswire.com/news-releases/hope-donates-100000-to-support-the-american-cancer-societys-national-research-program-230969911.html
  8. American Cancer Society: http://www.cancer.org/fight/100-stories-81-90 (Story 88)
  9. UC Dean’s List: https://www.healthnews.uc.edu/publications/deanslist/?/21402/
  10. UC HealthNEWs: http://healthnews.uc.edu/news/?/21288/
  11. UC HealthNEWs Featured: http://healthnews.uc.edu/news/?/14289/
  12. Susan G. Komen For the Cure: http://ww5.komen.org/KomenNewsArticle.aspx?id=19327353427
  13. UC Dean’s List: http://healthnews.uc.edu/publications/deanslist/?/14953/
  14. UC HealthNEWs: http://healthnews.uc.edu/news/?/21244/

 

Xiaoting Zhang

Associate Professor



  • :513-558-3017
  • DEPARTMENTDepartment of Cancer Biology University of Cincinnati College of Medicine
  • COUNTRY USA