Abstract

This study was carried out to assess the feeding value of Moringa oleifera leaf meal (MOLM) in layers ration on feed intake, body weight (BW) change, feed conversion ratio, egg quality and shelf life. A total of 96 Koekoek hens aging 41 weeks were used and equally divided into 4 dietary treatments with a completely randomized design (CRD). Treatments are rations contained MOLM at the level of 0% (T₁), 5% (T₂), 10% (T₃) and 15% (T₄) with 3 replications. Body weight (BW) change was calculated by taking the difference of BW taken at start (initial BW) and end of the experiment (final BW). Data on feed intake and egg weight were recorded daily and mortality rate as it occurred throughout the experimental period. Egg quality was evaluated biweekly on 4 eggs per replicate. The shelf life of eggs was determined by considering albumen and yolk measurements as well as Haugh unit at an interval of 7 days on 4 eggs per replicate stored for 7, 14, 21 and 28 days. Albumen and yolk pH of eggs stored at 7, 14, 21 and 28 days was also determined to evaluate the shelf life. Parameters considered during the study showed a positive (P<0.05) response. Body weight change was 0.32 kg in T₁, 0.43 kg in T₂, 0.48 kg in T₃ and 0.37 kg in T₄. Feed conversion ratio (FCR (kg egg/kg feed)) was 1.73 in T₁, 2.10 in T₂, 1.52 in T₃ and 1.59 in T₄. BW change were recorded for T₃ while higher yolk color was observed for T₃ and average egg weight was 48.66 gm in T₁ , 54.51 gm in T₂, 49.94 gm in T₃ and 50.31 gm in T₄. Higher feed intake and T₄. In the present study, it was possible to prolong the age of eggs from hens that fed 5% MOLM than the control and other treatments. From most parameters considered in this study like feed intake, BW change and FCR; better egg quality and prolonged shelf life for internal egg quality, it is concluded that addition of 5% MOLM in the layers diet is recommended. Moringa oleifera leaf meal utilization at 5% in the poultry industry may serve the sector by enhancing the product quality as a feed additive besides serving as protein feed.

Keywords

Body weight change; Feed intake; Egg quality; Moringa oleifera

Introduction

Moringa (Moringa oleifera L.) is a widespread, multipurpose tree reported to have nutritional, therapeutic and prophylactic properties [1] and has several industrial applications which are derived from its leaves and fruits [2]. M. oleifera is commonly referred to as the Horseradish tree, Drumstick tree, Moringa, Mother’s best friend, West Indian bean and Bean oil [3]. It is the most widely cultivated species of a monogeneric family, Moringaceae, and includes 13 species of trees and shrubs native and distributed in sub-Himalayan regions of India, Srilanka, North Eastern and South Western Africa, Madagascar and Arabia [4]. In Ethiopia the tree is called as ‘Shiferaw’ or ‘Cabbage Tree’ and mostly found in the southern region.

M. oleifera is a rapidly growing tree which is easy to establish and cultivate. It is propagated either by planting or by seeding [5]. It also has good coppicing ability after pruning, and a capacity to produce high quantities of fresh biomass up to 120 tones dry matter/ha/year even at high planting densities [6]. M. oleifera is drought tolerant and tolerated annual precipitation of 500 to 1500 mm and annual temperatures from 18.7 to 28.5°C. M. oleifera is adapted to a wide range of soil types and conditions, neutral to slightly acidic soil (pH range of 5.0 to 6.5) it grows best in sandy soils, sensitive to water logging and frost.

Leaves of M. oleifera have a great value of nutrients thus indicated as the plant serve as a potential source of food supplement, used as a protein complement [7]. M. oleifera’s antioxidant properties alleviated the effect of stress in goats [2,8] evidenced by lower initial meat pH in goats supplemented with M. oleifera leaves compared to the ones fed on grass hay only [9]. The meat color can be modified by dietary antioxidants and minimize rancidity, retard lipid peroxidation, resulting in maintained meat quality and enhanced shelf life [10,11]. M. oleifera is endowed with some antioxidant properties [12,13]. Antioxidants are substances that may protect animal cells against the damage caused by free radicals that cause oxidation in the body [14]. Findings by researchers [15,16] indicated that M. oleifera leaves contain various phytochemicals: carotenoids, vitamins, chlorophyll, xanthins, minerals, amino acids, sterols, cytokines, alkaloids, flavonoids and phenolics. The presence of such phytochemicals might make M. oleifera a potential antioxidant. Administration of M. oleifera extract decreased hepatic marker enzymes and lipid peroxidation with simultaneous increase in the level of antioxidants as reported by Ashok and Pari, et al. [17]. Findings supported by Verma et al. [15] that the leaves of M. oleifera have phenols which are known to scavenge free radicals. M. oleifera leaves contain selenium and zinc [1] which is components of antioxidant enzymes. It is the need of this project to evaluate the importance of M. oleifera leaf meal (MOLM) on the quality and shelf life of chicken egg due to the nutritional composition reviewed.

Materials and Methods

Animals, Experimental Design and Treatments

The experiment was conducted at Debre Zeit Agricultural Research Center (DZARC), located 47 km far from southeast of Addis Ababa, Ethiopia. A total of 96 Koekoek 41 weeks old hens were in completely randomized design distributed into 12 pens with 8 hens in each pen and 3 replications.

The chemical compositions of feed ingredients were determined through proximate analysis (Table 1) from which experimental diets were prepared to be nearly isocaloric and isonitrogenous (Table 2), at ME of 2,750 kcal/kg of DM and 16.5% of CP. The hens were randomly assigned to four treatments: 0% MOLM (T₁, control), 5% MOLM (T₂), 10% MOLM (T₃), and 15% MOLM (T₄). Leaf was harvested from Moringa oleifera trees from an orchard found in DZARC poultry farm. The harvested leaves from the tree were twigged and spread out on a concrete floor and allowed to dry under shade and aerated conditions then run through a hammer mill with a size of 5 mm to produce the leaf meal.

Ingredients	Corn grain	Wheat middling	Soybean meal	Nougseed cake	MOLM6
1DM (%)	91.8	92.1	93.8	91.6	86.9
2CP (%DM)	8.32	16.3	40.8	33.8	29.2
3CF (%DM)	3.6	8.9	6.9	18.1	10.5
4EE (%DM)	4.3	5.0	6.3	7.3	5.6
Ash (%DM)	3.4	3.9	6.1	10.5	12.9
5ME (Kcal/kg DM)	3270	2038	2498	2230	2247
Calcium (%DM)	0.06	0.12	0.30	0.28	2.76
Phosphorus (%DM)	0.33	1.12	0.68	0.67	0.35
Beta carotene (mg/100g)	-	-	-	-	15.60

Table 1: Chemical composition of ingredients.
¹Dry Matter, ²Crude Proteins, ³Crude Fibers, ⁴Ether Extract, ⁵Metabolizable Energy, ⁶Moringa Oleifera Leaf Meal.

Ingredient (%)	Treatment
	T1	T2	T3	T4
Corn grain	60.00	60.00	60.00	59.00
Wheat middling	6.30	3.80	2.80	2.00
Nougseed cake	4.50	5.00	5.00	3.80
SBM	21.00	18.00	14.00	12.00
MOLM	0.00	5.00	10.00	15.00
Vitamin premix	0.50	0.50	0.50	0.50
Salt	0.30	0.30	0.30	0.30
Limestone	7.00	7.00	7.00	7.00
Methionine	0.10	0.10	0.10	0.10
Lysine	0.30	0.30	0.30	0.30
Total %	100	100	100	100
CP %	16.12	16.10	15.77	15.80
ME (kcal/kg DM)	2714	2711	2704	2691
DM %	90.68	89.76	89.60	89.48
Ash (% DM)	8.93	9.20	10.90	10.97
EE (% DM)	6.55	7.14	7.26	7.34
CF (% DM)	6.14	7.05	7.20	7.26

Table 2: Proportion (%) of ingredients used for formulating experimental diets.
MOLM: Moringa Oleifera Leaf Meal; SBM: Soybean Meal; T₁: No MOLM Inclusion; T₂: 5%; T₃: 10%; T₄: 15% MOLM of the Total Ration Substituting SBM; CP: Crude Protein; DM: Dry Matter, EE: Ether Extract, CF: Crude Fibre.

Experimental pens, laying nests, watering and feeding troughs were thoroughly cleaned, disinfected, and sprayed against external parasites before the onset of the experiment. Birds were fed twice a day (08:00 AM and 17:00 PM) and clean water was available at all times. Diets were offered 130 gm/ bird/day in a round feeder and water in a plastic fountain in a deep litter house covered with Tef straw. Fluorescent lamps of 20 lux were used for the lighting purpose for 4 hours; and 12 hours natural light. Before the commencement of the actual data collection, birds were adapted to respective treatment diet for a week.

Measurements

The experimental period lasted for 12 weeks during which the amount of feed offered to and refused from birds per pen was recorded daily and feed consumption calculated from the difference. At the start and end of the experiment hens were weighed and body weight (BW) change was calculated as the difference between the final and initial BW. Feed conversion ratio was determined as a unit egg weight per unit feed consumed. Mortality was recorded as it occurred.

Egg quality parameters were assessed at the middle and end of the experiment in terms of egg weight and shape index externally and the internal egg quality parameters were assessed by breaking eggs on a flat glass and separating each of the components such as shell weight, shell thickness, yolk color, yolk weight, yolk length, yolk height, yolk index, albumen weight, albumen height and Haugh unit. The shell, albumen and yolk were carefully separated and weighed individually. Shell weight and thickness were taken by removing the internal membrane. Shell thickness was measured as the average of the broad, middle and sharp points of the egg by using a digital micrometer. Albumen and yolk height were measured by tripod micrometer. Haugh unit (HU) was calculated using the formula (100 log10 (h+7.57−1.7w0.37, [18]: where; h=observed albumen height (mm), w=weight of egg (g). Yolk color was determined by Roche color fan measurement. Digital caliper was used to measure the length and width of the egg and the length of yolk, shape indexes were computed accordingly [19]

Egg shape index=width of egg/length of egg × 100

Yolk index=yolk height/yolk length

For the measurement of egg shelf life, eggs were stored for 1, 2, 3 and 4 weeks at room temperature, 12 eggs per treatment were randomly taken and albumen and yolk measurements were taken and their pH by using pH meter twice during the experimental period at the middle and end of the experiment [20].

Statistical analysis

Data were analyzed using the general linear model procedures of Statistical Analysis Systems software with the model containing treatments. One way ANOVA was employed. Differences between treatment means were separated using Tukey Kramer test [21].

Results

Feed consumption, body weight and feed conversion ratio

The effect of inclusion of MOLM at different levels on feed intake, BW change and FCR of dual purpose Koekeok hens is presented in table 3. Total feed intake was higher (P ≤ 0.05) for hens in T₁ and T₃ as compared to the others with the lowest intake recorded for T₂. However, the final BW was highest (P<0.05) for hens in T₂ than hens in T₄ and T₁ but statistically the same with that of T₃ . Similarly, hens fed 5% MOLM (T₂) had higher (P<0.05) BW change than hens in T₃ and T₄but not significantly differ (P>0.05) with T₁. The FCR (kg egg/kg feed) was higher (P<0.05) for T₂ than the rest of the treatments, with no differences (P>0.05) between the other treatments.

Parameters	Treatments (Means ± SEM)
	T1	T2	T3	T4	Sig.
TFI (kg)	10.73 ± 0.01a	10.16 ± 0.04c	10.73 ± 0.07a	10.37 ± 0.01b	*
IBW (kg)	1.54 ± 0.02a	1.54 ± 0.02a	1.54 ± 0.01a	1.54 ± 0.01a	NS
FBW (kg)	1.86 ± 0.01b	1.93 ± 0.02a	1.90 ± 0.01ab	1.77 ± 0.01c	*
BWC (kg)	0.32 ± 0.02ab	0.38 ± 0.01a	0.35 ± 0.01b	0.22 ± 0.02c	*
FCR (kg egg/kg feed)	1.73 ± 0.04b	2.10 ± 0.03a	1.52 ± 0.01b	1.59 ± 0.10b	*

Table 3: Feed intake, body weight change and feed conversion ratio of dual purpose Koekoek hens fed different levels of MOLM for 12 weeks.
*: P<0.05; Means followed by the same letter in rows do not differ statistically from one another by the Tukey test at 5% probability; MOLM: Moringa oleifera Leaf Meal; SBM: Soybean meal; T₁: Ration containing 0% MOLM; T₂: Ration containing 5% MOLM; T₃: Ration containing 10% MOLM; T₄: Ration containing 15% MOLM; NS: Non-significant; TFI: Total Feed Intake; IABW: Initial Average Body Weight; FABW: Final Average Body Weight; BWC: Body Weight Change; FCR: Feed Conversion Ratio.

Egg Quality

Results of egg quality parameters are presented in table 4. Egg length was higher for T₂ (P<0.05) than the others and was in the order of T₂ >T₃ >T₄ >T₁. Egg width was also higher (P<0.05) in T₂ than T₁, T₃ and T₄. Hens in T₃ and T₄ had higher (P>0.05) egg width than hens in T₁. Significantly higher (P<0.05) egg shape index was observed in T₁ than in T₂ and T₃ with no differences between T₂ and T₃, and T₃ and T₄, but hens in T₂ had less egg shape index than T₁ and T₄. Egg weight was higher (P<0.05) in T₂ than T₁, T₃and T₄; T₂ also had higher (P<0.05) egg shell weight and thickness than the others; T₁, T₃ and T₄ had no significant difference between each. Albumen height was higher (P<0.05) for T₂ than T₁, but was the same (P>0.05) with that of the rest of the treatments. Albumen weight was higher (P<0.05) for T₂ than all the remaining treatments. Consequently, Haugh unit was also higher (P<0.05) in T₂ than the rest of the treatments.

Parameters	Treatments
	T1	T2	T3	T4	Sig.
Egg shape index	80.92 ± 0.56a	75.80 ± 0.10c	77.23 ± 0.76bc	78.79 ± 0.25ab	*
Egg weight (g)	48.66 ± 0.36b	54.51 ± 0.47a	49.94 ± 0.91b	50.31 ± 0.33b	*
AST (mm)	0.29 ± 0.003b	0.38 ± 0.003a	0.31 ± 0.01b	0.30 ± 0.01b	*
Shell weight (g)	5.67 ± 0.33b	8.66 ± 0.33a	6.33 ± 0.66b	6.00 ± 0.57b	*
AH(mm)	5.33 ± 0.33b	7.33 ± 0.33a	5.66 ± 0.33ab	7.00 ± 0.57ab	*
AW (g)	21.67 ± 0.33c	29.66 ± 1.20a	25.33 ± 0.33b	23.33 ± 0.88cb	*
Haugh unit	75.33 ± 0.33b	87.33 ± 0.33a	79.00 ± 0.57b	77.67 ± 1.45b	*
Yolk weight (g)	15.33 ± 0.33b	20.66 ± 0.33a	17.00 ± 1.00b	17.33 ± 0.33b	*
Yolk color	1.00 ± 0.00c	8.66 ± 0.33b	11.33 ± 0.33a	11.33 ± 0.33a	*
Yolk index	0.27 ± 0.57b	0.34 ± 0.33a	0.33 ± 0.57ab	0.32 ± 0.33ab	*

Table 4: Effects of feeding different levels of MOLM as a substitute for SBM on egg quality parameters of dual purpose Koekoek hens.
*: P<0.05; Means followed by same letter in rows do not differ statistically from one another by the Tukey test at 5% probability. MOLM: M. oleifera leaf meal; SBM: Soybean meal; T₁: Ration containing 0% MOLM; T₂: Ration containing 5% MOLM; T₃: Ration containing 10% MOLM; T₄: Ration containing 15% MOLM; AST: Average Shell Thickness; AH: Albumen Height; AW: Albumen Weight; YL: Yolk Length; YH: Yolk Height.

Similarly, T₂ had higher (P<0.05) yolk weight than the rest of the treatments. Yolk color was higher (P<0.05) with the same value for T₃ and T₄ than that of T₁and T₂ . Hens in the control diet had the poorest yolk color than the rest of the treatments. Yolk length was highest (P<0.05) for T₂ than that of hens in the rest of the treatments. Hens in T₁ had the lowest yolk length as compared to that for T₃ and T₄, which had similar yolk length. Yolk height was the least for T₁ than the rest of the treatments, which had similar yolk height. Hens in T₂ had higher yolk index (P<0.05) than hens in T₁, but yolk index did not vary between T₂, T₃ and T₄; however, similar yolk index was observed between the control and T₃ and T₄.

Egg shelf life

Yolk and albumen pH: The effect of feeding different levels of dietary MOLM on yolk and albumen pH of eggs of dual purpose Koekoek hens is shown in table 5. The results revealed that the yolk pH of eggs stored for a week was the lowest (P<0.05) for T₂ than the rest of the treatments. Although T₁ and T₃ had similar yolk pH at 7 days of storage, but their yolk pH was lower than T₄. Also, the yolk pH of eggs stored for 2 weeks was the lowest (P<0.05) for T₂ than the rest of the treatments, but all other treatments had similar yolk pH. In the same way, the yolk pH of eggs stored for 3 weeks was lower (P<0.05) for T₂ than for T₄. But T₁ and T₃ were not significantly differing with T₄. The pH of eggs stored for 4 weeks was the lowest for T₂ than eggs in the rest of the treatments, but eggs in T₁ had higher (P<0.05) pH than eggs in T₃, which had no significant difference with pH of eggs in T₄.

Parameters	Treatments
	T1	T2	T3	T4	Sig.
Yolk pH, EST(d)
7	6.32 ± 0.05b	6.15 ± 0.01c	6.39 ± 0.03b	6.55 ± 0.02a	*
14	6.52 ± 0.09a	6.23 ± 0.01b	6.51 ± 0.01a	6.66 ± 0.008a	*
21	6.61 ± 0.13ab	6.29 ± 0.02b	6.61 ± 0.05ab	6.76 ± 0.01a	*
28	7.05 ± 0.10a	6.51 ± 0.01c	6.81 ± 0.02b	6.97 ± 0.02ab	*
Albumen pH, EST(d)
7	8.34 ± 0.08ab	8.18 ± 0.02b	8.32 ± 0.03ab	8.66 ± 0.18a	*
14	8.78 ± 0.06a	8.21 ± 0.02c	8.44 ± 0.01b	8.75 ± 0.003a	*
21	8.92 ± 0.01a	8.24 ± 0.008c	8.58 ± 0.02b	8.86 ± 0.03a	*
28	8.97 ± 0.05a	8.30 ± 0.04b	8.83 ± 0.01a	8.94 ± 0.008a	*

Table 5: Effects of MOLM on egg shelf life of dual purpose Koekeok hens in terms of yolk and albumen pH at 1, 2, 3 and 4 weeks of storage time.
*: P<0.05; Means followed by the same letter in rows do not differ statistically from one another by the Tukey test at 5% probability. MOLM: Moringa oleifera Leaf Meal; SBM: Soybean meal. T₁: Ration containing 0% MOLM; T₂: Ration containing 5% MOLM; T₃: Ration containing 10% MOLM; T₄: Ration containing 15% MOLM; EST: Egg Storage Time; d: Days.

At 7 days of storage, T₂ had lower mean albumen pH than T₄ but statistically not different (P>0.05) with T₁ and T₃. At 2 weeks of storage, T₁ and T₄ had higher (P<0.05) albumen pH than T₂ and T₃. Lower albumen pH was recorded for eggs from hens fed 5% MOLM (T₂) than the others. Similar trend was also observed on albumen pH that stored for 3 weeks of eggs obtained from hens that fed treatment diets. Higher (P<0.05) albumen pH was observed in T₁and T₄ than T₂ and T₃ with no significant difference (P>0.05) between T₁ and T₄. At 4 weeks of egg storage, lower mean value (P<0.05) was recorded in T₂ and there was no significant difference (P>0.05) observed among the others.

Albumen and yolk measurements: The effect of MOLM on yolk and albumen measurements at different storage times is summarized in table 6. The results indicated that different levels of dietary treatment of MOLM had significant effect on those parameters regarding with storage time. At 1 weeks of storage, higher (P<0.05) albumen height was recorded in T₂ than T₁ and T₃ but was not significantly different (P>0.05) with T₄. At 2 weeks of storage, T₂ showed a higher value than the others with no statistical difference (P>0.05) between T₃ and T₄, and T₃ and T₁. At 3 weeks of eggs storage from hens fed the treatment diets, higher value (P<0.05) of albumen height was recorded in T₂ than the rest. Besides, at 4 weeks of storage, lower value (P<0.05) of albumen height was observed in T₁ than the others but did not vary with T₄. It was observed that T₂ had a higher (P<0.05) albumen height than the rest of the treatments. Haugh unit calculated was affected by higher mean value of albumen height obtained in T₂. A higher and similar trend obtained in all the storage periods and it was significantly different (P<0.05) with the others. The trend in albumen weight was also similar as to the Haugh unit and albumen height in all the storage periods considered. It was revealed a higher (P<0.05) value in eggs obtained from birds fed 5% MOLM (T₂) than others. The albumen weight in T₁, T₃ and T₄ at a week of storage was not significantly (P>0.05) different.

Parameters	Treatments
	T1	T2	T3	T4	Sig.
Albumen height, EST(d)
7	6.00 ± 0.00b	8.00 ± 0.00a	6.00 ± 0.00b	7.00 ± 1.00a	*
14	4.66 ± 0.33c	8.00 ± 0.00a	5.33 ± 0.33cb	6.00 ± 0.00b	*
21	4.33 ± 0.33b	7.00 ± 0.00a	5.00 ± 0.00b	5.00 ± 0.00b	*
28	4.00 ± 0.00c	6.66 ± 0.33a	5.33 ± 0.33b	4.33 ± 0.33cb	*
Haugh unit, EST(d)
7	80.00 ± 0.00b	87.33 ± 0.33a	80.33 ± 0.33b	79.67 ± 0.33b	*
14	74.33 ± 0.33c	87.33 ± 0.33a	80.00 ± 0.57b	78.66 ± 0.33b	*
21	71.66 ± 0.33c	85.66 ± 0.33a	78.66 ± 0.33b	77.00 ± 0.57b	*
28	69.00 ± 0.57c	85.00 ± 0.57a	77.00 ± 0.57b	74.66 ± 0.66b	*
Albumen weight, EST(d)
7	22.00 ± 0.00b	30.00 ± 1.15a	23.00 ± 1.00b	23.67 ± 0.88b	*
14	18.66 ± 0.33c	30.00 ± 1.15a	21.00 ± 0.57cb	22.33 ± 0.33b	*
21	16.00 ± 0.57c	28.00 ± 0.57a	20.33 ± 0.33b	20.33 ± 0.33b	*
28	14.33 ± 0.33c	26.00 ± 0.57a	20.33 ± 0.33b	19.66 ± 0.33b	*

Table 6: Effects of MOLM on egg shelf life of dual purpose Koekoek hens in terms of albumen and Haugh measurements at different storage times (1, 2, 3 and 4 weeks).
*: P<0.05; Means followed by the same letter in rows do not differ statistically from one another by the Tukey test at 5% probability; MOLM: Moringa oleifera Leaf Meal; SBM: Soybean Meal; T₁: Ration containing 0% MOLM; T₂: Ration containing 5% MOLM; T₃: Ration containing 10% MOLM; T₄: Ration containing 15% MOLM; EST: Egg Storage Time; d: Days.

Effect of MOLM on egg shelf life in terms of yolk measurements at different storage times was presented in table 7. In all the periods (1, 2, 3 and 4 weeks of egg storage) the trend was similar and it observed a higher yolk weight in T₂ in the order of T₂ >T₃ >T₁ =T4 . At a week of storage, higher yolk length was observed in T₂ while lower in T₁ than T₃ and T₄. There was significant difference (P<0.05) among the treatments. At 2 weeks of storage, higher (P<0.05) mean value in yolk length was recorded in T₂ and lower in T₁ than the others. There was no difference (P>0.05) between T₃ and T₄. Likewise, the same happen at 3 weeks of storage. A higher value in yolk length was obtained in T₂ and lower in T₁ than the others at 4 weeks of storage also with a significant (P<0.05) difference among the treatments. Lower yolk height was obtained in T₁ than the others but it had no significant difference with T₄. Eggs in T₂ had higher mean yolk height (P<0.05) than T₁ but was not different between T₃ and T₃ at a week of storage. There was also no significant difference (P>0.05) between T₃ and T₄. At 2 weeks of storage, higher yolk height was obtained in T₂ and lower one was in T₁ which was lower (P<0.05) than T₃ and T₄ with non-significant difference between T₃ and T₄. Similarly, at 3 weeks of storage, T₂ had higher value than all the treatments and T₁ had lower mean value (P<0.05) than T₃ and T₃; there was no difference (P>0.05) between T₃ and T₄. At 4 weeks of storage, T₂ had higher yolk height (P<0.05) than the others and lower value was obtained in T₁ but was not variable (P>0.05) with T₄; there was no significant difference (P>0.05) between T₃ and T₄.

Parameters	Treatments				Sig.
	T1	T2	T3	T4
Yolk weight EST(d)
7	16.67 ± 0.33c	21.66 ± 0.33a	19.33 ± 0.33b	17.33 ± 0.33c	*
14	14.33 ± 0.33c	21.66 ± 0.33a	18.00 ± 0.57b	15.66 ± 0.33c	*
21	13.00 ± 0.57c	20.00 ± 0.57a	17.00 ± 0.57b	14.33 ± 0.33c	*
28	12.33 ± 0.33c	20.00 ± 0.57a	15.33 ± 0.33b	13.33 ± 0.33c	*
Yolk length EST(d)
7	37.38 ± 0.22d	47.50 ± 0.30a	42.63 ± 0.26b	41.19 ± 0.40c	*
14	36.39 ± 0.80c	47.50 ± 0.30a	41.02 ± 0.31b	40.15 ± 0.68b	*
21	33.13 ± 0.30c	45.42 ± 0.35a	40.14 ± 0.33b	38.61 ± 0.71b	*
28	31.62 ± 0.47d	45.42 ± 0.35a	38.99 ± 0.36b	36.75 ± 0.50c	*
Yolk height EST(d)
7	11.33 ± 0.33b	15.66 ± 0.33a	14.00 ± 0.57a	13.67 ± 0.88ab	*
14	10.33 ± 0.33c	15.66 ± 0.33a	13.33 ± 0.33b	13.33 ± 0.33b	*
21	9.33 ± 0.33c	15.00 ± 0.57a	12.33 ± 0.33b	11.33 ± 0.33b	*
28	9.00 ± 0.57c	15.00 ± 0.57a	11.33 ± 0.33b	10.33 ± 0.33cb	*

Table 7: Effects of MOLM on egg shelf life of dual purpose Koekoek hens in terms of yolk measurements at different storage times (1, 2, 3 and 4 weeks of storage).
*: P<0.05; Means followed by same letter in rows do not differ statistically from one another by the Tukey test at 5% probability; MOLM: Moringa oleifera Leaf Meal; SBM: Soybean meal; T₁: Ration containing 0% MOLM; T₂: Ration containing 5% MOLM; T₃: Ration containing 10% MOLM; T₄ : Ration containing 15% MOLM; EST: Egg Storage Time; d: days.

Discussion

Inclusion of MOLM in the diets of hens at different levels (5%, 10% and 15%) showed a significant effect on their feed intake, BW change and FCR in the current study. This was similar with the finding of Olugbemi et al. [22] who noted that addition of 10% and 20% MOLM to the laying hen diet increases these parameters. Similarly, Kakengi et al. [23] reported that substitution of MOLM for sunflower seed meal in ISA brown breed significantly increased feed intake and FCR. The result of the current study was also consistent with those reported for broilers, were supplementation with 5% MOLM showed significantly better FCR as compared to the 0%, 3% and 7% MOLM containing experimental diets [24]. But the present study did not agree with the finding of Etalem et al. [25] who noted that addition of MOLM on Dominant CZ layers at 5% had no effect on average feed intake, final BW and FCR. Olugbemi et al. [21] also found that addition of 5% MOLM to broilers’ diet had no significant effect on FCR and final BW when compared to a diet free of MOLM. The improvement in BW and FCR in the present study might be attributed to the rich content of nutrients in MOLM and antimicrobial properties of moringa.

Both internal and external egg quality in the present study were affected positively by the dietary substitution of SBM by MOLM at different levels in dual purpose Koekeok hens. Weight of sampled eggs and albumen weight, egg length, albumen height, yolk index, and yolk color, shell weight and yolk height, yolk weight were all improved especially at 5% MOLM inclusion. This was consistent with the finding of Etalem et al. [24] who reported a better egg quality obtained from hens fed 5% MOLM in the total ration. Improvement of albumen height in the present study was also agreed with the findings observed by Price, et al. [26] and Kaijage et al. [27] but inconsistent for improvement of yolk index. The higher the yolk index and Haugh unit, the more desirable is the egg quality [28] which resulted from the present study. This was also in agreement with the reports of Bhatnagar et al. [29] who conducted feeding MOLM to ISA brown breed at the levels of 5,10 and 20%, at lower levels improved egg quality and higher levels resulted in lower productivity and poorer egg quality indices. Improvement of egg weight in the current study was similar with Kakengi et al. [22] where substitution of sunflower with MOLM at 5% levels in the diet showed a positive effect on egg weight. The decrease in weight at higher levels of MOLM inclusion in the present study and others were also not clear but probably was due lower energy and CP availability and also associated with lower digestibility of CF component reported in various other leaf meals. Increasing of egg weight due to increase in weight of albumen and yolk especially for T₂ might be the main cause of improvement in Haugh Unit in the present study. This was also in agreement with Nobakht and Moghaddam, et al. [30] who noted a positive correlation between Haugh Unit and quality of egg components (yolk and albumen).

In the present study, the yolk color showed an increasing trend as the amount of MOLM increased in the ration. This was in line with the findings of Olugbemi et al. [21]; Etalem et al. [24] who observed that MOLM as a good pigmenting agent of poultry products due to its rich xanthophylls content. Egg yolk color is a very important factor in consumer satisfaction and influences human appetite [31], with a preference for golden yellow to orange yolk color [32]. Similarly, Jacob et al. [33] noted that yolk color is a key factor in any consumer survey relating to egg quality. The intense yellowish yolk color recorded in our study for eggs produced from birds on diets containing MOLM confirms its viability as a yolk-coloring agent, which can enhance the marketability of the eggs. Good shell thickness is an also important bio-economic trait in commercial egg production as it may help to reduce the percentage of cracked eggs and decrease the rate of loose for producers which supported by the present study.

The significant effect in shelf life of eggs in terms of pH of yolk and albumen at different storage time was in line with the findings of different researchers [34-37]. The decline in albumen and yolk pH deterioration rate was also characterized by Pappas et al. [38] as a function of the antioxidant status of egg contents. They proposed that organic selenium enhances the egg’s antioxidant status by upgrading the glutathione peroxidase activity in yolk and albumen. This in turns slows the process of lipid and protein oxidation during storage period; hence more valuable egg quality by extended storage time which is consistent with the antioxidant properties of M. oleifera [39,40] that observed in the present study. M. oleifera is among the most promising species based on their high antioxidant activity, high contents of micro-nutrients and phytochemicals that could help in stability and shelf life of poultry product [41]. The flavonoids such as quercetin and kaempferol were identified as the most potent antioxidants in moringa leaves [42]. Similarly, Siddhuraju and Becker, et al. [43] noted their antioxidant activity was higher than the conventional antioxidants such as ascorbic acid, which is also present in large amounts in moringa leaves and used to prolong shelf life of poultry products. The improvement in shelf life of eggs in the present study as a result of MOLM inclusion could be related to the presence of the antioxidants mentioned [44- 46] that phenolic compounds have a high antioxidant activity through three mechanisms: free-radical scavenging activity, transition-metal-chelating activity, and/or singlet-oxygen quenching capacity. Feeding of MOLM for dual purpose Koekoek hens in the present study improves the shelf life of an egg probably by minimizing the rapid increasing of the yolk pH. Similar probable reason also made [47] as some synergistic effects among ascorbic acid, α-tocopherol, and ß-carotene have been against oxidation. During storage of eggs, the pH of the albumen increases from oviposition 7.6 to 8.5 [48] and this is thought to be related to the deterioration of albumen quality. Li-Chan et al. [49] also noted during storage, the albumen pH increases at a temperature dependent rate to a maximum value of about 9.7. Different researchers [34-37] also noted a shift in albumen pH from 8.35 at day 7 to 9.08 and 9.29 after 14 and 21days of storage, respectively. This indicated that the result found in the present study was valuable regarding with improving shelf life of an egg in terms of pH of albumen. Yolk pH in the current study also lower than the value obtained by Li-Chan et al. [49] that reported in newly laid eggs, the yolk pH is in general close to 6.0; however, during storage it gradually increases to reach 6.4 to 6.9. This implies that, feeding of MOLM for dual purpose Koekoek hens in the present study improves the shelf life of an egg in terms of minimizing the rapid increasing of the yolk pH.

The decrease in albumen height, albumen weight and Haugh unit with storage time was consistent with the reports of Keener et al. [50] and Silversides and Budgell, et al. [38]. Storage time has inverse relationship with height of albumen. During egg storage, the quality of the vitelline membrane declines, making the yolk more susceptible to breaking and decreases its weight [51]. In the current study, there was a decrease in yolk weight but there was no breakage except eggs from the control group. This implies that moringa leaf may have a promising potential in extending the shelf life of poultry products. Niekerk, et al. [52] reported that storage for one week at 25℃ will reduce the Haugh unit up to the limit of acceptable freshness (70 HU), whereas storage for one week at 8℃ will result in eggs that are still very fresh (85 to 90 HU). This indicated that eggs from hens fed MOLM in the present study were still under the grade of AA according to USDA [53] up to four week storage at room temperature.

The significant decrease of yolk and albumen measurements relating with storage time was in line with the finding of Jin et al. [54]; Gavril and Usturoi, et al. [55] and Tebesi et al. [56]. The range of values observed in the current study differed from values reported by Tayeb, et al. [57] on effects of storage length on egg quality parameters of laying hens. Wojdylo et al. [58] noted that many herbs, spices, and their extracts have high antioxidant capacity, such as, moringa (M. oleifera), Oregano (O. vulgare), Rosemary (R. officinalis), and Sage (S. officinalis) to improve quality of poultry products in terms of prolonging their shelf life and this was in line with the current study.

Conclusion and Recommendations

• Inclusion of MOLM at 5% (T₂) dietary level improved BW change, feed conversion ratio, egg quality parameters (egg length, egg width, egg shape index, albumen weight and height, yolk weight and height, Haugh unit, yolk color, yolk length and yolk index) and the egg shelf life.
• The study showed that obtained responses due to inclusion of MOLM at 5% suggest the shrub to have a potential as alternative protein feed ingredient and as well as it may serve as a feed additive to keep the product quality in the poultry sector.

Acknowledgements

The authors gratefully acknowledge financial support from the Ethiopian Institute of Agricultural Research (EIAR) and DZARC for hosting and offering the whole experimental facilities.

References

1. Moyo B, Masika PJ, Hugo A, Muchenje V (2011) Nutritional characterization of moringa (Moringa oleifera Lam.) leaves. Afr J Biotechnol 10: 12925-12933. [Ref.]
2. Anwar F, Latif S, Ashraf M, Gilani AH (2007) Moringa oleifera: a food plant with multiple medicinal uses. Phytother Res 21: 17-25. [Ref.]
3. Fahey J (2005) Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1. Trees Life Journal 1: 5-15. [Ref.]
4. Monera TG, Wolfe AR, Maponga CC, Benet LZ, Guglielmo J (2008) Moringa oleifera leaf extracts inhibit 6beta-hydroxylation of testosterone by CYP3A4. J Infect Dev Ctries 2: 379-383. [Ref.]
5. Aregheore EM (2002) Intake and digestibility of Moringa oleifera–batiki grass mixtures by growing goats. Small Rumin Res 46: 23-28. [Ref.]
6. Abou Elezz Fouad Mohammed K, Sarmiento Franco L, Santos Ricalde R, Solorio Sanchez JF (2011) Nutritional effects of dietary inclusion of Leucaena leucocephala and Moringa oleifera leaf meal on Rhode Island Red hens’ performance. Cuban J Agric Sci 45: 163-169. [Ref.]
7. Mendieta Araica B, Spörndly R, Reyes-Sànchez N, Spörndly E (2011) Moringa (Moringa oleifera) leaf meal as a source of protein in locally produced concentrates for dairy cows fed low protein diets in tropical areas. Livestock Sci 137: 10-17. [Ref.]
8. Robbins K, Jensen J, Ryan KJ, Homco-Ryan C, McKeith FK, et al. (2003) Dietary vitamin E supplementation effects on the color and sensory characteristics of enhanced beef steaks. Meat Sci 64: 279-285. [Ref.]
9. Moyo B, Masika PJ, Muchenje V (2014) Effect of feeding moringa (Moringa oleifera) leaf meal on the physic-chemical characteristics and sensory properties of goat meat. S. Afr. j. anim. Sci 44: 64-70. [Ref.]
10. Jang A, Liu XD, Shin MH, Lee BD, Lee SK, et al. (2008) Antioxidative potential of raw breast meat from broiler chicks fed a dietary medicinal herb extract mix. Poult Sci 87: 2382-2389. [Ref.]
11. Nkukwana TT, Muchenje V, Masika PJ, Hoffman LC, Dzama K, et al. (2014) Fatty acid composition and oxidative stability of breast meat from broiler chickens supplemented with Moringa oleifera leaf meal over a period of refrigeration. Food Chem 142: 255-261. [Ref.]
12. Atawodi SE, Atawodi JC, Idakwo GA, Pfundstein B, Haubner R, et al. (2010) Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J Med Food 13: 710-716. [Ref.]
13. Moyo B, Oyedemi S, Masika PJ, Muchenje V (2012) Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Meat Sci 91: 441-447. [Ref.]
14. Tsang C, Higgins S, Duthie GG, Duthie SJ, Howie M, et al. (2005) The influence of moderate red wine consumption on antioxidant status and indices of oxidative stress associated with CHD in healthy volunteers. Br J Nutr 93: 233-240. [Ref.]
15. Verma AR, Vijayakumar M, Mathela CS, Rao CV (2009) In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food Chem Toxicol 47: 2196-2201. [Ref.]
16. Falowo AB, Fayemi PO, Muchenje V (2014) Natural antioxidants against lipid–protein oxidative deterioration in meat and meat products: A review. Food Res Int 64: 171-181. [Ref.]
17. Ashok Kumar N, Pari L (2003) Antioxidant action of Moringa oleifera Lam. (drumstick) against antitubercular drugs induced lipid peroxidation in rats. J Med Food 6: 255-259. [Ref.]
18. Keener KM, LaCrosse JD, Curtis PA, Anderson KE, Farkas BE (2000) The influence of rapid air cooling and carbon dioxided cooling and subsequent storage in air and carbon dioxide on shell egg quality. Poult Sci 79: 1067-1071. [Ref.]
19. Ebrahimi MR, Jafari Ahangari Y, Zamiri MJ, Akhlaghi A, Atashi H (2012) Does preincubational in ovo injection of buffers or antioxidants improve the quality and hatchability in long-term stored eggs? Poult Sci 91: 2970-2976. [Ref.]
20. Olugbenga A, Yinka P, Emem A, Mutiu O (2015) Lipid Profile of Eggs from Laying Chickens Fed Five Proprietary Vitamin-Mineral Premixes under Two Rearing Systems as Influenced by Duration of Storage. Food and Public Health 5: 10-16. [Ref.]
21. Statistical Analysis System (SAS) (2009) Statistical Analysis Systems for mixed models. SAS Institute Inc, Cary, North Carolina, USA.
22. Olugbemi T, Mutayoba S, Lekule F (2010) Effect of Moringa (Moringa oleifera) Inclusion in Cassava Based Diets Fed to Broiler Chickens. Int J Poult Sci 9: 363-367. [Ref.]
23. Kakengi A, Kaijage J, Sarwatt S, Mutayoba S, Shem M, et al. (2007) Effect of Moringa oleifera leaf meal as a substitute for sunflower seed meal on performance of laying hens in Tanzania. Livestock Research for Rural Development 9: 363-367. [Ref.]
24. Safa M, Tazi E (2014) Effect of feeding different levels of Moringa oleifera leaf meal on the performance and carcass quality of broiler chicks. Int J Sci Res 3: 147-151. [Ref.]
25. Tesfaye E, Animut G, Urge M, Dessie T (2014) Cassava root chips and Moringa oleifera leaf meal as alternative feed ingredients in the layer ration. J Appl Poult Res 23: 614-624. [Ref.]
26. Price LM (2000) The moringa tree. [Ref.]
27. Kaijage J, Sarwatt S, Mutayoba S (2004) Moringa oleifera leaf meal can improve quality characteristics and consumer preference of marketable eggs. 126-129.
28. Fayeye T, Adeshiyan A, Olugbami A (2005) Egg traits, hatchability and early growth performance of Fulani-ectotype chicken. Livestock Research for Rural Development 17. [Ref.]
29. Bhatnagar R, Kataria M, Verna S (1996) Effects of dietary leuceana leaf meal on the performance and egg characteristics in White Leghorn hens. Indian J Anim Sci 66: 1291-1294.
30. Nobakht A, M Moghaddam (2012) The effects of different levels of costmary (Tanacetum balsamita) medicinal plant on performance, egg traits and blood biochemical parameters of laying hens. Iranian J Anim Sci 27: 125-130. [Ref.]
31. Amerine MA, Pangborn RM, Roessler EB (1995) Principles of sensory evaluation of food. New York, 56. [Ref.]
32. Hasin BM, Ferdaus AJM, Islam MA, Uddin MJ, Islam MS (2006) Marigold and Orange Skin as Egg Yolk Color Promoting Agents. Int J Poult Sci 5: 979-987. [Ref.]
33. Jacob JP, Miles RD, Mather FB (2000) Egg Quality. University of Florida, 1-12. [Ref.]
34. Scott TA, Silversides FG (2000) The effect of storage and strain of hen on egg quality. Poult Sci 79: 1725-1729. [Ref.]
35. Silversides FG, Scott TA (2001) Effect of storage and layer age on quality of eggs from two lines of hens. Poult Sci 80: 1240-1245. [Ref.]
36. Monia KN, Salahuddin M, Miah G (2003) Effect of breed and holding period on egg quality characteristics of chicken. Int J Poult Sci 2:261-263. [Ref.]
37. Silversides FG, Budgell K (2004) The relationships among measures of egg albumen height, pH, and whipping volume. Poult Sci 83: 1619-1623. [Ref.]
38. Pappas AC, Karadas F, Surai PF, Speake BK (2005) The selenium intake of the female chicken influences the selenium status of her progeny. Comp Biochem Physiol B Biochem Mol Biol 142: 465-474. [Ref.]
39. Thomson M, Ali M (2003 Garlic [Allium sativum]: a review of its potential use as an anti-cancer agent. Curr Cancer Drug Targets3: 67-81. [Ref.]
40. Mirunalini S, Kumaraguruparan R, Subapriya R, Nagini S (2004) Garlic oil enhances hepatic and blood antioxidants during hamster buccal pouch carcinogenesis. J Pharm Biol 42: 240-245. [Ref.]
41. Jung S, Choe JH, Kim B, Yun H, Kruk ZA, et al. (2010) Effect of dietary mixture of gallic acid and linoleic acid on antioxidative potential and quality of breast meat from broilers. Meat Sci 86: 520-526. [Ref.]
42. Foidl N, Makkar H, Becker K (2001) The potential of Moringa oleifera for agricultural and industrial uses. Agritrop 45-76. [Ref.]
43. Siddhuraju P, Becker K (2003) Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agric Food Chem 15: 2144-2155. [Ref.]
44. Zheng G, Xu L, Wu P, Xie H, Jiang Y, et al.(2009) Polyphenols from longan seeds and their radical-scavenging activity. Food Chem 116: 433-436. [Ref.]
45. Andjelkovic M, Van Camp J, Meulenaer BD, Depaemelaere G, Socaciu C, et al. (2006) Iron-chelation properties of phenolic acids bearing catechol and galloyl groups. Food Chem 98: 23-31. [Ref.]
46. Mukai K, Nagai S, Ohara K (2005) Kinetic study of the quenching reaction of singlet oxygen by tea catechins in ethanol solution. Free Radic Biol Med 39: 752-761. [Ref.]
47. Yeum KJ, Beretta G, Krinsky NI, Russell RM, Aldini G (2009) Synergistic interactions of antioxidant nutrients in a biological model system. Nutrition 25: 839-846. [Ref.]
48. Heath JL (1977) Chemical and related osmotic changes in egg albumen during storage. Poult Sci 56: 822-828. [Ref.]
49. Li-chan E, Powrie W, Nakai S (1995) The chemistry of egg and egg products. 105-175.
50. Keener KM, LaCrosse JD, Curtis PA, Anderson KE, Farkas BE. (2000) The influence of rapid air cooling and carbon dioxided cooling and subsequent storage in air and carbon dioxide on shell egg quality. Poult Sci 79: 1067-1070. [Ref.]
51. Kirunda DF, McKee SR (2000) Relating quality characteristics of aged eggs and fresh eggs to vitelline membrane strength as determined by a texture analyzer. Poult Sci 79: 1189-1193. [Ref.]
52. Niekerk TV (2014) Egg quality. Low Input Breeds, technical note, 24. [Ref.]
53. United State Departments of Agriculture (USDA) (2000) United States Standards, Grades and Weight Classes for Shell Eggs. USDA, Washington, DC. [Ref.]
54. Jin YH, Lee KT, Lee WI, Han YK (2011) Effects of storage temperature and time on the quality of eggs from laying hens at peak production. Asian-Australas J Anim Sci 24: 279-284. [Ref.]
55. Gavril R, Usturoi MG (2012) Effect of storage time and temperature on hen egg quality. Seria Zootehnie 57: 221-229. [Ref.]
56. Tebesi T, Madibela OR, Moreki JC (2012) Effect of Storage Time on Internal and External Characteristics of Guinea Fowl (Numida meleagris) Eggs. J Anim Sci Adv 2: 534-542. [Ref.]
57. Tayeb T (2012) Effects of storage temperature and length on egg quality parameters of laying hen. J Anim Sci 1: 32-36.
58. Wojdylo A, Oszmianski J, Czemerys R (2007) Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 105: 940-949. [Ref.]

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Article Information

Article Type: RESEARCH ARTICLE

Citation: Tesfaye E, Alebachew W, Tamir B (2018) Performance of Koekoek Chicken Fed with Different Levels of Moringa oleifera Leaf Meal. J Anim Sci Res 2(3): dx.doi.org/10.16966/2576-6457.115

Copyright: © 2018 Tesfaye E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication history: 

Received date: 17 Apr, 2018

Accepted date: 11 May, 2018

Published date: 18 May, 2018