Publications

Articles In Peer-Reviewed Journals

  1. Bandyopadhyay R, Bera T, Bhakat KK, Dutta C and Das J. Methyl directed DNA mismatch repair in Vibrio cholerae. J. Biosc. 19:557-564 (1994).
  2. Bhakat KK, Sharma MS and Das J. The mut K gene of Vibrio cholerae: a new gene involved in DNA mismatch repair. J. Bact. 181:879-883 (1999).
  3. Bhakat KK and Mitra S. Regulation of the human o6-methylguanine-DNA methyltransferase gene by transcriptional coactivators cAMP response element-binding protein-binding protein and p300. J. Biol. Chem. 275:34197-34204 (2000).
  4. Mitra S, Izumi T, Boldogh I, Bhakat KK, Hill JW and Hazra TK. Choreography of oxidative DNA damage repair in mammalian cells involving distinct protein ensembles. Invited Article in Free Radicals in Biology and Medicine 33:15-28 (2002).
  5. Bocangel DB, Finkelstein S, Schold SC, Bhakat KK, Mitra S and Kokkinakis DM. Multifaceted resistance of gliomas to temozolomide. Clin. Cancer Res. 8:2725-2734 (2002).
  6. Shimada Y, Nishimura M, Kakinuma S, Ogiu T, Fujimoto H, Kubo A, Nagai J, Kobayash K, Tano K, Yoshinaga S and Bhakat KK. Genetic susceptibility to thymic lymphomas and K-ras gene mutation in mice after exposure to X-rays and N-ethyl –N nitrosourea. Int. J. Radt. Biol. 79:423-430 (2003).
  7. Bhakat KK and Mitra S. CpG methylation-dependent repression of the human 06-methylguanine-DNA methyltransferase gene linked to chromatin structure alteration. Carcinogenesis, 24:1337-1345 (2003).
  8. Boldogh I, Roy G, Lee MS, Bacsi A, Hazra TK, Bhakat KK, Das G and Mitra S. Reduced DNA double strand breaks in chlorambucil resistant cells are related to high DNA-PKcs activity and low oxidative stress. Toxicology 193:137-152 (2003).
  9. Izumi T, Wiederhold LR, Roy G, Roy R, Jaiswal A, Bhakat KK, Mitra S and Hazra TK. Mammalian DNA base excision repair proteins: their interactions and role in repair of oxidative DNA damage. Toxicology 193:43-65 (2003).
  10. Bhakat KK, Izumi T, Yang SH, Hazra TK and Mitra S. Role of acetylated human AP-endonuclease (APE1/Ref-1) in regulation of the parathyroid hormone gene. EMBO J. 22: 6299-6309 (2003).
  11. Bhakat KK, Yang SH and Mitra S. Acetylation of human AP-endonuclease 1, a critical enzyme in DNA repair and transcription regulation. Methods in Enzymology, part D, 371:292-300 (2003).
  12. Bhakat KK, Hazra TK and Mitra S. Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activity. Nucleic Acids Research 32:3033-3039 (2004).
  13. Szczesny B, Bhakat KK, Mitra S and Boldogh I. Age-dependent modulation of DNA repair enzymes by covalent modification and subcellular distribution. Mech. Ageing Dev. 125:755-765 (2004).
  14. Izumi T, Brown DB, Naidu CV, Bhakat KK, MacInnes MA, Saito H, Chen| DJ and Mitra S. Two Essential but Distinct Functions of the Mammalian AP-Endonuclease. Proc. Natl. Acad. Sci. 102:5739-5743 (2005).
  15. Das A, Hazra TK, Boldogh I, Mitra S and Bhakat KK. Induction of the human oxidized base-specific DNA glycosylase NEIL1 by reactive oxygen species. J. Biol. Chem. 280:35272-35280 (2005).
  16. Bhakat KK, Mokkapati SK, Boldogh I, Hazra TK and Mitra S. Acetylation of human 8- oxoguanine-DNA glycosylase by p300 and its role in 8-oxoguanine repair in vivo. Mol. Cell. Biol. 26:1654-1665 (2006).
  17. Mitra S, Izumi T, Boldogh I, Chattopadhyay R, Bhakat KK and Szczesny B. Intracellular trafficking and regulation of mammalian AP-endonuclease 1 (APE1), an essential DNA repair protein. DNA Repair (Amst). 6:461-469 (2007).
  18. Das S, Chattopadhyay R, Bhakat KK, Boldogh I, Kohno K, Prasad R, Wilson SH and Hazra TK, Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress. J Biol Chem. 282:28474-28484 (2007).
  19. Fontijn D, Adema AD, Bhakat KK, Pinedo HM, Peters, GJ, Boven E. O6-Methylguanine-DNA-methyltransferase promoter demethylation is involved in basic fibroblast growth factor induced resistance against temozolomide in human melanoma cells. Mol Cancer Ther. 10:2807-2815 (2007).
  20. Evans PM, Zhang W, Chen X, Yang J, Bhakat KK and Liu C. Kruppel-likefactor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J Biol Chem. 282:33994-40002. (2007).
  21. Das S, Chattopadhyay R, Bhakat KK, Boldogh I, Kohno K, Prasad R, Wilson SH, Hazra TK. Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress. J Biol Chem. 282:28474-2884 (2007).
  22. Fontijn D, Adema AD, Bhakat KK, Pinedo HM, Peters GJ and Boven E. O6-methylguanine-DNA-methyltransferase promoter demethylation is involved in basic fibroblast growth factor induced resistance against temozolomide in human melanoma cells. Mol Cancer Ther. 10:2807-2815(2007).
  23. Fantini D, Vascotto C, Deganuto M, Bivi N, Gustincich S, Marcon G, Quadrifoglio F, Damante G, Bhakat KK, Mitra S and Tell G. APE1/Ref-1 regulates PTEN expression mediated by Egr-1. Free Radic Res. 42:20-29 (2008).
  24. Dou H, Theriot CA, Das A, Hegde ML, Matsumoto Y, Boldogh I, Hazra TK, Bhakat KK, and Mitra S. Interaction of the human DNA glycosylase NEIL1 with proliferating cell nuclear antigen. The potential for replication-associated repair of oxidized bases in mammalian genomes. J Biol Chem. 283:3130-3140 (2008).
  25. Zaky A, Busso C, Izumi T, Chattopadhyay R, Bassiouny A, Mitra S and *Bhakat KK. Regulation of the human AP-endonuclease (APE1/Ref-1) expression by the tumor suppressor p53 in response to DNA damage. Nucleic Acids Res. 36:1555-1566 (2008).
  26. Mantha AK, Oezguen N, Bhakat KK, Izumi T, Braun W and Mitra S. Unusual role of a cysteine residue in substrate binding and activity of human AP-endonuclease. J Mol Biol. 379:28-37(2008).
  27. Chattopadhyay R, Da, S, Maiti AK, Boldogh I, Xie J, Hazra TK, Kohno K, Mitra S and Bhakat KK. Regulatory role of human AP-endonuclease (APE1) in YB-1-mediated activation of the multidrug resistance Gene MDR1. Mol. Cell. Biol. 23:7066-7088 (2008).
  28. Bhakat KK, Mantha AK and Mitra S. Transcriptional regulatory functions of mammalian AP-endonuclease (APE1/Ref-1), an essential multifunctional protein. Antioxid Redox Signal. 11:621-637 (2009).
  29. Bhattaracharyya A, Chattopadhyay R, Burnette R, Cross J, Mitra S, Ernst P, Bhakat KK and Crowe S. Acetylation of Apurinic/Apyrimidinic Endonuclease-1 Regulates Helicobacter pylori-Mediated Gastric Epithelial Cell Apoptosis. Gastroentrology, 136:2258-2269 (2009).
  30. Konduri SD, Ticku J, Bobustuc GC, Sutphin RM, Colon J, Isley B, and Bhakat KK. Srivenugopal KS, Baker CH. Blockade of MGMT expression by O6 benzyl guanine leads to inhibition of pancreatic cancer growth and induction of apoptosis. Clin Cancer Res 15:6087-95 (2009).
  31. Bocangel D, Mitra S and Bhakat KK. p53-meidated downregulation of the human DNA repair gene O6-methylguanine-DNA-methyltransferase (MGMT) via interaction with Sp1 transcription factor. Anticancer Res. 29:3741-3750 (2009).
  32. Ray S, Lee C, Hou T, Bhakat KK, and Brasier AR. Regulation of signal transducer and activator of transcription 3 enhanceosome formation by apurinic/apyrimidinic endonuclease 1 in hepatic acute phase response. Mol. Endocrionol. 2:391-401 (2010).
  33. Sengupta S, Mantha AK Mitra S and Bhakat KK. Human AP-endonuclease (APE1/Ref1) and its acetylation regulate YB-1/p300 recruitment and RNA polymerase II loading in the drug induced activation of multidrug resistance gene MDR1. Oncogene, 30:482-493 (2011).
  34. Banerjee D, Mandal SM, Das A, Hegde ML, Das S, Bhakat KK, Boldogh I, Sarkar PS, Mitra S and Hazra TK. Preferential repair of oxidized base damage in the transcribed genes of mammalian cells. J Biol Chem. 286:6006-6016 (2011).
  35. Hegde ML, Mantha AK, Hazra TK, Bhakat KK, Mitra S and Szczesny B. Oxidative genome damage and its repair: Implications in aging and neurodegenerative diseases. Mech. Ageing Dev. 133:157-168 (2012).
  36. Sengupta S, Chattopadhyay R, Mantha AK Mitra S and Bhakat KK. Regulation of mouse renin gene by human AP-endonulcease (APE1/Ref-1) and HDAC1 corepressor complexes. Journal of Hypertension, 30:917-925 (2012).
  37. Lirussi L, Antoniali G, Vascotto C, D'Ambrosio C, Poletto M, Romanello M, Marasco D, Leone M, Quadrifoglio F, Bhakat KK, Scaloni A, and Tell G. Nucleolar accumulation of APE1 depends on charged lysine residues that undergo acetylation upon genotoxic stress and modulate its BER activity in cells. Mol Biol Cell, 23:4079-4096 (2012).
  38. Sengupta S, Mitra S and Bhakat KK. Dual Regulatory Roles of Human AP-Endonuclease (APE1/Ref-1) in CDKN1A/p21 Expression PLOS ONE 8 (7): e68467 (2013).

Book Chapters:

  1. Boldogh I, Bhakat KK, Bocangel D, Gokul C, Das CG and Mitra S. “Regulation of DNA Repair and Apoptosis by p53 and its impact on alkylating drug resistance of tumor cells.” In: DNA Repair in Cancer Therapy. Humana Press, 73-108 (2004).

Abstracts:

  1. Sengupta S, Mitra S and Bhakat KK. Dual Regulatory Roles of Human AP-endonuclease (APE-1/Ref-1) and its Relationship with p53 in CDKN1A/p21 Gene Expression. FASEB, SRC Snowmass Village, CO, July 15-20, 2012.
  2. Chattopadhyay R, Mitra S and Bhakat KK. Regulatory role of human AP-endonuclease (APE1) in YB-1-mediated activation of the multidrug resistance Gene MDR1. 3rd US/EU meeting on Endogenous DNA Damages and Repair, Moody Garden Hotel, Galveston, TX, Feb. 21-25, 2009.
  3. Bhakat KK, Chattopadhyay R and Mitra S. Lysine acetyaltion/deacetylation as a molecular switch for the mammalian AP-endonuclease (APE1) in transcription and DNA repair, FASEB summer research conferences, Histone deacetylase, Snowmass, CO, June 2-7, 2007.
  4. Bhakat KK, Chattopadhyay R, Izumi T, Mantha A and Mitra S. Acetylated APE1 is a repressor in calcium-mediated downregulation of the human renin gene. Abstract from scientific session, 2006, Circulation, 114:11-129, 2006.
  5. Bhakat KK, Mokkapati S, Boldogh I, Hazra TK and Mitra S. Posttranslational modification of 8-oxoguanine-DNA –glycosylase (OGG1). Mammalian DNA repair, Gordon Research conference, Ventura Beach Hotel, Ventura, Ca, Jan 16-21, 2005.
  6. Bhakat KK, Izumi T, Yang SH, Hazra TK and Mitra S. Role of acetylated human AP-endonuclease (APE1/Ref-1) in regulation of the parathyroid hormone gene. FASEB J. 18:C85-C85. Supplemental May 14, 2004.
  7. Bhakat KK and Mitra, S. Acetylation of DNA Glycosylases, involved in base excision repair. 1st US-EU DNA repair meeting on endogeneous stress, The National Conference Center, Lansdowne, VA, Oct-14-18, 2003.
  8. Bhakat KK, Izumi T, Yang SH, Hazra TK and Mitra S. In vivo acetylation of human AP-endonuclease 1 enhances its function as a trans-acting factor in Ca+2 –dependent repression of the parathyroid hormone gene. Maintenance of Genomic integrity, 55th annual symposium on fundamental Cancer research, M.D. Anderson Cancer Center, Houston, TX, Oct 15-18, 2002.
  9. Bhakat KK and Mitra S. Regulation of the human o6-methylguanine-DNA methyltransferase gene by transcriptional coactivators CBP/p300. Mammalian DNA repair, Gordon Research conference, Ventura Beach Hotel, Ventura, CA, Jan. 21-26, 2000.
  10. Bhakat KK and Mitra S. Regulation of the human o6-methylguanine-DNA methyltransferase gene by transcriptional coactivators CBP/p300. BER Workshop, Galveston, TX, 2000.
  11. Sengupta A, Bhakat KK and Das J. Methyl directed DNA mismatch repair in Vibrio cholerae. XVIth International Union of Biochemistry and Molecular Biology (IUBMB), held in New Delhi, India, Sept. 19-22, 1994, Vol. I, page 19.

Kishor Kumar Bhakat

Associate Professor

 

  • : 402-559-8467
    Fax: 402-559-7832
  • DEPARTMENTDepartment of Genetics, Cell Biology and Anatomy
    University of Nebraska Medical Center
  • COUNTRY USA